Transmission of peanut yellow spot virus (PYSV) by Thrips, Scirtothrips dorsalis Hood in groundnut

Peanut yellow spot virus (PYSV) was efficiently transmitted by Scirtothrips dorsalis Hood in groundnut. Larvae could acquire the virus in 30 min and the maximum percentage transmission of 43.8% by individual insects resulted following two days AAP. Single adult Thrip transmitted the virus after minimum IAP of 30 minutes. The percentage transmission (33.3%) increased linearly with an increase in IAP up to 1.5 days and maximum up to 55 h of IAP (36.1%). PYSV persistently transmitted more than 75% of their life spa

Kharif Sorghum Germplasm Collection in Karnataka and Adjoining Areas

An assessment of the present world collection of sorghum maintained at ICRISAT reveals that the majority of the germplam constitute either experimental accessions which are generally photo insensitive or authentic indigenous races from tropical countries which are photosensitive. Most of the landraces assembled from tropical countries including India behave photoperiod sentitive when grown in a long day (rainy) seson at Patancheru (17 27'N latitude) indicating the lacking of early insensitive types especially from India. There are very few pockets in South India where early kharif types are cultivated among which Northern Karnataka and its adjoining areas are very important. These areas were not adequately collected in earlier missions and their valuble germplasm are facing severe threat from hybrid and improved sorghum varieties. More landraces from this region may provide valuable source material for sorghum improvement programs particularly for earliness probable resistance to drought (several areas are drought prone). They may also possess weathering resistance because of their maturity during rainy seaso

Kloniranje, ekspresija i karakterizacija paraflagelarnog gena Rod 2 bičaša Trypanosoma evansi

Paraflagellar rod is the major structural component of the trypanosomatid flagellum and is identified as a complex lattice of filaments which runs parallel to the axoneme throughout most of the flagellar length. The present study was carried out to investigate the existence of the paraflagellar rod (PFR 2) gene in Trypanosoma evansi infecting Indian cattle. Local isolates of T. evansi collected from naturally infected cow were multiplied in Wistar rats. Complementary DNA (cDNA) was synthesized from the RNA of host cell free T. evansi parasites by reverse transcription. The gel purified PCR product (PFR 2 gene of T. evansi) was cloned into the pTZ57R/T vector system. The nucleotide sequence of the PFR 2 gene of the T. evansi S.V.V.U. isolate (Accession No. KT277497) obtained in the present study revealed 100% homology with T. evansi China isolate and 99% homology with T. evansi Izatnagar and Bikaner isolates. The recombinant protein was sub-cloned into pET 32a and expressed in the BL21 (DE3) pLysS expression system. The PFR 2 gene of T. evansi S.V.V.U. isolate was further characterized by determination of its protein profile with SDS-PAGE and western blotting. Indirect ELISA was optimized for detection of the specific antibody titre against the recombinant protein of the PFR 2 gene of T. evansi. In the kinetoplastid species the PFR 2 gene is highly conserved. Therefore the PFR 2 gene was suggested as a vaccine candidate, as well as a diagnostic antigen.Paraflagelarni štapić glavna je strukturna komponenta tripanosomskog biča i dio je kompleksa filamenaza koji teku paralelno s aksonemom duž biča. Istraživanje je provedeno kako bi se ispitalo postojanje paraflagelarnog gena Rod 2 (PFR2) u bičaša Trypanosoma evansi koji invadira goveda u Indiji. Lokalni izolat T. evansi prikupljen od prirodno invadiranih krava umnožen je u Wistar štakora. Komplementarna DNA (cDNA) sintetizirana je iz RNA obrnutom transkripcijom iz stanica neinvadiranih nositelja T. evansi parazita. Pročišćeni PCR produkt (gen PFR2 bičaša T. evansi) kloniran je u vektorski sustav pTZ57R/T. Nukleotidna sekvencija gena PFR2 bičaša T. evansi, izolat S.V.V.U. (pristupni broj KT277497) dobivena u ovom istraživanju pokazala je 100 %-tnu sličnost s izolatom T. evansi China i 99 %-tnu s izolatom T. evansi Izatnagar i Bikaner. Rekombinantni protein ponovno je kloniran u sustavu pET 32a i prikazan u sustavu BL21 (DE3) pLysS. Gen PFR2 bičaša T. evansi, izolat S.V.V.U. dalje je karakteriziran određivanjem proteinskog profila metodama SDS-PAGE i Western blotting. Indirektni test ELISA optimiziran je za dokaz titra specifičnih protutijela za rekombinantni protein gena PFR2 bičaša T. evansi. U kinetoplastida gen PFR2 izrazito je očuvan. Stoga bi se gen PFR2 mogao upotrijebiti za cjepivo te kao dijagnostički antigen

Massive spontaneous corpus callosal hemorrhage with intraventricular extension

Spontaneous hemorrhage into the corpus callosum with intraventricular extension is uncommon. In the present article we describe a case 60 year female who had massive hemorrhage along the corpus callosum involving genu, body of corpus callosum and extending on ventral as well as dorsal aspect of the corpus callosum with intraventricular extension and areas of hypodensities in the body of the corpus callosum and adjacent cerebral cortex. In this case the subarachnoid and corpus callosal hemorrhage probably resulted from the ruptured aneurysms of the anterior cerebral artery

Morphological diversity in sorghum germplasm from India

Of the 5000 sorghum (Sorghum bicolor L. Monench) germplasm accessions assembled at ICRISAT Asia Center (IAC), over 4000 accessions from 11 major sorghum growing states in India were evaluated for morphological and agronomical characters at IAC during rainy and postrainy seasons. Considerable diversity was observed for all the characters studied. Diversity was more among states than within states. Variation among the seasons was also considerable. Days to flowering ranged from 42 to 129 days during postrainy and 33 to 180 days during the rainy season indicating their day length sensitivity. Plant height ranged from 65 to 330 cm during postrainy and 75 to 655 cm during rainy season. Panicle length ranged from 5 cm to 52 cm and panicle width from 1 to 51 cm. Erect and compact panicles are more frequent although all possible panicle shapes and compactness were found. In general, sorghums from India produce medium to large, lustrous grains in which the subcoat (testa) is absent. All the 5 basic and 10 intermediate sorghum races were found in India though durra or half-durra predominate. Racial diversity is maximal in the state of Andhra Pradesh followed by Maharashatra state. In the world collection a majority of the sources of resistance to shoot fly, stem borer, and striga are from India. Based on diversity for morphological and agronomical characters, India could be considered as the secondary center of diversity. Sorghum germplasm from India is useful for good quality large grai

Developing proso millet (Panicum miliaceum L.) core collection using geographic and morpho-agronomic data

Proso millet (Panicum miliaceum L.) is a rich source of protein, minerals, and vitamins, and is an important cereal crop of Asia and Africa. Due to its lowest water and nutrient requirement, it has the potential for agriculture diversification. The development of a core collection would assist in efficient management and enhanced utilisation of proso millet genetic resources. The present investigation was conducted to develop a core collection of proso millet based on geographic information and 20 qualitative and quantitative traits recorded on 833 accessions conserved in the International Crops Research Institute for the Semi-Arid Tropics genebank. The entire germplasm collection was stratified into five groups based on races and data on 20 morpho-agronomic traits were used for clustering following Ward’s method. About 10% (or at least one accession) was randomly selected from each of 101 clusters to constitute a core collection of 106 accessions. Comparisons of means, variances, frequency distribution, diversity indices, and correlation studies indicated that the variation in the entire collection has been preserved in the core collection. This core collection provides a gateway to identify diverse trait-specific germplasm accessions for important agronomic traits and for abiotic and biotic stresses for use in crop improvement research and in crop diversification programs

Characteristics of spectral aerosol optical depths over India during ICARB

Spectral aerosol optical depth (AOD) measurements, carried out regularly from a network of observatories spread over the Indian mainland and adjoining islands in the Bay of Bengal and Arabian Sea, are used to examine the spatio-temporal and spectral variations during the period of ICARB (March to May 2006). The AODs and the derived Angstrom parameters showed considerable variations across India during the above period. While at the southern peninsular stations the AODs decreased towards May after a peak in April, in the north Indian regions they increased continuously from March to May. The Angstrom coefficients suggested enhanced coarse mode loading in the north Indian regions, compared to southern India. Nevertheless, as months progressed from March to May, the dominance of coarse mode aerosols increased in the columnar aerosol size spectrum over the entire Indian mainland, maintaining the regional distinctiveness. Compared to the above, the island stations showed considerably low AODs, so too the northeastern station Dibrugarh, indicating the prevalence of cleaner environment. Long-range transport of aerosols from the adjoining regions leads to remarkable changes in the magnitude of the AODs and their wavelength dependencies during March to May. HYSPLIT back-trajectory analysis shows that enhanced long-range transport of aerosols, particularly from the west Asia and northwest coastal India, contributed significantly to the enhancement of AOD and in the flattening of the spectra over entire regions; if it is the peninsular regions and the island Minicoy are more impacted in April, the north Indian regions including the Indo Gangetic Plain get affected the most during May, with the AODs soaring as high as 1.0 at 500 nm. Over the islands, the Angstrom exponent (α) remained significantly lower (~1) over the Arabian Sea compared to Bay of Bengal (BoB) (~1.4) as revealed by the data respectively from Minicoy and Port Blair. Occurrences of higher values of α, showing dominance of accumulation mode aerosols, over BoB are associated well with the advection, above the boundary layer, of fine particles from the east Asian region during March and April. The change in the airmass to marine in May results in a rapid decrease in α over the BoB

Prospects of new chickpea varieties in Andhra Pradesh

Andhra Pradesh is an important chickpea growing state in southern India, with spectacular increase in chickpea area from 120,000 ha in 1997/98 to 638,000 ha in 2007/08. The chickpea revolution in Andhra Pradesh has improved the prospects of many resource-poor, small land holding and rainfed farmers of Andhra Pradesh. However, the growing season of chickpea in Andhra Pradesh is warm and short (90-110 days), and drought is the foremost factor responsible for significant yield losses. Rainfall in major chickpea-growing regions is quite uncertain and erratic, resulting in poor yields. The Regional Agricultural Research Station of Acharya N G Ranga Agricultural University, Nandyal, Andhra Pradesh, India is the lead centre responsible for location-specific research in chickpea in Andhra Pradesh. With support from ICRISAT and ICAR, the centre has initiated crop improvement programmes during 2004 and has released four promising chickpea varieties for commercial cultivation. Three desi varieties viz., Nandyal Sanaga 1(NBeG 3), Dheera (NBeG 47), and Nandyal Gram 49 (NBeG 49) released for Andhra Pradesh and one large-seeded kabuli Nandyal Gram 119 (NBeG 119) released for the southern zone comprising Andhra Pradesh, Karnataka and Tamil Nadu, are cutting across chickpea growing regions of Andhra Pradesh. Nandyal Sanaga 1, released in 2012, is a bold-seeded desi variety tolerant to drought and heat; Dheera released during 2015 is also a desi variety and the first of its kind in India, suitable for mechanical harvesting. Nandyal Gram 49 released during 2016 is a highyielding desi variety with attractive seeds; whereas Nandyal Gram 119 is early bold-seeded kabuli variety released during 2015. These varieties have clearly demonstrated their advantage (10%-15 % increase over popular varieties of the tract) in farmers’ holdings in large-scale demonstrations and are being preferred by farmers of not only Andhra Pradesh, but also Karnataka, Tamil Nadu, Odisha and Maharastra. Efforts are underway to promote large-scale adoption of these varieties to maximize long term productivity of chickpeas in rainfed vertisols

RP-HPLC Method for the Simultaneous Analysis of Ambroxol Hydrochloride and Nitazoxanide in API and Tablet Dosage Form

Present work is aimed to develop a new simple, fat, rapid, accurate, efficient, and reproducible RP-HPLC method for the simultaneous analysis of Ambroxol Hydrochloride and Nitazoxanide in API& tablet dosage form. The chromatographic separation was performed using phenomenex C18 Column having dimensions of 4.6x250mm having particle size of 5 μm, with mobile phase consisting of Buffer PH-3.5 and Acetonitrile (40:60% v/v), flow rate was adjusted to 1.0ml/min and detection wavelength at 235 nm. The proposed method has been validated for linearity, range, precision, accuracy and robustness were within the acceptance limit according to the ICH Q2B guidelines. The retention times of Ambroxol Hydrochloride and Nitazoxanide were 2.985 mins and 5.581 mins respectively. The linearity was performed in the concentration in the range of 7.5 μg/ml to 45 μg/ml and 25 μg/ml to 150 μg/ml and with a correlation coefficient of 0.999 and 0.999 respectively. % RSD for system precision was found to be 0.212 and 0.160% RSD for repeatability 0.2 and 0.12, % RSD for intermediate precision was 0.06 and 0.06 respectively. The % percentage purity of Ambroxol Hydrochloride and Nitazoxanide was found to be 99.93% and 99.35% respectively. The method was found to be robust even by change in the mobile phase ±5% in less flow condition. © 2022 Author(s)