Effect of differential heat treatments on antibacterial activity of fermented goat milk

The present study was conducted to evaluate the effect of three different temperatures on the antibacterial potential of fermented goat milk.Goat milk is one of the naturalfoods to complete perfection.Bioactive peptides, the specific protein fragments that have a positive impact on body functions, can be produced during milk fermentation. Thanks to the starter cultures’ proteolytic activity.Fresh pooled goat milk sample collected was divided into four lots. One set was used for raw milk analysis and the remaining three were subjected to heat treatment at three different timetemperature combinations namely;72°C for 15 sec, 85°C for 10 min, and 121° C for 15 min. These samples were cooled immediately to 42ºC, inoculated with 0.04 % DVS yogurt culture,incubated at 42º C for 4 hrs, and then stored at 5±2°C for 21 days for storage studies. The analysis was carried out at 7-day intervals. An agar well diffusion assay was performed to assess the inhibitory activity ofWater-soluble peptide extracts (WSPE) of these samples against Bacillus cereus ATCC 10876, Escherichia coli NCIM 2685, Salmonella enterica ATCC 6017, and Staphylococcus aureus ATCC 25923. The milk that was heated to 85°C for 10 min before fermentation showed the highest antibacterial activity against E.coli during the 7th and 14th day of storage, withthe zone of inhibition measuredat 17±0.433mm and 21±0.55mm respectively.Thus, it can be concluded that milk heated at 85°C for 10 min had better antibacterial activity than the other two treatments

<i>In silico</i> approaches illustrate the evolutionary pattern and protein-small molecule interactions of quinolone synthase from <i>Aegle marmelos</i> Correa

<p>Quinolone synthase from <i>Aegle marmelos</i> (AmQNS) is a Rutacean-specific plant type III polyketide synthase that synthesizes quinolone, acridone, and benzalacetone with therapeutic potential. Simple architecture and broad substrate affinity of AmQNS make it as one of the target enzymes to produce novel structural scaffolds. Another unique feature of AmQNS despite its high similarity to acridone forming type III polyketide synthase from <i>Citrus microcarpa</i> is the variation in the product formation. Hence, to explore the characteristic features of AmQNS, an in-depth sequence and structure-based bioinformatics analyses were performed. Our studies indicated that AmQNS and its nearest homologs have evolved by a series of gene duplication events and strong purifying selection pressure constrains them in the evolutionary process. Additionally, some amino acid alterations were identified in the functionally important region(s), which can contribute to the functional divergence of the enzyme. Prediction of favorable amino acid substitutions will be advantageous in the metabolic engineering of AmQNS for the production of novel compounds. Furthermore, comparative modeling and docking studies were utilized to investigate the structural behavior and small molecule interaction pattern of AmQNS. The observations and results reported here are crucial for advancing our understanding of AmQNS’s phylogenetic position, selection pressure, evolvability, interaction pattern and thus providing the foundation for further studies on the structural and reaction mechanism.</p

Multiple stimuli responsive cyclodextrin based smart materials for drug delivery: a review

In this review we discuss about the research advances in multiple stimuli responsive cyclodextrin (CD) based smart materials. CD based systems which are responsive to temperature, pH, enzymes, light, redox etc. along with various applications of these systems in the biomedical area are extensively covered. Current challenges and future perspectives of these smart stimuli-responsive drug delivery systems are also discussed

TEQIP - III Sponsored First International Conference on Innovations and Challenges in Computing, Analytics and Security

This book contains abstracts of the various research papers of the academic &amp; research community presented at the International Conference on Innovations and Challenges in Computing, Analytics and Security (ICICCAS-2020). ICICCAS-2020 has served as a platform for researchers, professionals to meet and exchange ideas on computing, data analytics, and security. The conference has invited papers in seven main tracks of Data Science, Networking Technologies, Sequential, Parallel, Distributed and Cloud Computing, Advances in Software Engineering, Multimedia, Image Processing, and Embedded Systems, Security and Privacy, Special Track (IoT, Smart Technologies and Green Engineering). The Technical and Advisory Committee Members were from various countries that have rich Research and Academic experience. Conference Title: TEQIP - III Sponsored First International Conference on Innovations and Challenges in Computing, Analytics and SecurityConference Acronym: ICICCAS-2020Conference Date: 29-30 July 2020Conference Location: Pondicherry Engineering College, Puducherry – 605014, India (Virtual Mode)Conference Organizer: Department of Computer Science and Engineering, Pondicherry Engineering College, Puducherry, India.Conference Sponsor: TEQIP-III NPIU (A Unit of the Ministry of Human Resource Development, India)

Proceedings of International Web Conference in Civil Engineering for a Sustainable Planet

This proceeding contains articles of the various research ideas of the academic community and practitioners accepted at the "International Web Conference in Civil Engineering for a Sustainable Planet (ICCESP 2021)". ICCESP 2021 is being Organized by the Habilete Learning Solutions, Kollam in Collaboration with American Society of Civil Engineers (ASCE), TKM College of Engineering, Kollam, and Baselios Mathews II College of Engineering, Kollam, Kerala, India. Conference Title: International Web Conference in Civil Engineering for a Sustainable PlanetConference Acronym: ICCESP 2021Conference Date: 05–06 March 2021Conference Location: Online (Virtual Mode)Conference Organizer: Habilete Learning Solutions, Kollam, Kerala, IndiaCollaborators: American Society of Civil Engineers (ASCE), TKM College of Engineering, Kollam, and Baselios Mathews II College of Engineering, Kollam, Kerala, India