51 research outputs found
Novel Biodegradable Polymeric Microparticles Facilitate Scarless Wound Healing by Promoting Re-epithelialization and Inhibiting Fibrosis
Despite decades of research, the goal of achieving scarless wound healing remains elusive. One of the approaches, treatment with polymeric microcarriers, was shown to promote tissue regeneration in various in vitro models of wound healing. The in vivo effects of such an approach are attributed to transferred cells with polymeric microparticles functioning merely as inert scaffolds. We aimed to establish a bioactive biopolymer carrier that would promote would healing and inhibit scar formation in the murine model of deep skin wounds. Here we characterize two candidate types of microparticles based on fibroin/gelatin or spidroin and show that both types increase re-epithelialization rate and inhibit scar formation during skin wound healing. Interestingly, the effects of these microparticles on inflammatory gene expression and cytokine production by macrophages, fibroblasts, and keratinocytes are distinct. Both types of microparticles, as well as their soluble derivatives, fibroin and spidroin, significantly reduced the expression of profibrotic factors Fgf2 and Ctgf in mouse embryonic fibroblasts. However, only fibroin/gelatin microparticles induced transient inflammatory gene expression and cytokine production leading to an influx of inflammatory Ly6C+ myeloid cells to the injection site. The ability of microparticle carriers of equal proregenerative potential to induce inflammatory response may allow their subsequent adaptation to treatment of wounds with different bioburden and fibrotic content
ΠΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π° ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π±ΠΈΠΎΠ³Π΅Π½Π½ΡΡ ΠΊΠ²Π°Π½ΡΠΎΠ²ΡΡ ΡΠΎΡΠ΅ΠΊ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ ΡΡΠ»ΡΡΠΈΠ΄ΠΎΠ² ΡΠ΅ΡΠ΅Π±ΡΠ°, ΠΊΠ°Π΄ΠΌΠΈΡ ΠΈ ΡΠΈΠ½ΠΊΠ° Π΄Π»Ρ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΡ Π±ΠΈΠΎΠ½Π°Π½ΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ½ΡΡ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠ²
The possibility of applying silver, cadmium and zinc sulfide nanoparticles (npAg2S, npCdS and npZnS) obtained using Shewanella oneidensis MR-1 and Bacillus subtilis 168 bacterial cultures for the creation of a new class of polymeric bionanocomposite materials was investigated. Biogenic nanoparticles obtained in aqueous solutions of the corresponding salts in the presence of various types of microorganisms are characterized by the presence of protein molecules on their surface. The molecules composition is determined by the bacterial culture. Proteins stabilize them and allow the nanoparticles to covalently join the active groups of polymeric carriers. Aminated chloromethylated polystyrene microspheres, as well as ion-exchange resins of various types, were used as polymeric matrices. Analysis of interaction with them can be used as a method for studying the properties of biogenic nanoparticles of metal sulfides for subsequent successful selection of a polymeric carrier. The immobilization of biogenic nanoparticles of metal sulfides onto the surface of aminated chloromethylated polystyrene microspheres was found to depend on the level of stability of aqueous nanoparticle suspensions and is determined by the negative charge of biogenic npAg2S, npCdS and npZnS, which suggests covalent binding and the electrostatic interaction of the components in the composition of the polymer bionanocomposite. A comparative analysis of the parameters of nanoparticles depending on the strain used in the biosynthesis was carried out. Analysis of the main physicochemical characteristics of npCdS and npZnS showed that the small size of nanoparticles (npCdS - 5 nm, npZnS - up to 2 nm) and the presence of luminescence peaks at wavelengths less than 400 nm classify them in the blue region of the fluorescence spectrum and identify them as quantum dots. Thus, the possibility of introducing fluorescent quantum dots of nanoparticles of metal sulfides of biogenic origin into various polymeric matrices has been demonstrated, which contributes to the expansion of the horizons for using a new class of nanoparticles to create polymeric bionanocomposites.ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ ΡΡΠ»ΡΡΠΈΠ΄Π° ΡΠ΅ΡΠ΅Π±ΡΠ°, ΠΊΠ°Π΄ΠΌΠΈΡ ΠΈ ΡΠΈΠ½ΠΊΠ° (npAg2S, npCdS ΠΈ npZnS), ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΡ
ΠΊΡΠ»ΡΡΡΡ Shewanella oneidensis MR-1 ΠΈ Bacillus subtilis 168, Π΄Π»Ρ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΊΠ»Π°ΡΡΠ° ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΡ
Π±ΠΈΠΎΠ½Π°Π½ΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ½ΡΡ
ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠ². ΠΠΈΠΎΠ³Π΅Π½Π½ΡΠ΅ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΡ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π² Π²ΠΎΠ΄Π½ΡΡ
ΡΠ°ΡΡΠ²ΠΎΡΠ°Ρ
ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠΈΡ
ΡΠΎΠ»Π΅ΠΉ Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΈΠΏΠΎΠ² ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΡΡ Π½Π°Π»ΠΈΡΠΈΠ΅ΠΌ Π½Π° ΠΈΡ
ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
ΠΌΠΎΠ»Π΅ΠΊΡΠ», ΡΠΎΡΡΠ°Π² ΠΊΠΎΡΠΎΡΡΡ
ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΡΡΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΠΎΠΉ. ΠΠ΅Π»ΠΊΠΈ ΡΡΠ°Π±ΠΈΠ»ΠΈΠ·ΠΈΡΡΡΡ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΡ ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΠΈΠΌ ΠΊΠΎΠ²Π°Π»Π΅Π½ΡΠ½ΠΎ ΠΏΡΠΈΡΠΎΠ΅Π΄ΠΈΠ½ΡΡΡΡΡ ΠΊ Π°ΠΊΡΠΈΠ²Π½ΡΠΌ Π³ΡΡΠΏΠΏΠ°ΠΌ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΡ
Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ. Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΡ
ΠΌΠ°ΡΡΠΈΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ Π°ΠΌΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Ρ
Π»ΠΎΡΠΌ,Π΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½-Π½ΡΠ΅ ΠΏΠΎΠ»ΠΈΡΡΠΈΡΠΎΠ»ΡΠ½ΡΠ΅ ΠΌΠΈΠΊΡΠΎΡΡΠ΅ΡΡ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΈΠΎΠ½ΠΎΠΎΠ±ΠΌΠ΅Π½Π½ΡΠ΅ ΡΠΌΠΎΠ»Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΈΠΏΠΎΠ². ΠΠ½Π°Π»ΠΈΠ· Π²Π·Π°ΠΈΠ»ΡΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Ρ Π½ΠΈΠΌΠΈ Π»ΡΠΆΠ΅Ρ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π»ΡΠ΅ΡΠΎΠ΄Π° ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΠ²ΠΎΠΉΡΡΠ² Π±ΠΈΠΎΠ³Π΅Π½Π½ΡΡ
Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ ΡΡΠ»ΡΡΠΈΠ΄ΠΎΠ² ΠΌΠ΅ΡΠ°Π»Π»ΠΎΠ² Π΄Π»Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅Π³ΠΎ ΡΡΠΏΠ΅ΡΠ½ΠΎΠ³ΠΎ Π²ΡΠ±ΠΎΡΠ° ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ Π½ΠΎΡΠΈΡΠ΅Π»Ρ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΡ Π±ΠΈΠΎΠ³Π΅Π½Π½ΡΡ
Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ ΡΡΠ»ΡΡΠΈΠ΄ΠΎΠ² ΠΌΠ΅ΡΠ°Π»Π»ΠΎΠ² Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ Π°ΠΌΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Ρ
Π»ΠΎΡΠΌΠ΅ΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΏΠΎΠ»ΠΈΡΡΠΈΡΠΎΠ»ΡΠ½ΡΡ
ΠΌΠΈΠΊΡΠΎΡΡΠ΅Ρ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΡΡΠΎΠ²Π½Ρ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΠΈ Π²ΠΎΠ΄Π½ΡΡ
ΡΡΡΠΏΠ΅Π½Π·ΠΈΠΉ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΡΡΡ ΠΎΡΡΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΡΠΌ Π·Π°ΡΡΠ΄ΠΎΠΌ Π±ΠΈΠΎΠ³Π΅Π½Π½ΡΡ
npAg2S, npCdS ΠΈ npZnS, ΡΡΠΎ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅Ρ ΠΊΠΎΠ²Π°Π»Π΅Π½ΡΠ½ΠΎΠ΅ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΠ΅ ΠΈ ΡΠ»Π΅ΠΊΡΡΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² Π² ΡΠΎΡΡΠ°Π²Π΅ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ Π±ΠΈΠΎΠ½Π°Π½ΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ°. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΡΡΠ°ΠΌΠΌΠ°, ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΠΎΠ³ΠΎ Π² Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π΅. ΠΠ½Π°Π»ΠΈΠ· ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ npCdS ΠΈ npZnS ΠΏΠΎΠΊΠ°Π·Π°Π», ΡΡΠΎ Π½Π΅Π±ΠΎΠ»ΡΡΠΈΠ΅ ΡΠ°Π·ΠΌΠ΅ΡΡ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ (npCdS - 5 Π½ΠΌ, npZnS - Π΄ΠΎ 2 Π½ΠΌ) ΠΈ Π½Π°Π»ΠΈΡΠΈΠ΅ Π»ΡΠΌΠΈΠ½Π΅ΡΡΠ΅Π½ΡΠ½ΡΡ
ΠΏΠΈΠΊΠΎΠ² Π½Π° Π΄Π»ΠΈΠ½Π°Ρ
Π²ΠΎΠ»Π½ ΠΌΠ΅Π½Π΅Π΅ 400 Π½ΠΌ, ΡΡΠΎ ΠΎΡΠ½ΠΎΡΠΈΡ ΠΈΡ
ΠΊ ΡΠΈΠ½Π΅ΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠΏΠ΅ΠΊΡΡΠ° ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠΈΠΈ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΊΠ»Π°ΡΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°ΡΡ ΠΈΡ
ΠΊΠ°ΠΊ ΠΊΠ²Π°Π½ΡΠΎΠ²ΡΠ΅ ΡΠΎΡΠΊΠΈ. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, Π±ΡΠ»Π° ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΡΡ
ΠΊΠ²Π°Π½ΡΠΎΠ²ΡΡ
ΡΠΎΡΠ΅ΠΊ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ ΡΡΠ»ΡΡΠΈΠ΄ΠΎΠ² ΠΌΠ΅ΡΠ°Π»Π»ΠΎΠ² Π±ΠΈΠΎΠ³Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΠ΅ ΠΌΠ°ΡΡΠΈΡΡ, ΡΡΠΎ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΡΠ°ΡΡΠΈΡΠ΅Π½ΠΈΡ Π³ΠΎΡΠΈΠ·ΠΎΠ½ΡΠΎΠ² ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΊΠ»Π°ΡΡΠ° Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ Π΄Π»Ρ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΡ
Π±ΠΈΠΎΠ½Π°Π½ΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠΎΠ²
Engineering Escherichia coli for Efficient Aerobic Conversion of Glucose to Malic Acid through the Modified Oxidative TCA Cycle
Malic acid is a versatile building-block chemical that can serve as a precursor of numerous valuable products, including food additives, pharmaceuticals, and biodegradable plastics. Despite the present petrochemical synthesis, malic acid, being an intermediate of the TCA cycle of a variety of living organisms, can also be produced from renewable carbon sources using wild-type and engineered microbial strains. In the current study, Escherichia coli was engineered for efficient aerobic conversion of glucose to malic acid through the modified oxidative TCA cycle resembling that of myco- and cyanobacteria and implying channelling of 2-ketoglutarate towards succinic acid via succinate semialdehyde formation. The formation of succinate semialdehyde was enabled in the core strain MAL 0 (∆ackA-pta, ∆poxB, ∆ldhA, ∆adhE, ∆ptsG, PL-glk, Ptac-galP, ∆aceBAK, ∆glcB) by the expression of Mycobacterium tuberculosis kgd gene. The secretion of malic acid by the strain was ensured, resulting from the deletion of the mdh, maeA, maeB, and mqo genes. The Bacillus subtilis pycA gene was expressed in the strain to allow pyruvate to oxaloacetate conversion. The corresponding recombinant was able to synthesise malic acid from glucose aerobically with a yield of 0.65 mol/mol. The yield was improved by the derepression in the strain of the electron transfer chain and succinate dehydrogenase due to the enforcement of ATP hydrolysis and reached 0.94 mol/mol, amounting to 94% of the theoretical maximum. The implemented strategy offers the potential for the development of highly efficient strains and processes of bio-based malic acid production
Degradation of polycyclic and halogenated aromatic compounds by Rhodococcus and Arthrobacter species
Schmitz A, Fiedler J, Grund E, Denecke B, Eichenlaub R, Gartemann K-H. Degradation of polycyclic and halogenated aromatic compounds by Rhodococcus and Arthrobacter species. In: Debabov VG, Dudnik YV, Danilenko VN, eds. The Biology of Actinomycetes. Proc. of the 9th Intern. Symp. on the Biology of the Actinomycetes (Moscow, July 1994). New York: Allerton Press; 1995: 150-154
Prospects of Applying Biogenic Quantum Dots of Silver, Cadmium and Zinc Sulfides Nanoparticles to Create Polymeric Bionanocomposite Materials
The possibility of applying silver, cadmium and zinc sulfide nanoparticles (npAg2S, npCdS and npZnS) obtained using Shewanella oneidensis MR-1 and Bacillus subtilis 168 bacterial cultures for the creation of a new class of polymeric bionanocomposite materials was investigated. Biogenic nanoparticles obtained in aqueous solutions of the corresponding salts in the presence of various types of microorganisms are characterized by the presence of protein molecules on their surface. The molecules composition is determined by the bacterial culture. Proteins stabilize them and allow the nanoparticles to covalently join the active groups of polymeric carriers. Aminated chloromethylated polystyrene microspheres, as well as ion-exchange resins of various types, were used as polymeric matrices. Analysis of interaction with them can be used as a method for studying the properties of biogenic nanoparticles of metal sulfides for subsequent successful selection of a polymeric carrier. The immobilization of biogenic nanoparticles of metal sulfides onto the surface of aminated chloromethylated polystyrene microspheres was found to depend on the level of stability of aqueous nanoparticle suspensions and is determined by the negative charge of biogenic npAg2S, npCdS and npZnS, which suggests covalent binding and the electrostatic interaction of the components in the composition of the polymer bionanocomposite. A comparative analysis of the parameters of nanoparticles depending on the strain used in the biosynthesis was carried out. Analysis of the main physicochemical characteristics of npCdS and npZnS showed that the small size of nanoparticles (npCdS - 5 nm, npZnS - up to 2 nm) and the presence of luminescence peaks at wavelengths less than 400 nm classify them in the blue region of the fluorescence spectrum and identify them as quantum dots. Thus, the possibility of introducing fluorescent quantum dots of nanoparticles of metal sulfides of biogenic origin into various polymeric matrices has been demonstrated, which contributes to the expansion of the horizons for using a new class of nanoparticles to create polymeric bionanocomposites
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