4,204 research outputs found
Evolutionary history of tall fescue morphotypes inferred from molecular phylogenetics of the Lolium-Festuca species complex
<p>Abstract</p> <p>Background</p> <p>The agriculturally important pasture grass tall fescue (<it>Festuca arundinacea </it>Schreb. syn. <it>Lolium arundinaceum </it>(Schreb.) Darbysh.) is an outbreeding allohexaploid, that may be more accurately described as a species complex consisting of three major (Continental, Mediterranean and rhizomatous) morphotypes. Observation of hybrid infertility in some crossing combinations between morphotypes suggests the possibility of independent origins from different diploid progenitors. This study aims to clarify the evolutionary relationships between each tall fescue morphotype through phylogenetic analysis using two low-copy nuclear genes (encoding plastid acetyl-CoA carboxylase [<it>Acc1</it>] and <it>centroradialis </it>[<it>CEN</it>]), the nuclear ribosomal DNA internal transcribed spacer (rDNA ITS) and the chloroplast DNA (cpDNA) genome-located <it>matK </it>gene. Other taxa within the closely related <it>Lolium</it>-<it>Festuca </it>species complex were also included in the study, to increase understanding of evolutionary processes in a taxonomic group characterised by multiple inter-specific hybridisation events.</p> <p>Results</p> <p>Putative homoeologous sequences from both nuclear genes were obtained from each polyploid species and compared to counterparts from 15 diploid taxa. Phylogenetic reconstruction confirmed <it>F. pratensis </it>and <it>F. arundinacea </it>var. <it>glaucescens </it>as probable progenitors to Continental tall fescue, and these species are also likely to be ancestral to the rhizomatous morphotype. However, these two morphotypes are sufficiently distinct to be located in separate clades based on the ITS-derived data set. All four of the generated data sets suggest independent evolution of the Mediterranean and Continental morphotypes, with minimal affinity between cognate sequence haplotypes. No obvious candidate progenitor species for Mediterranean tall fescues were identified, and only two putative sub-genome-specific haplotypes were identified for this morphotype.</p> <p>Conclusions</p> <p>This study describes the first phylogenetic analysis of the <it>Festuca </it>genus to include representatives of each tall fescue morphotype, and to use low copy nuclear gene-derived sequences to identify putative progenitors of the polyploid species. The demonstration of distinct tall fescue lineages has implications for both taxonomy and molecular breeding strategies, and may facilitate the generation of morphotype and/or sub-genome-specific molecular markers.</p
Silicone encapsulation of thin-film SiOₓ, SiOₓNy and SiC for modern electronic medical implants: a comparative long-term ageing study
Objective. Ensuring the longevity of implantable devices is critical for their clinical usefulness. This is commonly achieved by hermetically sealing the sensitive electronics in a water impermeable housing, however, this method limits miniaturisation. Alternatively, silicone encapsulation has demonstrated long-term protection of implanted thick-film electronic devices. However, much of the current conformal packaging research is focused on more rigid coatings, such as parylene, liquid crystal polymers and novel inorganic layers. Here, we consider the potential of silicone to protect implants using thin-film technology with features 33 times smaller than thick-film counterparts. Approach. Aluminium interdigitated comb structures under plasma-enhanced chemical vapour deposited passivation (SiOx, SiOxNy, SiOxNy + SiC) were encapsulated in medical grade silicones, with a total of six passivation/silicone combinations. Samples were aged in phosphate-buffered saline at 67‰ for up to 694 days under a continuous ±5 V biphasic waveform. Periodic electrochemical impedance spectroscopy measurements monitored for leakage currents and degradation of the metal traces. Fourier-transform infrared spectroscopy, x-ray photoelectron spectroscopy, focused-ion-beam and scanning-electron- microscopy were employed to determine any encapsulation material changes. Main results. No silicone delamination, passivation dissolution, or metal corrosion was observed during ageing. Impedances greater than 100 G were maintained between the aluminium tracks for silicone encapsulation over SiOxNy and SiC passivations. For these samples the only observed failure mode was open-circuit wire bonds. In contrast, progressive hydration of the SiOx caused its resistance to decrease by an order of magnitude. Significance. These results demonstrate silicone encapsulation offers excellent protection to thin-film conducting tracks when combined with appropriate inorganic thin films. This conclusion corresponds to previous reliability studies of silicone encapsulation in aqueous environments, but with a larger sample size. Therefore, we believe silicone encapsulation to be a realistic means of providing long-term protection for the circuits of implanted electronic medical devices
Valence band excitations in V_2O_5
We present a joint theoretical and experimental investigation of the
electronic and optical properties of vanadium pentoxide. Electron energy-loss
spectroscopy in transmission was employed to measure the momentum-dependent
loss function. This in turn was used to derive the optical conductivity, which
is compared to the results of band structure calculations. A good qualitative
and quantitative agreement between the theoretical and the experimental optical
conductivity was observed. The experimentally observed anisotropy of the
optical properties of V_2O_5 could be understood in the light of an analysis of
the theoretical data involving the decomposition of the calculated optical
conductivity into contributions from transitions into selected energy regions
of the conduction band. In addition, based upon a tight binding fit to the band
structure, values are given for the effective V3d_xy-O2p hopping terms and are
compared to the corresponding values for alpha'-NaV_2O_5.Comment: 6 pages (revtex),6 figures (jpg
Discovery of Very High-Energy Gamma-Ray Radiation from the BL Lac 1ES 0806+524
The high-frequency-peaked BL-Lacertae object \objectname{1ES 0806+524}, at
redshift z=0.138, was observed in the very-high-energy (VHE) gamma-ray regime
by VERITAS between November 2006 and April 2008. These data encompass the two-,
and three-telescope commissioning phases, as well as observations with the full
four-telescope array. \objectname{1ES 0806+524} is detected with a statistical
significance of 6.3 standard deviations from 245 excess events. Little or no
measurable variability on monthly time scales is found. The photon spectrum for
the period November 2007 to April 2008 can be characterized by a power law with
photon index between
300 GeV and 700 GeV. The integral flux above 300 GeV is
which corresponds to 1.8% of the Crab Nebula flux. Non contemporaneous
multiwavelength observations are combined with the VHE data to produce a
broadband spectral energy distribution that can be reasonably described using a
synchrotron-self Compton model.Comment: 14 pages, 4 figures, accepted to APJ
Is knife seizure imagery an effective crime deterrent? A multi-method study
The urgency to reduce knife carrying has been recognised by the police services in Scotland and has been addressed by the introduction of various initiatives to deter knife carrying and crime. One tactic includes the sharing of knife seizure images, where pictures of knives recovered by police are shared on media outlets, however, little research has explored whether this is an effective deterrent. The study sought to explore adults' views of the use of knife images as a deterrent to carrying knives utilising a multi-method approach: (1) a cross-sectional online survey (n= 553), and (2) individual, online semi-structured interviews with adults (n = 20). Fearful, worried and angry reactions towards the use of knife seizure images were expressed among adults and concern was raised that the use of such images may reinforce rather than deter knife carrying. Adults expressed concerns that the use of such images served to reinforce negative stereotypes and stigma within communities affected by high knife crime. The implications of these findings emphasise the limitations to the use of knife seizure images as a deterrent against knife carrying and the importance of community involvement in developing preventative and non-discriminatory approaches to tackling knife carrying throughout Scotland
Detection of Extended VHE Gamma Ray Emission from G106.3+2.7 with VERITAS
We report the detection of very-high-energy (VHE) gamma-ray emission from
supernova remnant (SNR) G106.3+2.7. Observations performed in 2008 with the
VERITAS atmospheric Cherenkov gamma-ray telescope resolve extended emission
overlapping the elongated radio SNR. The 7.3 sigma (pre-trials) detection has a
full angular extent of roughly 0.6deg by 0.4deg. Most notably, the centroid of
the VHE emission is centered near the peak of the coincident 12CO (J = 1-0)
emission, 0.4deg away from the pulsar PSR J2229+6114, situated at the northern
end of the SNR. Evidently the current-epoch particles from the pulsar wind
nebula are not participating in the gamma-ray production. The VHE energy
spectrum measured with VERITAS is well characterized by a power law dN/dE =
N_0(E/3 TeV)^{-G} with a differential index of G = 2.29 +/- 0.33stat +/-
0.30sys and a flux of N_0 = (1.15 +/- 0.27stat +/- 0.35sys)x 10^{-13} cm^{-2}
s^{-1} TeV^{-1}. The integral flux above 1 TeV corresponds to ~5 percent of the
steady Crab Nebula emission above the same energy. We describe the observations
and analysis of the object and briefly discuss the implications of the
detection in a multiwavelength context.Comment: 5 pages, 2 figure
VERITAS Upper Limit on the VHE Emission from the Radio Galaxy NGC 1275
The recent detection by the Fermi gamma-ray space telescope of high-energy
gamma-rays from the radio galaxy NGC 1275 makes the observation of the very
high energy (VHE: E > 100 GeV) part of its broadband spectrum particularly
interesting, especially for the understanding of active galactic nuclei (AGN)
with misaligned multi-structured jets. The radio galaxy NGC 1275 was recently
observed by VERITAS at energies above 100 GeV for about 8 hours. No VHE
gamma-ray emission was detected by VERITAS from NGC 1275. A 99% confidence
level upper limit of 2.1% of the Crab Nebula flux level is obtained at the
decorrelation energy of approximately 340 GeV, corresponding to 19% of the
power-law extrapolation of the Fermi Large Area Telescope (LAT) result.Comment: Accepted for publication in ApJ Letter
Towards a framework for critical citizenship education
Increasingly countries around the world are promoting forms of "critical" citizenship in the planned curricula of schools. However, the intended meaning behind this term varies markedly and can range from a set of creative and technical skills under the label "critical thinking" to a desire to encourage engagement, action and political emancipation, often labelled "critical pedagogy". This paper distinguishes these manifestations of the "critical" and, based on an analysis of the prevailing models of critical pedagogy and citizenship education, develops a conceptual framework for analysing and comparing the nature of critical citizenship
Evidence for long-term Gamma-ray and X-ray variability from the unidentified TeV source HESS J0632+057
HESS J0632+057 is one of only two unidentified very-high-energy gamma-ray
sources which appear to be point-like within experimental resolution. It is
possibly associated with the massive Be star MWC 148 and has been suggested to
resemble known TeV binary systems like LS I +61 303 or LS 5039. HESS J0632+057
was observed by VERITAS for 31 hours in 2006, 2008 and 2009. During these
observations, no significant signal in gamma rays with energies above 1 TeV was
detected from the direction of HESS J0632+057. A flux upper limit corresponding
to 1.1% of the flux of the Crab Nebula has been derived from the VERITAS data.
The non-detection by VERITAS excludes with a probability of 99.993% that HESS
J0632+057 is a steady gamma-ray emitter. Contemporaneous X-ray observations
with Swift XRT reveal a factor of 1.8+-0.4 higher flux in the 1-10 keV range
than earlier X-ray observations of HESS J0632+057. The variability in the
gamma-ray and X-ray fluxes supports interpretation of the ob ject as a
gamma-ray emitting binary.Comment: 8 pages, 3 figures, Accepted for publication in The Astrophysical
Journa
- …