High serum osteopontin levels are associated with prevalent fractures and worse lipid profile in post-menopausal women with type 2 diabetes

Purpose: Patients with type 2 diabetes (T2DM) have increased fracture risk. Osteopontin (OPN) is a protein involved in bone remodeling and inflammation. The aim of this study was to evaluate the association of OPN with fracture prevalence and with metabolic parameters in post-menopausal women with T2DM. Methods: Sixty-four post-menopausal women with T2DM (age 67.0 ± 7.8 years, diabetes duration 8.9 ± 6.7 years), enrolled in a previous study, were followed up (3.6 ± 0.9 years). Previous fragility fractures were recorded. The FRAX score (without BMD) was calculated and biochemical parameters (plasma glucose, HbA1c, lipid profile and renal function) were assessed. Serum 25OH-vitamin D, calcium, PTH and OPN were evaluated at baseline. The association between OPN and fracture prevalence at baseline was evaluated by a logistic model. Results: OPN levels were higher in patients with previous fractures (n.25) than in patients without previous fractures at baseline (n.39) (p = 0.006). The odds of having fractures at baseline increased by 6.7 (1.9–31.4, 95% CI, p = 0.007) for each increase of 1 ng/ml in OPN levels, after adjustment for vitamin D and HbA1c levels. Fracture incidence was 4.7%. Higher OPN associated with a decrease in HDL-cholesterol (p = 0.048), after adjustment for age, basal HDL-cholesterol, basal and follow-up HbA1c and follow-up duration. 25OH-vitamin D associated with an increase in FRAX-estimated probability of hip fracture at follow-up (p = 0.029), after adjustment for age, 25OH-vitamin D and time. Conclusions: In post-menopausal women with T2DM, OPN might be a useful marker of fracture and worse lipid profile

First measurements of the Fe oxidation state of spinel inclusions inolivine single crystals from Vulture (Italy) with the in situ synchrotronmicro-Mossbauer technique

The redox state of the Earth's upper mantle (i.e., oxygen fugacity, f(O2)) is a key variable that influences numerous processes occurring at depth like the mobility of volatile species, partial melting, and metasomatism. It is linked to the oxidation state of peridotite rocks, which is normally determined through the available oxythermobarometers after measuring the chemical composition of equilibrated rock-forming minerals and the Fe3+ in redox-sensitive minerals like spinel or garnet. To date, accurate measurements of Fe3+ / Sigma Fe in peridotites have been limited to those peridotites (e.g., harzburgites and lherzolites) for which an oxythermobarometer exists and where spinel (or garnet) crystals can be easily separated and measured by conventional Fe-57 Mossbauer spectroscopy. Wehrlitic rocks have been generally formed by the interaction of a lherzolite with carbonatitic melts and, therefore, have recorded the passage of (metasomatic) fluids at mantle conditions. However, no oxythermobarometer exists to determine their equilibrium f(O2).The aim of this study was to retrieve the f(O2) of the mantle beneath Mt. Vulture volcano (Italy) through the study of a wehrlitic lapillus emitted during the last eruption (similar to 140 kyr ago) that contain olivines with multiple tiny spinel inclusions with sizes < 40 mu m. To our knowledge, the Fe oxidation state of these inclusions has been never determined with the Mossbauer technique due to their small sizes.Here, we present measurements of the Fe3+ / Sigma Fe using in situ synchrotron Mossbauer spectroscopy coupled with chemical and spectroscopic analysis of both host olivine and spinel inclusions.The results show Fe3+ / Sigma Fe ratios of 0.03-0.05 for olivine and 0.40-0.45 for the included spinels, the latter of which appear higher than those reported in literature for mantle spinel harzburgites and lherzolites. Given the evidence of the mantle origin of the trapped spinels, we propose that the high f(O2) (between 0.81 and 1.00 log above the fayalite-magnetite-quartz buffer; FMQ) likely results from the interaction between the pristine spinel lherzolite and a CO2-rich metasomatic agent prior to the spinel entrapment in olivines at mantle depths

Fluctuation-response relation in turbulent systems

We address the problem of measuring time-properties of Response Functions (Green functions) in Gaussian models (Orszag-McLaughin) and strongly non-Gaussian models (shell models for turbulence). We introduce the concept of {\it halving time statistics} to have a statistically stable tool to quantify the time decay of Response Functions and Generalized Response Functions of high order. We show numerically that in shell models for three dimensional turbulence Response Functions are inertial range quantities. This is a strong indication that the invariant measure describing the shell-velocity fluctuations is characterized by short range interactions between neighboring shells

First report of Eocene gadiform fishes from the Trans-Urals (Sverdlovsk and Tyumen regions, Russia)

AbstractThe recent 2014–2017 controlled excavations carried out in quarries of Sverdlovsk and Tyumen regions, Ural and Western Siberia, are contributing to the knowledge of the middle to late Eocene marine fauna from the Turgay Strait, mainly composed of invertebrates and cartilaginous and bony fishes. Here we present a preliminary report of the bony fishes collected during the campaigns carried out in two Eocene Trans-Urals localities. The sediments of these localities were deposited in a large epicontinental marine basin during the middle to late Eocene, when the Turgay Strait connected the Arctic waters to the North and the Peritethys to the South. Most of the bony fish material examined can be assigned to codfishes (Gadiformes) according to morphological comparison with living and fossil taxa. In particular, nearly complete three-dimensionally preserved lower jaws are confidently assigned to the Merlucciidae, whereas isolated vertebrae and bones are referred to as Gadiformes incertae sedis. This report documents the easternmost occurrence of this group of fishes in the northern hemisphere during the early phases of their radiation, revealing their presence into the Turgay Strait before its closure

A Cross-Over in the Enstrophy Decay in Two-Dimensional Turbulence in a Finite Box

The numerical simulation of two-dimensional decaying turbulence in a large but finite box presented in this paper uncovered two physically different regimes of enstrophy decay. During the initial stage, the enstrophy, generated by a random Gaussian initial condition, decays as t^{-gamma} with gamma approximately 0.7-0.8. After that, the flow undergoes a transition to a gas or fluid composed of distinct vortices. Simultaneously, the magnitude of the decay exponent crosses over to gamma approximately 0.4. An exact relation for the total number of vortices, N(t), in terms of the mean circulation of an individual vortex is derived. A theory predicting that N(t) is proportional to t^{-xi} and the magnitudes of exponents gamma=2/5 and xi=4/5 is presented and the possibility of an additional very late-time cross-over to gamma=1/3 and xi=2/3 is also discussed.Comment: 11 pages, 7 figure

Glutathione infusion before primary percutaneous coronary intervention: A randomised controlled pilot study

Objective: In the setting of reperfused ST-elevation myocardial infarction (STEMI), increased production of reactive oxygen species (ROS) contributes to reperfusion injury. Among ROS, hydrogen peroxide (H2O2) showed toxic effects on human cardiomyocytes and may induce microcirculatory impairment. Glutathione (GSH) is a water-soluble tripeptide with a potent oxidant scavenging activity. We hypothesised that the infusion of GSH before acute reoxygenation might counteract the deleterious effects of increased H2O2 generation on myocardium. Methods: Fifty consecutive patients with STEMI, scheduled to undergo primary angioplasty, were randomly assigned, before intervention, to receive an infusion of GSH (2500 mg/25 mL over 10 min), followed by drug administration at the same doses at 24, 48 and 72 hours elapsing time or placebo. Peripheral blood samples were obtained before and at the end of the procedure, as well as after 5 days. H2O2 production, 8-iso-prostaglandin F2α (PGF2α) formation, H2O2 breakdown activity (HBA) and nitric oxide (NO) bioavailability were determined. Serum cardiactroponin T (cTpT) was measured at admission and up to 5 days. Results: Following acute reperfusion, a significant reduction of H2O2 production (p=0.0015) and 8-iso-PGF2α levels (p=0.0003), as well as a significant increase in HBA (p<0.0001)and NO bioavailability (p=0.035), was found in the GSH group as compared with placebo. In treated patients, attenuated production of H2O2 persisted up to 5 days from the index procedure (p=0.009) and these changes was linked to those of the cTpT levels (r=0.41, p=0.023). Conclusion: The prophylactic and prolonged infusion of GSH seems to determine a rapid onset and persistent blunting of H2O2 generation improving myocardial cell survival. Nevertheless, a larger trial, adequately powered for evaluation of clinical endpoints, is ongoing to confirm the current finding

Optimum operating conditions for a real scale ammonia stripping tower: An experimental case

In recent years, several studies focused on developing processes aimed at recovering nutrients such as nitrogen. In this paper, a real plant for ammonia stripping has been tested on a real aqueous waste in order to optimize the process conditions (air flow rate and pH). The results of a finite element mathematical model were compared with the ones of experimental tests. The results showed that 500 Nm3/h was the minimum flow rate necessary to allow reaching ammonia nitrogen removals close to 80% in 9 hours of stripping (reactor operating in semi-batch mode). Moreover, the tests showed the possibility of increasing the pH value by exploiting the alkalinity that characterizes the digestates without the dosage of reagents for basification (e.g. NaOH)