The moduli stack of affine stable toric varieties

Let X be an irreducible affine T-variety. We consider families of affine stable toric T-varieties over X and give a description of the corresponding moduli space as the quotient stack of an open subscheme in a certain toric Hilbert scheme under the action of a torus.Comment: 10 page

Development of Immune-Chromatographic Monoclonal Test-System for the Detection of <i>Yersinia pseudotuberculosis</i>, Serogroup I

Objective of the study was to develop monoclonal immunoassay for the detection of the pseudotuberculosis agent, serogroup I. Materials and methods. Specific components, that were used for immune-chromatographic test-system development were mouse monoclonal antibodies of hybrid cell lines, obtained to lipopolysaccharide antigen of the outer membrane of the pathogen’s «cold» variant (YP-101N2V4, YP-105S5A10); and rabbit anti-species antibodies against murine immunoglobulins. Particles, (30±2) nm in the diameter, were used to prepare colloidal gold-antibody conjugate. Antibody concentration for conjugation was 10-15 % greater than the D580 exit point on the plateau. For the production of immune-chromatographic test-system a set of membranes - MDI Easypack - manufactured by «Advanced Microdevice», India was deployed. Finished conjugate was applied onto the membrane by means of impregnation. Antibodies in the selected quantities were applied onto the analytical and control membranes via Dispensers. Substrates coated with the conjugate and ready-made working membranes were vacuum dried in a heat cabinet. Assembled immune-chromatographic test-systems were cut off 4.5 mm each and tested for specificity and sensitivity. Results and conclusions. Developed has been immune-chromatographic test-system for the detection of pseudotuberculosis pathogen, serogroup I. Utilized have been monoclonal antibodies of the hybrid cell line YP-105C5A10 in colloidal gold conjugate and monoclonal antibodies of the hybrid cell line YP-101H2B4 in the test line. The test-system allows for the detection of Y. pseudotuberculosis strains, serogroup I, at concentrations varying from 500 ths. m.c.·cm-3 (8 of the 11 strains under study) up to 4 million m.c.·cm-3 and does not identify closely related yersinia and heterologous microorganisms in quantities of 100 million m.c.·cm-3

MANUFACTURING OF HYBRIDOMAS-PRODUCERS OF MONOCLONAL ANTIBODIES TO BRUCELLOSIS AGENT ANTIGENS

Objective of study is to prepare hybridomas-producers of monoclonal antibodies to brucellosis agent antigens. Materials and methods. B. abortus, B. melitensis, B. suis strains from the State collection of microorganisms of the 48th Central Research Institute Affiliated Branch and BALB/c mice. Hybridization was performed as described by G.Kohler and C.Milstein in modification by Fazekas De St. and Scheidegger D. The study of specific activity of immune sera, hybridoma supernatants, ascites fluid, and monoclonal antibody preparations was performed using ELISA. Results and conclusions. Obtained and characterized have been hybridomas-producers of monoclonal antibodies to specific antigens of brucellosis agent. They are active and stable antibody producers in the repeated passaging both, in vitro and in vivo. Obtained have also been the ascites fluid and preparations of monoclonal antibodies of brucellosis agent. Carried out has been substantiated selection of antibodies which could provide for the most sensitive ELISA. It is established that the monoclonal antibodies produced by hybridomas 232B6H7, 232G12F7, 233B2C5 in combination with brucellosis rabbit immunoglobulins allow for the identification of microbial cells of type strains of various Brucella species in concentrations ranging from 0,25·106 mc·sm–3 up to 1,0·106 mc·sm–3 and gave negative results with cultures of heterologous microorganisms in the contents of 1,0·108 mc·sm–3. Hybridomas-producers of monoclonal antibodies are planned to be used for the construction and manufacturing of immunodetection test-systems

Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies against Coronavirus SARS-CоV-2

The aim of the work was to obtain and characterize hybridomas producing monoclonal antibodies to antigens of coronavirus SARS‑CoV‑2, promising for the construction of diagnostic immunochemical tests. Materials and methods. Recombinant nucleocapsid and receptor binding fragment of spike protein of SARS‑CoV‑2 were used for immunization of BALB/c mice. Antigens were absorbed on aluminium hydroxide gel and injected subcutaneously to BALB/c mice at a 7-day-interval. Immune splenocytes and myeloma cells SP2/0-Ag14 were fused by polyethylene glycol 1450. Cell cultures producing specific antibodies against nucleocapsid and receptor binding fragment were selected applying indirect ELISA in 96-well plates sensitized by desired antigens. Clones of hybridomas were obtained using the method of limiting dilutions. Production properties were studied through in vitro cultivation in 24-well culture plates. Immune-ascitic fluids were collected during the cultivation of hybrid cells in peritoneal cavities of BALB/c mice. Monoclonal antibodies were purified by affinity chromatography on protein A sepharose sorbent, conjugated with horseradish peroxidase, and tested for the possibility to be used in sandwich ELISA for detection of inactivated SARS‑CoV‑2 coronavirus strain “Isolate B”. Results and discussion. As a result of hybridization and selection of clones, hybridomas producing monoclonal antibodies to nucleocapsid and receptor binding fragment of SARS‑CoV‑2 have been obtained. During the in vitro and in vivo cultivation the clones maintained the consistent proliferative and antibody producing activity. The application of monoclonal antibody 415D12 as a capture one and 411D12 antibody conjugated with horseradish peroxidase as a detector antibody in ELISA allows for identifying SARS‑CoV‑2 coronavirus at a minimum concentration of 1·103 PFU per ml

Development of Immuno-Enzymatic Monoclonal Tests-Systems for the Detection of Glanders and Melioidosis Agents

Objective of the study was the development of immune-enzymatic monoclonal test-kit for detecting glanders and melioidosis agents. Materials and methods. We used microbial cultures and hybrid cell lines obtained from the collection of the «48th Central Research Institute» of the Ministry of Defense of the Russian Federation. Hybridoma cells were incubated in the peritoneal cavity of BALB/c mice. Preparations of glanders and melioidosis monoclonal antibodies were isolated from the ascetic fluids through precipitation with ammonium sulfate and purification by means of ion-exchange chromatography. Specific components of the test-kits were subjected to freeze drying in corresponding protective media. Study of diagnostic properties of the developed test systems was performed using ELISA. Results and conclusions. We have obtained preparations of monoclonal antibodies in vivo, as well as isolated and purified immunoglobulins from ascetic fluids. We also selected the pairs of monoclonal antibodies for manufacturing specific components. Experimental series of immune-enzymatic monoclonal test-systems allowing for specific detection of glanders and melioidosis causative agents in concentrations ranging from 0.5·106 CFU/ml and higher were made. The absence of cross-reactivity with closely related saprophytes and heterologous microorganisms in concentrations of 1,0·108 CFU/ml was shown. Demonstrated was the possibility in principle to differentiate between Burkholderia malleiand Burkholderia pseudomallei using ELISA. Test systems are promising for follow up state registration as medical products for in vitro diagnostics

Manufacturing of Hybridomas-Producers of Monoclonal Anti-Bodies to Tularemia Agent Antigens

Carried out have been two experimental studies on hybridization of mouse myeloma cells and lymphocytes of BALB/c mice immunized with inactivated microbe Francisella tularensis cultures. As a result obtained have been hybridomas-producers of monoclonal antibodies (MAb) specific to the antigens of tularemia agent. Evaluated have been the prospects of its application for the detection of the agent under discussion using enzyme-linked immunoassay. Established is the fact that monoclonal antibodies produced by 31G1F10, 32E5D3, 35B11C8, 36C2F11 hybridomas make it possible to identify microbe cells of various tularemia agent strains when concentrated up to 0.5·106 mc/sm3, and do not interact with cultures of heterologous microorganisms when concentrated to 1.0·108 mc/sm3, which testifies to their specificity. These MAb are planned to be used for the construction of immune-enzyme and immune-chromatographic test-systems designed for tularemia agent detection

Great Thinkers of Antiquity and Sports

Цель настоящего исследования — показать, как происходит формирование личности на примере древнегреческой состязательной культуры, Олимпийских игр. С помощью исторического, биографического, компаративного методов представлено своеобразие агональной культуры древних греков как одной из форм общения людей с богами и высших форм общественной жизни. Доказано, что занятия самосовершенствованием, физическим и духовным, осуществлялось для того, чтобы добиться расположения богов и стать достойным гражданином полиса. На конкретных примерах биографий великих мыслителей древности прослеживается эллинистическая система воспитания и обучения, которая строилась на гармонии интеллектуального и физического развития.The purpose of this study is to show how personality is formed at the dawn of the ancient Greek competitive culture, the Olympic Games. identification of historical, biographical, comparative methods, represented by the peculiar agonal cultures of the ancient Greeks as one of the forms of communication between people and the gods and the highest manifestation of life manifestations. It is proved that self-improvement, physical and spiritual, was carried out in order to achieve the location of the gods and become a worthy citizen of the policy. On specific examples of the biographies of the great thinkers of antiquity, the Hellenistic system of education and training, which was built on the harmony of intellectual and physical development, can be traced

Development of the Immuno-Enzyme Test-System for the Detection of <i>Legionella pheumophila</i>, Serogroup I

Developed is the highly sensitive and specific immuno-enzyme test-system, which is perspective for the detection of L. pneumophilia, serogroup 1. Isolated are the three hybrid cell lines that secrete monoclonal antibodies to specific epitopes of L. pneumophilia, serogroup 1 lipopolysaccharide antigen. Hyper immune rabbit sera, characterized by highly specific activity and specificity, are obtained using lipopolysaccharide antigen

Overview of Design Diagrams for Solid Oxide Fuel Cell and Gas Turbine Hybrid Systems for Combined Heat and Power Generation

В статье представлен всесторонний обзор и классификация возможных компоновочных решений гибридных систем на основе интеграции твердооксидных топливных элементов (ТОТЭ) и газовых турбин (ГТ). Большинство гибридных систем ТОТЭ/ГТ используют в качестве топлива метан в связи с его доступностью и простотой в обращении в отличие от водорода. Важным элементом конструкции установки является подсистема переработки топлива. Системы ТОТЭ/ГТ направляют часть энергии, вырабатываемой топливным элементом, для осуществления процесса парового риформинга метана. Пар производится в анодном отсеке топливного элемента либо альтернативно во внешнем парогенераторе с использованием тепла выходящих с топливного элемента газов. Большинство проектов гибридных систем ТОТЭ/ГТ, представленных в литературе, работают под давлением, установки в них связаны герметично, что позволяет достичь высокой и сверхвысокой энергоэффективности с относительно низкими капитальными затратами. В гибридных системах с атмосферным давлением установки связаны с помощью системы теплообменников, что обеспечивает их независимость и более простой процесс управления. В статье также исследуются более сложные конфигурации ТОТЭ/ГТ с использованием органического цикла Ренкина, с несколькими топливными элементами и газовыми турбинами, с паровой турбиной, системой охлаждения и др. Устройство и характер взаимосвязей между компонентами гибридной системы оказывают значительное влияние на достижения целевых показателей эффективности по результатам численного моделирования предлагаемых схемных решенийThe article presents a comprehensive review and classification of possible layout solutions for hybrid systems based on the integration of solid oxide fuel cells (SOFC) and gas turbines (GT). Most SOFC/GT hybrid systems use methane as a fuel due to its availability and ease of handling, unlike hydrogen. An important element of the plant design is the fuel processing subsystem. SOFC/GT systems direct part of the energy generated by the fuel cell to carry out the methane steam reforming process. Steam is produced in the anode compartment of the fuel cell, or alternatively in an external steam generator, using the heat from the gases leaving the fuel cell. Most of the designs of hybrid SOFC/GT systems presented in the literature are pressurized, the installations in them are hermetically connected, which makes it possible to achieve high and ultra-high energy efficiency with relatively low capital costs. In atmospheric hybrid systems, the units are connected via a system of heat exchangers, which makes them independent and easier to control. The article also explores more complex SOFC/GT configurations using an organic Rankine cycle, multiple fuel cells and gas turbines, with a steam turbine, a cooling system, etc. The design and nature of the relationship between the components of a hybrid system have a significant impact on achieving performance targets based on numerical simulation of the proposed circuit solution

RESEARCH OF ENERGY-EFFICIENT ACTIONS, MADE BY DEVELOPERS IN EKATERINBURG (BY OCTOBER 2016)

This article is a result of a research by USUAA and UrFU students during the period from 01.09.2016 to 31.10.2016 in research more than 70 students and 15 building companies had participated. In the research, information about energy saving measures in business and dwelling buildings in Ekaterinburg was collected and analyzed. The article describes developers’ energy saving measures and compares them with leading foreign methods. Forecasts and recommendations for development in the site of the energy efficient real estate are offered.Данная работа выполнена на основании исследования, проведенного студентами УрГАХУ и УрФУ в период с 01.09.2016 по 31.10.2016, в рамках учебного курса УрГАХУ «Энергосбережение в архитектуре», в котором приняло участие более 70 студентов и 15 строительных компаний. В статье описаны результаты исследования и комплексного анализа энергоэффективных мероприятий, реализуемых застройщиками в г. Екатеринбурге, а также приведено сравнение местных энергоэффективных жилых комплексов с передовыми зарубежными аналогами. Предложены прогнозы и рекомендации для развития в сфере энергоэффективной недвижимости