Enabling in vivo measurements of nanoparticle concentrations with three-dimensional optoacoustic tomography

In this report, we demonstrate the feasibility of using optoacoustic tomography (OAT) to evaluate biodistributions of nanoparticles in animal models. The redistribution of single-walled carbon nanotubes (SWCNTs) was visualized in living mice. Nanoparticle concentrations in harvested organs were measured spectroscopically using the intrinsic optical absorption and fluorescence of SWCNTs. Observed increases in optoacoustic signal brightness in tissues were compared with increases in optical absorption coefficients caused by SWCNT accumulation. The methodology presented in this report can further be extended to calibrate the sensitivity of an optoacoustic imaging system for a range of changes in optical absorption coefficient values at specific locations or organs in a mouse body to enable noninvasive measurements of nanoparticle concentrations in vivo. Additionally, qualitative information provided by OAT and quantitative information obtained ex vivo may provide valuable feedback for advancing methods of quantitative analysis with OAT

Dimensional boundary between the nano- and volume status

There is measured border between nano- and macrostate of substance. This fact is explained with the Debye model for the distribution of the frequencies of phonons in crystals. If the size of crystal such that a phonon with a wave-length bigger than the Debye’s wave-length can not propagate, it acquires the properties of a substance different from the macroscopic properties of the crystal

Transparent dielectrics structure nontermal changes after laser treating

Changes in the molecular structure of polymethyl methacrylate caused by irradiation with laser pulses of different powers were investigated by x-ray diffractometry methods. An analysis was made of the position and half-width of a halo in x-ray diffractograms. Coherent scattering regions and the distributions of the moduli of the interatomic vectors were determined. Action of laser radiation on this transparent dielectric induced molecular structure changes associated with an increase in the molecular order in the amorphous matrix and with the formation of clusters at certain specific laser radiation energies. Similar effects can be observed in glasses

Tribotechnical characteristics of carbon-bearing lubricants

The development of technology for manufacturing nanostructured substances in amounts that could suffice an interlaboratory research is a high priority task for the implementation of nanoproducts. The ultra dispersiblis clusters of synthetic carbon are used as on extra component in polymeric materials. The substances have a high dispersibility and activity of the surface. To study properties of mineral oils, modified ultra dispersiblis clusters of synthetic carbon is of interest. The present activity is devoted to the research of the modifying influence of ultra dispersiblis clusters of synthetic carbon on viscosity of the characteristic of mineral oils and their stability at various temperatures. In the preparation of diamond secondary suspensions not only statistic average sizes of particles should be taken into consideration, but also polydispersity parameters of nanodiamond particle population too

Laser nanothermolysis of human leukemia cells using functionalized plasmonic nanoparticles

In the present work, we present the use of gold nanorods as plasmonic nanoparticles for selective photothermal therapy of human acute (HL-60) and chronicle (K-562) leukemia cells using a near-infrared laser. We improved a published methodology of gold nanorods conjugation to generate high yields of narrow band gold nanorods with an optical absorption centered at 760 nm. The manufactured nanorods were pegylated and conjugated with monoclonal antibody to become non-toxic as biocompatible nanothermolysis agent. Gold nanorods are synthesized and conjugated to CD33 monoclonal antibody. After pegylation, or conjugation with CD33 antibody, gold nanorods were non-toxic to acute and chronic leukemia cells. Our modified gold nanorods CD33 conjugates shown high level of accumulation for both leukemia cell lines, and successful used for nanothermolysis of human leukemia cells in vitro. Each sample was illuminated with 1 or 3 laser shots as for low and for high laser fluence. The radiation was provided by a Quanta Systems q-switched titanium sapphire laser, and the system was designed for maximum sample coverage using non-focused illumination. HL-60 and K-562 cells were treated for 45 min with gold nanorods CD33 conjugated, or with pegylated gold nanorods. The effect of pulsed-laser nanothermolysis for acute and chronic leukemia cells were investigated with cell counting for number of living cells, percentage of cell death and functional parameters such as damage of cell membrane and metabolic activity. Gold nanorods CD33 conjugates significantly increase cell damage for low fluence laser and completely destroyed cancer cells after 3 pulses for low fluence (acute leukemia) and for high fluence laser as for HL-60 (acute) and for K-562 (chronicle) leukemia cells