12 research outputs found
Study of consumer typologies according to consumer attitudes toward high involvement products
DENDROCHRONOLOGICAL RESEARCH OF SCOTS PINE (PINUS SYLVESTRIS L.) GROWING IN VILNIUS AND KAUNAS FOREST PARKS/PAPRASTOSIOS PUŠIES (PINUS SYLVESTRIS L.), AUGANČIOS VILNIAUS IR KAUNO MIESTŲ MIŠKO PARKUOSE, DENDROCHRONOLOGINIAI TYRIMAI/ ДЕНДРОХРОНОЛОГИЧЕСКИЕ ИССЛЕДОВАНИЯ СОСНЫ ОБЫКНОВЕННОЙ (PINUS SYLVESTRIS L.), ПРОИЗРАСТАЮЩЕЙ В ЛЕСНЫХ ПАРКАХ ГОРОДОВ ВИЛЬНЮСА И КАУНАСА
Dendrochronological and other proxy evidence for climatic cooling around 2700 BP and its heliogeophysical forcing.
Picomolar fluorescent probes for compound affinity determination to carbonic anhydrase IX expressed in live cancer cells
Numerous human cancers, especially hypoxic solid tumors, express carbonic anhydrase IX (CAIX), a transmembrane protein with its catalytic domain located in the extracellular space. CAIX acidifies the tumor microenvironment, promotes metastases and invasiveness, and is therefore considered a promising anticancer target. We have designed a series of high affinity and high selectivity fluorescein-labeled compounds targeting CAIX to visualize and quantify CAIX expression in cancer cells. The competitive binding model enabled the determination of common CA inhibitors' dissociation constants for CAIX expressed in exponentially growing cancer cells. All tested sulfonamide compounds bound the proliferating cells with similar affinity as to recombinantly purified CAIX. The probes are applicable for the design of selective drug-like compounds for CAIX and the competition strategy could be applied to other drug targets
Picomolar fluorescent probes for compound affinity determination to carbonic anhydrase IX expressed in live cancer cells
Numerous human cancers, especially hypoxic solid tumors, express carbonic anhydrase IX (CAIX), a transmembrane protein with its catalytic domain located in the extracellular space. CAIX acidifies the tumor microenvironment, promotes metastases and invasiveness, and is therefore considered a promising anticancer target. We have designed a series of high affinity and high selectivity fluorescein-labeled compounds targeting CAIX to visualize and quantify CAIX expression in cancer cells. The competitive binding model enabled the determination of common CA inhibitors’ dissociation constants for CAIX expressed in exponentially growing cancer cells. All tested sulfonamide compounds bound the proliferating cells with similar affinity as to recombinantly purified CAIX. The probes are applicable for the design of selective drug-like compounds for CAIX and the competition strategy could be applied to other drug targets