DBC2 significantly influences cell-cycle, apoptosis, cytoskeleton and membrane-trafficking pathways

The tumor suppressor DBC2 belongs to a previously uncharacterized gene family, RHOBTB (Bric-a-brac, Tramtrack, Broad-complex). The biological roles of RHOBTB proteins, including DBC2, remain unclear. To understand the physiological functions of DBC2, a global approach was applied. Expression of DBC2 was manipulated in HeLa cells and RNA profiling of the cells was performed by microarray analyses. DBC2 was introduced into HeLa cells by a mammalian expression vector with a constitutive promoter. DBC2 knockdown was achieved by RNA interference with small interfering RNA. RNA profiles of these samples were performed by microarray analysis using Affymetrix GeneChip HG-U133A 2.0. The microarray data were analyzed by Microarray Suite 5.0 (MAS 5.0) and Robust Multichip Average (RMA). A list of genes whose expression was significantly altered (p<0.001) was generated and overlaid onto a cellular pathway map in the Ingenuity Systems' Pathway Knowledge Base (Winter'04 Release). Two networks were found to react substantially to DBC2 expression; namely, more than half of participating genes are affected. One of the networks regulates cell growth through cell-cycle control and apoptosis. The other network is related to cytoskeleton and membrane trafficking. Our findings suggest that the biological roles of DBC2 are related directly and/or indirectly to these cellular machineries

Technological Advancements and Elucidation Gadgets for Healthcare Applications: An Exhaustive Methodological Review-Part-I (AI, Big Data, Block Chain, Open-Source Technologies, and Cloud Computing)

In the realm of the emergence and spread of infectious diseases with pandemic potential throughout the history, plenty of pandemics (and epidemics), from the plague to AIDS (1981) and SARS (in 2003) to the bunch of COVID variants, have tormented mankind. Though plenty of technological innovations are overwhelmingly progressing to curb them&mdash;a significant number of such pandemics astounded the world, impacting billions of lives and posing uncovered challenges to healthcare organizations and clinical pathologists globally. In view of addressing these limitations, a critically exhaustive review is performed to signify the prospective role of technological advancements and highlight the implicit problems associated with rendering best quality lifesaving treatments to the patient community. The proposed review work is conducted in two parts. Part 1 is essentially focused upon discussion of advanced technologies akin to artificial intelligence, Big Data, block chain technology, open-source technology, cloud computing, etc. Research works governing applicability of these technologies in solving many uncovered healthcare issues prominently faced by doctors and surgeons in the fields of cardiology, medicine, neurology, orthopaedics, paediatrics, gynaecology, psychiatry, plastic surgery, etc., as well as their role in curtailing the spread of numerous infectious, pathological, neurotic maladies is thrown light off. Boundary conditions and implicitly associated challenges substantiated by remedies coupled with future directions are presented at the end