Дифференциация 2- и 6-изомеров (2-метиламинопропил)бензофурана методом тандемной масс-спектрометрии

Reliable identification of new psychoactive substances of 2-(2-methylaminopropyl) benzofuran and 6-(2-methylaminopropyl)benzofuran is problematic when analyzing by gas chromatography–mass spectrometry method. It found that these two isomers can be reliably differentiated by MS/MS spectra obtained by collision-induced dissociation of their protonated molecules.Достоверная идентификация новых психоактивных соединений 2-(2-метиламинопропил) бензофурана и 6-(2-метиламинопропил)бензофурана проблематична при проведении анализа методом хроматомасс-спектрометрии. Установлено, что эти два изомера могут быть надежно дифференцированы по МС/МС спектрам, полученным в результате диссоциации, индуцируемой соударением, их протонированных молекул

Дифференциация 2- и 6- изомеров (2-метиламинопропил)бензофурана методом тандемной масс-спектрометрии

Reliable identification of new psychoactive substances of 2-(2-methylaminopropyl)benzofuran and 6-(2-methylaminopropyl)benzofuran is problematic when analyzing by gas chromatography–mass spectrometry method. It found that these two isomers can be reliably differentiated by MS/MS spectra obtained by collision-induced dissociation of their protonated molecules.Достоверная идентификация новых психоактивных соединений 2-(2-метиламинопропил)бензофурана и 6-(2-метиламинопропил)бензофурана проблематична при проведении анализа методом хроматомасс-спектрометрии. Установлено, что эти два изомера могут быть надежно дифференцированы по МС/МС спектрам, полученным в результате диссоциации, индуцируемой соударением, их протонированных молекул

Platforms for Promoting a Personal Brand in the Video Production Industry Online

В статье рассматриваются площадки в сети Интернет, с помощью которых специалист в сфере создания видеоконтента может демонстрировать профессиональную деятельность. Поскольку работодатели предпочитают снижать финансовые издержки, в сфере креативных индустрий растет тенденция привлекать фрилансеров для проектной занятости, что приводит к необходимости для специалиста развивать сильный личный бренд. Интернет-платформы — это эффективный и малозатратный инструмент продвижения личного бренда. При выборе площадки учитываются ее преимущества и недостатки, специфика видео-контента, а также особенности поведения целевой аудитории.This article analyzes proper channels through which video content creators can highlight their professional activities. As employers prefer to reduce their financial costs, there is a growing trend in the creative industries to hire freelancers for project employment, that leads to the need for the specialist to build a solid personal brand. Internet platforms are effective and low-cost tool for promoting a personal brand. When choosing a platform, one should take into account its advantages and disadvantages, the specifics of the video content, as well as the characteristics of the target audience’s behavior

Identification and analytical properties of new synthetic cannabimimetics bearing 2,2,3,3-tetramethylcyclopropanecarbonyl moiety

By means of gas chromatography with mass spectrometry detector (GC-MS), liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance spectroscopy (NMR), structure of a series from a novel class of synthetic cannabimimetics bearing 2,2,3,3-tetramethylcyclopropanecarbonyl moiety was established. It was found that this fragment could undergo thermal ring-opening into isomeric structures. The title compounds under action of hydrochloric acid can transform into new compounds which structure is discussed in the paper. The compounds identified could be referred to a new class of 'designer drugs' and are in illegal turnover in Russia and Belarus since the summer of 2011. Analytical data obtained in the paper will make possible reliable identification of such new 'designer drugs' during forensic examination. © 2012 Elsevier Ireland Ltd

Selective Synthesis of Azoloyl NH-1,2,3-Triazoles and Azolyl Diazoketones: Experimental and Computational Insights

Here, we report that the reaction of enaminones, from a class of azole series, with sulfonyl azides leads to a difficult-to-separate mixture of two pairs of compounds: (1) 4-azoloyl-NH-1,2,3-triazoles with sulfonamides and (2) azolyl diazoketones with N-sulfonamidines, as a result of the implementation of two competing reactions. On one hand, the electron-donating methyl or methoxy group in the aryl para-position of arylsulfonyl azides favors the production of NH-1,2,3-triazoles together with sulfonamides. On the other hand, the use of highly electrophilic 4-nitrophenylsulfonyl azide promotes the formation of diazoketones and sulfonamidines. It is shown that the direction of each reaction is not only controlled by the nature of the initial enaminones and sulfonyl azides but also depends on the tested solvent. The problem of removing sulfonamides and amidines from the desired products was solved for the first time using new water-soluble enaminones. Based on the experimental and computational studies, the factors contributing to the selective course of alternative reactions were identified, and methods for the synthesis of azoloyl-NH-1,2,3-triazoles and azolyl diazoketones were developed. Density functional theory (DFT) results have shown that the 1,3-dipolar cycloaddition is totally driven toward one single regioisomer with a high asynchronous bond formation, and the introduction of an electron-deficient group in sulfonyl azides induces faster cycloaddition. Additionally, DFT calculations were used to gain further mechanistic insights on the reaction studied here. © 2022 American Chemical Society. All rights reserved.This work was supported by the Russian Science Foundation (grant no. 18-13-00161, prolongation). A.A.H. thanks the computational resources accessed via Iridis4 provided by the University of Southampton

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses Against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Pediatric solid tumors (PSTs) are life-threatening and can lead to high morbidity and mortality rates in children. Developing novel remedies to treat these tumors, such as glioblastoma multiforme and sarcomas, such as osteosarcoma, and rhabdomyosarcoma, is challenging, despite immense attempts with chemotherapeutic or radiotherapeutic interventions. Soy (Glycine max) and kudzu roots (KR) (Pueraria spp.) are well-known phytoestrogenic botanical sources that contain high amounts of naturally occurring isoflavones. In the present study, we investigated the antioxidant and cytotoxic effects of the extracts of KR and soy molasses (SM) against PSTs. The green extraction of isoflavones from KR and SM was performed using natural deep eutectic solvents. The extracts were subsequently analyzed by high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS), which identified 10 isoflavones in KR extracts and 3 isoflavones in the SM extracts. Antioxidant and cytotoxic activities of KR and SM extracts were assessed against glioblastoma multiforme (A-172), osteosarcoma (HOS), and rhabdomyosarcoma (Rd) cancer cell lines. The KR and SM extracts showed satisfactory cytotoxic effects (IC50) against the cancer cell lines tested, particularly against Rd cancer cell lines, in a dose-dependent manner. Antioxidant activity was found to be significantly (p ≤ 0.05) higher in KR than in SM, which was consistent with the results of the cytotoxic activity observed with KR and SM extracts against glioblastoma and osteosarcoma cells. The total flavonoid content and antioxidant activities of the extracts were remarkably attributed to the isoflavone content in the KR and SM extracts. This study provides experimental evidence that HPLC-ESI-HRMS is a suitable analytical approach to identify isoflavones that exhibit potent antioxidant and anticancer potential against tumor cells, and that KR and SM, containing many isoflavones, can be a potential alternative for health care in the food and pharmaceutical industries. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.This work was supported by the Russian Science Foundation, Grant No. 20-66-47017