Qualitative Indicators of Microbial Cells of Yersinia pestis EV Strain Depending on Their Morphological Traits in Different Temperature Conditions of Manufacturing of Plague Vaccine Preparation

The preference of the (21±1) °C temperature for cultivation of the vaccine strain Yersinia pestis EV in the live plague vaccine biomass production to (27±1) °C is experimentally confirmed. An original interpretation of the mechanism of small cells stability that are formed at (21±1) °C has been proposed. This interpretation stipulates a smaller damaging effect of steam molecules during sublimation in the process of lyophilization

To the Spectral Theory of One-Dimensional Matrix Dirac Operators with Point Matrix Interactions

We investigate one-dimensional (2p 7 2p)-matrix Dirac operators DX,\u3b1 and DX,\u3b2 with point matrix interactions on a discrete set X. Several results of [4] are generalized to the case of (p 7 p)-matrix interactions with p > 1. It is shown that a number of properties of the operators DX,\u3b1 and DX,\u3b2 (self-adjointness, discreteness of the spectrum, etc.) are identical to the corresponding properties of some Jacobi matrices BX,\u3b1 and BX,\u3b2 with (p 7 p)-matrix entries. The relationship found is used to describe these properties as well as conditions of continuity and absolute continuity of the spectra of the operators DX,\u3b1 and DX,\u3b2. Also the non-relativistic limit at the velocity of light c \u2192 1e is studied

Development of New Approaches of Obtaining the Hyperimmune Sera for Production of Medical Immunobiological Preparations

Developed were the new immunization schedules of obtaining plague, brucellar, anthrax, tularemia, cholera, leptospirosis, legionellosis and campilobacteriosis hyperimmune sera based on optimal combinations of specific protein antigenic complexes with immunomodulators, providing high specific immune response in 100 % of animals, significant reduction of immunization terms, material and labor input. Immune sera obtained are high quality biological material to be used for production of different diagnostic immunobiological preparations

Comparative Analysis of Experimental Series of Plague Live Vaccine as for Viability and Thermostability Indices

Comparative analysis of specified indices (viability, thermostability) of plague live vaccine preparations, produced in accordance with different biotechnological production schemes, have been carried out. Shown is that the combination of optimal temperature for Yersinia pestis strain EV biomass propagation (21±1) °C as well as optimal concentration proportion of bacteria and stabilizer in suspension with decreased optical density and volume facilitates effectual moisture removal out of preparation, thus stabilizing microbial cells viability index in the process of storage

Mastering of Technology of Production of Brucellosis Diagnostic Bacteriophages

Presented are technologies of production of brucellosis diagnostic bacteriophages developed on the basis of determination of optimal conditions of the phages reproduction. Stability of the finished product was evaluated using Shuhard control charts