21 research outputs found
Origin of the limited α-amylolysis of debranched maltodextrins crystallized in the A form: A TEM study on model substrates
International audienc
Split crystallization during debranching of maltodextrins at high concentration by isoamylase
International audienc
Morphological and structural features of amylose spherocrystals of A-type
International audienc
Sequence Analysis of the Gene Encoding Amylosucrase from Neisseria polysaccharea and Characterization of the Recombinant Enzyme
The Neisseria polysaccharea gene encoding amylosucrase was subcloned and expressed in Escherichia coli. Sequencing revealed that the deduced amino acid sequence differs significantly from that previously published. Comparison of the sequence with that of enzymes of the α-amylase family predicted a (ÎČ/α)(8)-barrel domain. Six of the eight highly conserved regions in amylolytic enzymes are present in amylosucrase. Among them, four constitute the active site in α-amylases. These sites were also conserved in the sequence of glucosyltransferases and dextransucrases. Nevertheless, the evolutionary tree does not show strong homology between them. The amylosucrase was purified by affinity chromatography between fusion protein glutathione S-transferaseâamylosucrase and glutathione-Sepharose 4B. The pure enzyme linearly elongated some branched chains of glycogen, to an average degree of polymerization of 75
Advanced OPC Mask-3D and Resist-3D modelling
International audienc
Best Focus Shift Mitigation for Extending the Depth of Focus
International audienc