20 research outputs found
Microwave-assisted synthesis and characterizations of nanosized copper ferrite and barium titanate for antimicrobial applications
Science and technology of nanosized bimetallic oxide nanomaterials records the various properties and applications. Especially biomedical applications are viewed in particular due to its nanosized particle size. The present experimentation is reporting the microwave-assisted synthesis of nanosized bimetallic oxides like copper ferrite (CuFe2O4) and barium titanate (BaTiO3) by solid state combustion route using poly (vinyl alcohol) (PVA) as a fuel. The structural and morphological characterizations of the bimetallic oxide nanomaterials are performed out by X-ray diffraction (XRD) and scanning electron micrograph (SEM) tools respectively. These analyses report the crystalline nature of both samples. EDX spectral study is also undertaken to know the existence of different metals in the above-mentioned samples. Bonding nature of the bimetallic oxide samples were readied by Fourier transfer infrared (FT-IR) instrumentation. The study reviewed the varied vibrational modes confirms the phase formation of the samples. UV-Vis and thermal study of these bimetallic oxide samples are also studied extensively to know the thermal and absorption behavior respectively. TGA of both the samples are traced and are showing decomposition at rapid rate. In addition, the maximum absorption peaks due to Ï - Ï* transition confirms the sample formation. Antimicrobial activity of the prepared oxide samples was studied for antibacterial and antifungal behavior. Both samples showing considerable activity against various bacteria and fungi
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Not AvailableCastor has been a recalcitrant crop for in vitro manipulations. To realize the potential of genetic engineering, it is essential to develop a repeatable transformation protocol. In planta transformation has been identified as a method of choice to obtain transgenic lines in crops that are trajectory to in vitro procedures. In the present investigation, we have developed an optimized protocol for realizing transgenic castor plants through Agrobacterium mediated in planta transformation. We have established procedures to ensure better survival of Agrobacterium treated seedlings (T0 ) in transgenic green house as well as for screening T1 progeny plants to identify putative transgenic plants. The optimized factors included: growing the Agro-treated (pricked) seedlings for two days in soilrite and then transferring to soil, treating the two day old seedlings of T1 progeny plants in hygromycin solution @40mg/l for two hours and then transferring the normal looking plants to the soil in transgenic green house. Using the optimized protocol, we have realized 30 transgenic castor plants carrying different gene constructs.Not Availabl
The pheno-genotypic characteristics of infantile acute leukemia in a regional cancer center from South India
Introduction: Acute leukemia (AL) is uncommon in infants, with an annual incidence of 30 per million live births. They have peculiar biological characteristics. Although remarkable progress is seen in treatment of childhood AL, infantile AL remains a resistant subset with a dismally low 4-year survival of 35%.
Objectives: To study the morphological, immunophenotypic, and cytogenetic features of infantile AL. A retrospective study of AL cases in children from birth up to 1 year of age, presenting to the departments of pediatric oncology and hematopathology between January 2010 and April 2015, was conducted.
Results: Thirty-eight cases of infantile AL were included. The mean age at presentation was 10.2 months, and a female preponderance (MâF ratio: 0.65:1) was noted. Hyperleukocytosis (total white cell count >50âĂâ109/mm3) was seen in 13 (39.4%) cases. Immunophenotyping done in 31 cases showed pre-B acute lymphoblastic leukemia (B ALL) in 18 (58%), pre-T ALL in three (9.7%), and acute myeloid leukemia (AML) in 10 (32.3%). CD10 positivity was seen in 12 (57.1%) cases of ALL. Cytogenetic study done in 34 cases showed AML with recurrent genetic abnormalities in four. Mixed lineage leukemia (11q23) abnormality was seen in three cases of ALL. Two cases of AML were associated with trisomy 21. One case with features of AML M7 in a 4-day-old baby turned out to be transient abnormal myelopoiesis on follow-up.
Conclusion: Literature on infantile AL from Indian studies is scarce compared to the available Western literature. Hence an epidemiological study of AL cases was done with review of literature, in an attempt to understand their pheno-genotypic features that influence their behavior. This may help in standardizing the treatment of these rare cases
Mechanistic Insight into the Turn-Off Sensing of Nitroaromatic Compounds Employing Functionalized Polyaniline
[EN] Conducting Polymers (CPs), in recent times have contributed significantly in the detection of High Energy Materials possessing nitro functional groups through fluorescence quenching studies. Camphor sulphuric acid doped polyaniline (C-PANI) is studied with a view to obtain easily processable PANI without pi-stacking for the detection of Picric acid(PA) and p-nitro toluene(pNT) in trace levels. The quenching constant and limit of detection were found to be 2.236x10(-6) M and 6.14x10(-7) M for PA and 1.9447x10(-7) M and 3.57x10(-7) M for pNT, respectively showing a very good sensitivity in detection. Resonance Raman spectroscopy (RRS) showed the involvement of the bipolaronic and benzenoid group in the charge transfer complex formed between the conducting polymer and the analyte. The photoluminescence studies indicated a predominant PET mechanism. The electrochemical studies employing cyclic voltammetry gave higher band gap on comparison with absorption studies. The positive and negative shift in oxidation and reduction potential of polymer-analyte was observed and collaborated with the fluorescence studies to understand the possible fluorescence quenching mechanism from electrochemical approach.The authors greatly acknowledge and thank Prof. G. U. Kulkarni, the Director of Centre for Nano and Soft Matter (CeNS) for providing the facility to work in the centre. Specifically, one of our author, Satish Ashok Ture, expresses his thanks to M/s Premier Explosive Limited for their Financial support (H/A: 4254). We also thank the Spanish Government (RTI2018-100910-B-C41 (MCUI/AEI/FEDER, UE)) and Generalitat Valenciana (PROMETEO2018/024) for their support. Part of this work is carried out under the Gulbarga University-Central University of Karnataka MoU program.Venkatappa, L.; Ture, SA.; Yelamaggad, CV.; Sundaram, VNN.; MartĂnez-Måñez, R.; Abbaraju, V. (2020). Mechanistic Insight into the Turn-Off Sensing of Nitroaromatic Compounds Employing Functionalized Polyaniline. ChemistrySelect. 5(21):6321-6330. https://doi.org/10.1002/slct.202001170S63216330521Rong, M., Lin, L., Song, X., Zhao, T., Zhong, Y., Yan, J., ⊠Chen, X. (2014). A Label-Free Fluorescence Sensing Approach for Selective and Sensitive Detection of 2,4,6-Trinitrophenol (TNP) in Aqueous Solution Using Graphitic Carbon Nitride Nanosheets. Analytical Chemistry, 87(2), 1288-1296. doi:10.1021/ac5039913Shanmugaraju, S., Joshi, S. A., & Mukherjee, P. S. (2011). Fluorescence and visual sensing of nitroaromatic explosives using electron rich discrete fluorophores. Journal of Materials Chemistry, 21(25), 9130. doi:10.1039/c1jm10406cRapp-Wright, H., McEneff, G., Murphy, B., Gamble, S., Morgan, R., Beardah, M., & Barron, L. (2017). 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Not AvailableCastor bean (Ricinus communis L.) is an annual chiefy non-edible oilseed crop of commercial and industrial importance cultivated worldwide. There are
emerging genetic improvement objectives including the need for developing CMS system, resistance to biotic and abiotic stresses, ideal plant types for high-density
planting and mechanical harvesting, and ricin-free castor bean seeds. Conventional
breeding efforts and innovations including hybrid development using two-line system, selecting annual type from perennial nature, incorporating resistance against
diseases, increasing harvest index, etc. have contributed immensely for increasing
the productivity of the crop. However, in the absence of suitable genetic material
with suitable traits, a few of the objectives such as resistance to gray mold disease,
resistance to foliage feeders, ricin-free castor bean, etc. have not been achieved with
the traditional approach. In this background, the success stories that have been witnessed in other crops as well as with the basic information that has been generated
in castor bean suggest that biotechnological approaches employing genomics assisted breeding and genetic engineering strategies have immense potential in the
genetic improvement of castor bean. With this understanding, to create an updated
information, the available literature on various aspects of biotechnological research
such as genetic and genomic resources, genetic engineering tools, and techniques developed in castor bean has been reviewed, and appropriate strategies along with
the future scope are presented that could be adopted for genetic enhancement of
castor bean both for input and output traits.Not Availabl
Characterization of the lactococcal temperate phage TP901-1 and its site-specific integration
Not Available
Not AvailableMajor biotic stresses viz., bacterial blight (BB) and blast and brown plant hopper (BPH) coupled with abiotic stresses like drought stress, significantly affect rice yields. To address this, marker-assisted intercross (IC) breeding involving multiple donors was used to combine three BB resistance genesâxa5, xa13 and Xa21, two blast resistance genesâPi9 and Pi54, two BPH resistance genesâBph20 and Bph21, and four drought tolerant quantitative trait loci (QTL)âqDTY1.1, qDTY2.1, qDTY3.1 and qDTY12.1âin the genetic background of the elite Indian rice cultivar âKrishna Hamsaâ. Three cycles of selective intercrossing followed by selfing coupled with foreground selection and phenotyping for the target traits resulted in the development of 196 introgression lines (ILs) with a myriad of gene/QTL combinations. Based on the phenotypic reaction, the ILs were classified into seven phenotypic classes of resistance/tolerance to the following: (1) BB, blast and droughtâ5 ILs; (2) BB and blastâ10 ILs; (3) BB and droughtâ9 ILs; (4) blast and droughtâ42 ILs; (5) BBâ3 ILs; (6) blastâ84 ILs; and (7) droughtâ43 ILs; none of the ILs were resistant to BPH. Positive phenotypic response (resistance) was observed to both BB and blast in 2 ILs, BB in 9 ILs and blast in 64 ILs despite the absence of corresponding R genes. Inheritance of resistance to BB and/or blast in such ILs could be due to the unknown genes from other parents used in the breeding scheme. Negative phenotypic response (susceptibility) was observed in 67 ILs possessing BB-R genes, 9 ILs with blast-R genes and 9 ILs harboring QTLs for drought tolerance. Complex genic interactions and recombination events due to the involvement of multiple donors explain susceptibility in some of the marker positive ILs. The present investigation successfully demonstrates the possibility of rapid development of multiple stress-tolerant/resistant ILs in the elite cultivar background involving multiple donors through selective intercrossing and stringent phenotyping. The 196 ILs in seven phenotypic classes with myriad of gene/QTL combinations will serve as a useful genetic resource in combining multiple biotic and abiotic stress resistance in future breeding programs.Not Availabl