Finite Element Analysis of Steam Turbine Rotor of 210 MW Power Plant

The steam turbine rotor is subjected to temperature variations in during start up and stop cycle which occurs in short intervals of time. This variation in temperature induces transient thermal stresses in the rotor due to large temperature gradients. The transient stresses occur due to change in the material properties like Young’s modulus of elasticity, coefficient of expansion, thermalconductivity, Poisson’s ratio, specific heat etc. The estimation of transient thermal stresses becomes essential before designing the rotor. The turbine rotor is subjected to thermal as well as mechanical stresses.This paper investigates finite element analysis of 210 MW steam turbine rotorsfor transient thermalloading. The rotor was made of 30Cr1Mo1V.The highpressure turbine model was prepared using CAD software (PRO-E). The model was solved using a 1 Degree slice model as the rotor was Axis-Symmetric about the axis of rotation. The FE modelwas analyzed for transient thermal stresses using Ansys 11. The Von misses stresses were highest at the 11th stage(Groove) at a time step of 12000secs (200 min) during 9 hours cold start up cycle. The transient thermal stresses were found to be much higher than the steady state thermal stresses

AlphaFold2-multimer guided high-accuracy prediction of typical and atypical ATG8-binding motifs

Macroautophagy/autophagy is an intracellular degradation process central to cellular homeostasis and defense against pathogens in eukaryotic cells. Regulation of autophagy relies on hierarchical binding of autophagy cargo receptors and adaptors to ATG8/LC3 protein family members. Interactions with ATG8/LC3 are typically facilitated by a conserved, short linear sequence, referred to as the ATG8/LC3 interacting motif/region (AIM/LIR), present in autophagy adaptors and receptors as well as pathogen virulence factors targeting host autophagy machinery. Since the canonical AIM/LIR sequence can be found in many proteins, identifying functional AIM/LIR motifs has proven challenging. Here, we show that protein modelling using Alphafold-Multimer (AF2-multimer) identifies both canonical and atypical AIM/LIR motifs with a high level of accuracy. AF2-multimer can be modified to detect additional functional AIM/LIR motifs by using protein sequences with mutations in primary AIM/LIR residues. By combining protein modelling data from AF2-multimer with phylogenetic analysis of protein sequences and protein-protein interaction assays, we demonstrate that AF2-multimer predicts the physiologically relevant AIM motif in the ATG8-interacting protein 2 (ATI-2) as well as the previously uncharacterized noncanonical AIM motif in ATG3 from potato (Solanum tuberosum). AF2-multimer also identified the AIM/LIR motifs in pathogen-encoded virulence factors that target ATG8 members in their plant and human hosts, revealing that cross-kingdom ATG8-LIR/AIM associations can also be predicted by AF2-multimer. We conclude that the AF2-guided discovery of autophagy adaptors/receptors will substantially accelerate our understanding of the molecular basis of autophagy in all biological kingdoms

Effect of Polyamines on Storability and Quality of Pomegranate Fruit (Punica granatum L.) Cv. Bhagwa

Pomegranate cv. Bhagawa fruits harvested at adequate stage of maturity were dipped in aqueous solutions containing various concentrations of the polyamines putrescine (1mM, 2mM and 3mM) and spermidine (0.5mM, 1mM and 1.5mM), along with Tween-20 as a surfactant, for 5 minutes. The fruits were then stored at 5°C and 8°C temperature with under 90-95% relative humidity. Polyamine-treated fruits showed reduced chilling-injury, weight loss and respiration rate during storage at these 5°C and 8°C temperatures. An increasing trend in total soluble solids (TSS) content, and a decreasing trend in acidity were found in polyamine-treated fruits during storage at 5°C and 8°C temperature. Maximum reduction in chilling-injury was obtained with putrescine (2mM) at both the storage temperatures. Control fruits stored at 5°C and 8°C temperature rapidly developed chilling-injury developed symptoms of brown discoloration of skin and weight-loss in pomegranate fruits

Nutraceutical Composition of Ber (Zizyphus mauritiana Lamk.) Juice: Effect of Enzyme-Assisted Processing

An investigation was undertaken to study the effect of pre-press maceration treatment with cell-wall degrading enzyme, pectinase, on antioxidant composition of ber juice, during 2011-2012. Enzyme-assisted processing significantly (p<0.05) improved antioxidant composition of ber juice. Ber juice extracted using pectinase had richer nutraceutical composition than in the Control. There was an overall increase of 43% in juice yield, 30% in total phenolics and 37% in total flavonoids with use of pectinase. In vitro total antioxidant activity (AOX) in ber juice was 19.58μmol Trolox/ml in Ferric Reducing Antioxidant Power (FRAP) and 13.44μmol Trolox/ml in Cupric Reducing Antioxidant Capacity (CUPRAC) assay. There was 41-65% increase in total AOX of ber juice extracted with the enzyme overstraight pressed juice. Results indicated that tailoring of the enzyme can yield antioxidant-rich juice products

Antioxidant Composition of Guava (Psidium guajava L.) Beverage Blended with Black-Carrot Juice

An investigation was undertaken to study guava beverage blended with black-carrot juice, during 2011-2012. Enzyme-assisted processing of guava significantly improved the juice yield, total soluble solids, titratable acidity pH, ascorbic acid and sugars by using pectinase enzyme. The blending of guava beverage with black carrot juice significantly improved the functional properties of the guava RTS. Anthocyanin and ascorbic contents of blended guava RTS with black-carrot juice decreased with advancement of storage condition and period

Analytical method development and validation of olmutinib bulk drug as per ICH Q2 guidelines by using RP-HPLC Method

RP-HPLC is fast, simple, sensitive, precise, and reproducible (liquid chromatography) method, developed and validated to analyseolmutinib bulk dosage form. Using C-18 HPLC column separation was carried out. This was maintained at ambient temperature. During separation mobile phase consist of methanol (100 v/v) was delivered at a rate of 1mL/min. Using UV detector analysis was carried out at the wavelength 267.68 nm. RP-HPLC method was validated by using various parameters like, precision, limit of quantitation (LOQ), linearity and robustness. The RP-HPLC method was found to be linear over the concentration ranges from 50-100 μg/mL (r2 =0.999). Retention time for bulk olmutinib was found to be 9.349 min. LOQ of method was 5.8540 μg/mL and LOD 3.0536μg/mL. Thus, the developed RP-HPLC method was found to be robust and rugged which can be applied for the regular analysis of olmutinib in the bulk as well as pharmaceutical dosage form. Keywords: C18, RP-HPLC, Methanol, Olmutini

Spectrophotometric Determination of Olmutinib in Bulk by Area under Curve and First Order Derivative Methods and its Validation as per ICH guidelines

Abstract: A simple, precise and economical UV-spectrophotometric method has been developed for the estimation of Olmutinib from bulk. Two methods were developed First method (A) applied was area under curve (AUC) in which the area was integrated in wavelength from 262-272nm. Second method (B) was first order derivative spectrometric method. In this method absorbance at λmin=256.57nm, λmax=282.83nm and zero cross=267.68nm was measured. Calibration curves were plotted for the method by using instrumental response at selected wavelength and concentration of analyte in the solution. In both the methods, linearity was observed in the concentration range of 2-12µg/ml at the λmax=267.68nm. Accuracy and precision studies were carried out and results were satisfactorily obtained. The drug at each of the 80 %, 100 % and 120 % levels showed good recoveries that is in the range of 98.00 to 99.00% for both methods, hence it could be said that the method was accurate. Limit of detection (LOD) and limit of quantitation (LOQ) were determined for the method. The method was validated as per International Conference on Harmonization. All validation parameters were within the acceptable limit. The developed method was successfully applied to estimate the amount of Olmutinib in pharmaceutical formulation

Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production

Biological pretreatment of lignocellulosic biomass is considered to be energy-efficient and cost-effective. In the present study, sorghum husk was biologically pretreated with a white-rot fungus Phanerochaete chrysosporium (MTCC 4955) under submerged static condition. Ligninolytic enzymes like lignin peroxidase (0.843 U/mL) and manganese peroxidase (0.389 U/mL) played an important role in the biological pretreatment of sorghum husk. Activities of different hydrolytic enzymes such as endoglucanase (57.25 U/mL), exoglucanase (4.76 U/mL), filter paperase (0.580 U/mL), glucoamylase (153.38 U/mL), and xylanase (88.14 U/mL) during biological pretreatment of sorghum husk by P. chrysosporium were evaluated. Enzymatic hydrolysis of untreated sorghum husk and biologically pretreated sorghum husk produced 20.07 and 103.0 mg/g reducing sugars, respectively. This result showed a significant increase in reducing sugar production in the biologically pretreated sorghum husk as compared to its untreated counterpart. Biologically pretreated sorghum husk hydrolysate was further fermented for 48 h using Saccharomyces cerevisiae (KCTC 7296), Pachysolen tannophilus (MTCC 1077), and their co-culture resulting in ethanol yields of 2.113, 1.095, and 2.348%, respectively. The surface characteristics of the substrate were evaluated after the delignification and hydrolysis, using FTIR, XRD, and SEM, confirming the effectiveness of the biological pretreatment process

Polyherbal decoction modulates redox homeostasis during Malachite green induced metabolic stress in Saccharomyces cerevisiae

Malachite green (MG) is aquatic pollutant that induces oxidative stress when comes in contact with the living organisms. In Saccharomyces cerevisiae, MG produces intracellular reactive oxygen species (ROS) and these ROS disturb redox homeostasis and cellular functions leading to early cell death. Exogenous supply of natural antioxidants containing polyherbal decoction may play a crucial role in re-establishment of redox homeostasis by ensuring the cell survival. Exposure of MG to Saccharomyces cerevisiae resulted in a significant decrease (97.8%) in colony forming units (CFU). An Ayurvedic polyherbal formulation ‘Vayasthapana Rasayana’ (VR) which contains natural antioxidants from plants viz. Terminalia chebula, Clitoria ternatea, Boerhaavia diffusa, Centella asiatica, Phyllanthus emblica, Asparagus racemossus and Tinospora cordifolia at 1.0 mg/mL concentration could arrest the oxidative stress during MG exposure. Levels of ROS elevated up to 67.3% on MG exposure; while VR supplementation reduced it by 54.7%. MG induced cellular apoptosis in 38% and necrosis in 27% cells, while VR augmentation reduced it to 8%. Activities of antioxidant enzymes like catalase, superoxide dismutase and glutathione peroxidase in MG exposed cells were induced by 408, 144 and 140%, respectively, whereas, VR supplementation lowered the expressions to 102, 57 and 111%, respectively. Induction in caspases 3/7 activity was also found to be reduced by 65.39% after VR augmentation. Similarly, VR modulated activities of oxido-reductases like lignin peroxidase, laccase, NADH-DCIP reductase and MG reductase. VR supplementation also maintained the MG utilization potential of S. cerevisiae up to 20th exposure cycle which was otherwise arrested to 8th cycle. The treatment also decreased the ROS accumulation and nuclear damage, restoring the cell viability up to 94% and retained normal growth dynamics. Thus, VR supplementation could significantly decrease oxidative stress, enhance cell viability and ultimately protect the dying S. cerevisiae cells during MG exposure

Polyherbal decoction modulates redox homeostasis during Malachite green induced metabolic stress in Saccharomyces cerevisiae

17-26Malachite green (MG) is aquatic pollutant that induces oxidative stress when comes in contact with the living organisms. In Saccharomyces cerevisiae, MG produces intracellular reactive oxygen species (ROS) and these ROS disturb redox homeostasis and cellular functions leading to early cell death. Exogenous supply of natural antioxidants containing polyherbal decoction may play a crucial role in re-establishment of redox homeostasis by ensuring the cell survival. Exposure of MG to Saccharomyces cerevisiae resulted in a significant decrease (97.8%) in colony forming units (CFU). An Ayurvedic polyherbal formulation ‘Vayasthapana Rasayana’ (VR) which contains natural antioxidants from plants viz. Terminalia chebula, Clitoria ternatea, Boerhaavia diffusa, Centella asiatica, Phyllanthus emblica, Asparagus racemossus and Tinospora cordifolia at 1.0 mg/mL concentration could arrest the oxidative stress during MG exposure. Levels of ROS elevated up to 67.3% on MG exposure; while VR supplementation reduced it by 54.7%. MG induced cellular apoptosis in 38% and necrosis in 27% cells, while VR augmentation reduced it to 8%. Activities of antioxidant enzymes like catalase, superoxide dismutase and glutathione peroxidase in MG exposed cells were induced by 408, 144 and 140%, respectively, whereas, VR supplementation lowered the expressions to 102, 57 and 111%, respectively. Induction in caspases 3/7 activity was also found to be reduced by 65.39% after VR augmentation. Similarly, VR modulated activities of oxido-reductases like lignin peroxidase, laccase, NADH-DCIP reductase and MG reductase. VR supplementation also maintained the MG utilization potential of S. cerevisiae up to 20th exposure cycle which was otherwise arrested to 8th cycle. The treatment also decreased the ROS accumulation and nuclear damage, restoring the cell viability up to 94% and retained normal growth dynamics. Thus, VR supplementation could significantly decrease oxidative stress, enhance cell viability and ultimately protect the dying S. cerevisiae cells during MG exposure