96 research outputs found
Cellulase Production by Wild-type Aspergillus niger, Penicillium chrysogenum and Trichoderma harzianum Using Waste Cellulosic Materials
Waste cellulosic materials (corncob, sawdust and
sugarcane pulp) and crystalline cellulose induced
cellulase production in wild strains of Aspergillus niger,
Penicillium chrysogenum and Trichoderma harzianum
isolated from a wood-waste dump in Lagos, Nigeria.
Cellulose-supplemented media gave the maximum
cellulase activity of 0.54, 0.67 and 0.39 units mg Protein-1
for A. niger, P. chrysogenum and T. harzianum
respectively. The maximum enzyme activity for A. niger
was obtained at 36 h of cultivation, while P. chrysogenum
and T. harzianum gave their maximum enzyme activities
at 12 and 60 h respectively. For the cellulosic wastes,
highest enzyme activity was obtained with sawdust where
A. niger, P. chrysogenum and T. harzianum gave the
maximum enzyme activity of 0.30, 0.24 and 0.20 units
mg Protein-1 respectively after 144 h of cultivation. A.
niger recorded the highest enzyme activity with any of the
three cellulosic materials followed by P. chrysogenum. It
thus appears that the use of sawdust presents the best
option for low-cost commercial production of cellulase
using A. niger and P. chrysogenum as discussed herewith
GROWTH AND CELLULASE ACTIVITY OF WILD-TYPE ASPERGILLUS NIGER ANL301 IN DIFFERENT CARBON SOURCES
A wild-type Aspergillus niger (ANL301) isolated from wood-waste in Lagos, Nigeria, produces extracellular
proteins with cellulase (EC 3. 2. 1. 4) activity. Three different carbon sources (Glucose, Cellulose and Sawdust)
influenced the organism’s growth and the production of extracellular cellulase enzymes. Best growth was
obtained with glucose at 72 hours of incubation. The peak mycelia weight of 1.56 mg/ mL obtained with
glucose was about 3 times the maximum weight of 0.58 and 0.49 mg/ mL respectively obtained with cellulose
and sawdust at 96 hours. The peak protein contents of the culture filtrates were 0.02, 0.15 and 0.69 mg/ mL
respectively in the media containing glucose, cellulose and sawdust. There was no significant cellulase activity
in the filtrates from glucose-containing media. The culture filtrates of the organism from cellulose- and
sawdust-containing media yielded significant cellulase activities with maximum values of 105.6 Units /L (at 72
hours for cellulose) and 101.9 Units /L (at 144 hours for sawdust). There is a correlation between the protein
content and cellulase activity of the culture filtrates. Sawdust can serve as a low-cost substrate for cellulase
production by the organism
Properties of Endoglucanase of Penicillium chrysogemum PCL501
Crude extracellular enzyme from a 3-day culture of Penicillium chrysogenum (PCL 501),
in basal medium containing cellulose as the sole carbon source, yielded 0.67 ± 0.03, 19.94 ± 1.30 and
8.50 ± 0.50 units mg protein-1 of 1, 4- â-endoglucanase, â-glucosidase and xylanase activity
respectively. The crude enzyme was subjected to ammonium sulphate precipitation (80% saturation)
and gel filtration. A purification-fold of 7.5 was achieved. Two active fractions of 1, 4 âendoglucanase
(EC 3. 2. 1. 4), which exhibited about the same activity towards carboxymethylcellulose
(CMC), were obtained and pooled for the subsequent analyses. The endoglucanase gave a
Vmax of 10.0 ± 0.4 μmol min-1 mg protein-1 and Km of 11.8 ± 0.4 gL-1 with CMC. The enzyme was
most active at pH of 4.5 – 5.0 and temperature range of 40 – 50 OC. The optimum pH was 4.9 while
the Optimum temperature was 48 OC. Divalent metal ions and EDTA affected the enzyme activity at
2.0 mM concentrations. Mn2+ and Fe2+ had stimulatory effects on the enzyme whereas Mg2+, Cu2+,
Zn2+, Hg2+ and EDTA inhibited the enzyme activity. The effect of Ca2+ was not significant. Over 3-
fold increase in the enzyme activity was recorded with Mn2+. Percentage inhibition of 65.9 and 79.7
respectively was obtained with Hg2+ and EDTA. The organism appears to produce two types of
endoglucanase which differed in their molecular weight but not significantly in their activity.
The enzyme activity was highly stimulated by manganese ion and inhibited by the metal-chelating
agent, EDTA
Isolation of Cellulolytic Microfungi Involved in Wood-Waste Decomposition: Prospects for Enzymatic Hydrolysis of Cellulosic Wastes
Wood-wastes from dump-sites at Okobaba Saw-mills on the western part of the Lagos lagoon were examined for
cellulolytic microorganisms. Cellulolytic microfungi were isolated from the wastes using minimal salt agar medium containing
0.2% (w/v) crystalline cellulose, sugarcane pulp, corn cob or saw-dust as sole carbon/energy source. The colonies of cellulolytic
microfungi which appeared on the plates increased in size and number as the incubation period (days) increased.
Among the fungal isolates were two pathogenic Aspergilli (A flavus and A fumigatus), three different black Aspergilli (herein
designated as A.niger I, A.niger II and A.niger III), Botrytis cinerea, Fusarium species and Penicillium species. Cell-free filtrates
of 7 – day cultures of A.flavus, A.niger I, A.niger II, B. cinerea and P.species grown on the minimal salt broth supplemented with
crystalline cellulose as sole carbon/energy source showed very significant CM–cellulase activity. P. species gave a very high
value that was over 4 times the value for the closest organism, A.niger II. There is a good propect for cellulase production using
the virgin strain of P. species isolated from the wood-wastes
Agro-waste: a potential fermentation substrate for Penicillium chrysogenum
Common agro-wastes found in Lagos, Nigeria (cassava shavings, corncob, sawdust, and sugarcane
pulp) were compared with glucose and lactose as fermentation substrates for Penicillium chrysogenum
PCL501. Cassava shavings significantly (P<0.001) produced the highest amount of mycelia weight (0.43 ±
0.02 mg/ml) than all the other substrates. This was followed by corncob with peak mycelia weight of 0.33 ±
0.02 mg/ml. Peak mycelia weight of 0.27 ± 0.01 mg/ml was equally obtained with glucose and sugarcane pulp
whereas lactose gave a slightly lower peak of 0.25 ± 0.01 mg/ml. Sawdust gave the least mycelia weight of
0.13 ± 0.01 mg/ml. Total sugar content of all the culture media steadily decreased as fungal growth progressed
indicating that the organism utilized carbohydrates for growth and mycelia formation. Cultures containing
cassava shavings and sawdust gave high protein peaks of 0.84 ± 0.05 and 0.65 ± 0.03 mg/ml respectively.
Cultures containing corncob, glucose, lactose and sugarcane pulp yielded lower protein peaks of 0.37 ± 0.02,
0.30 ± 0.02, 0.24 ± 0.02 and 0.18 ± 0.01 mg/ml respectively. The results suggest that cassava shavings,
corncob and sugarcane pulp could serve as cheap fermentation substrates for the growth of the fungus. Of all
the substrates investigated, cassava shavings have the best potential to serve as substrate for fermentation by
Penicillium chrysogenum PCL501.
© 2009 International Formulae Group. All rights reserved
Xylanase production by Aspergillus niger ANL 301 using agro - wastes
Xylanase production by wild-type Aspergillus niger ANL301, newly isolated from wood-waste, was
monitored at 24 h intervals for a period 168 h in media containing different carbon sources. The carbon
sources were oat-spelt xylan (Fluka) and three agro-wastes (sawdust, sugarcane pulp and wheat bran).
Highest xylanase activity of 6.47 units/mL was obtained at 96 h in media containing wheat bran as sole
carbon source. Maximum activity value for the media containing sugarcane pulp was 0.95 units/mL
obtained also at 96 h. Sawdust and oat spelt xylan gave the peak enzyme activities of 0.65 and 0.80
units/mL respectively at 120 h. High protein yield was obtained in media containing the agro-wastes,
with wheat bran giving the highest value of 1.14 mg/mL at 96 h. The maximum specific xylanase
activities were 3.86, 3.37, 5.69, and 9.36 units/ mg protein for sawdust, sugarcane pulp, wheat bran and
oat spelt xylan, respectively. Out of the three agro-wastes used in this study, wheat bran holds greatest
promise for low cost production of the xylanase enzyme
CELLULASE PRODUCTION BY WILD STRAINS OF ASPERGILLUS NIGER, PENICILLIUM CHRYSOGENUM AND TRICHODERMA HARZIANUM GROWN ON WASTE CELLULOSIC MATERIALS.
Waste cellulosic materials (corncob, sawdust and sugarcane pulp) and crystalline cellulose induced cellulase
production in wild strains of Aspergillus niger, Penicillium chrysogenum and Trichoderma harzianum isolated from a
wood-waste dump in Lagos, Nigeria. Cellulose-supplemented media gave the maximum cellulase activity of
-1 0.54, 0.67 and 0.39 units mg Protein for A. niger, P. chrysogenum and T. harzianum respectively. The maximum
enzyme activity for A. niger was obtained at 36 hours of cultivation, while P. chrysogenum and T. harzianum gave
their optimal enzyme activities at 12 and 60 hours respectively. Of the three cellulosic wastes, best enzyme
-1 activity was obtained with sawdust. Maximum enzyme activity of 0.30, 0.24 and 0.20 units mg Protein
respectively was obtained with A. niger, P. chrysogenum and T. harzianum at 144 hours of cultivation using the
substrate. A. niger gave the highest enzyme activity with any of the three cellulosic materials followed by P.
chrysogenum. It thus appears that the use of sawdust presents the best option for low-cost commercial production
of cellulase using A. niger and P. chrysogenum as discussed herewit
Xylanase production by Penicillium chrysogenum (PCL501) fermented on cellulosic wastes
Xylanase production by Penicillium chrysogenum PCL501, newly isolated from wood-wastes, was
monitored at 24 h intervals for a period 168 h in media containing four different carbon sources (oatspelt
xylan, wheat bran, sawdust, and sugarcane pulp). The highest xylanase activity of 6.47 Units mL-1
was obtained at 96 h in media containing wheat bran whereas media containing sugarcane pulp gave a
peak value of 1.39 Units mL-1 at 144 h. Sawdust and xylan gave a peak xylanase activity of 1.35 and 0.79
Units mL-1 respectively at 120 h. Maximum protein released in xylan-containing media was 0.38 mg mL-1.
Higher protein yield was obtained in media containing the agro-wastes, with wheat bran giving the
highest value of 1.14 mg mL-1. The maximum specific xylanase activities were 2.59, 8.52, 16.06, and 9.36
Units mg Protein -1 for sawdust, sugarcane pulp, wheat bran and xylan respectively. Out of the three
agro-wastes used in this study, wheat bran holds the greatest promise for cost-effective production of
the xylanase enzyme. The carbon source is the highest inducer of the enzyme in the fungus
Penicillin Production by Penicillium Chrysogenum PCL 501: Effect of UV Induced Mutation
Penicillin production by Penicillium chrysogenum (PCL501) fermented on glucose, lactose
and four agro-wastes (cassava shavings, corncob, sawdust, and sugarcane pulp) was
monitored with HPLC. The highest amount of penicillin was obtained with sugarcane pulp.
Penicillin yield from a 7-day culture of the fungus was 8.65 ± 0.05, 7.68 ± 0.03, 6.85 ±
0.05, 5.54 ± 0.01, 5.32 ± 0.05 and 2.23 ± 0.02μg/ml respectively on sugarcane pulp,
glucose, cassava shavings, corncob, lactose, and sawdust. Two mutant strains, UVP1 and
UVP2, were obtained by exposing the wild strain (PCL501) to ultraviolet irradiation for 20
and 25 minutes respectively. The 7-day culture of UVP1 and UVP2 on sugarcane pulp
yielded 14.83 ± 0.05 and 14.97 ± 0.05 μg/ml penicillin respectively. This represented over
70% increase in penicillin production over the parent strain. There is a good prospect of
producing cheaper and effective penicillin using the mutant strains of P. chrysogenum an
Plant Waste Hydrolysis by Extracellular Enzymes of Aspergillus niger and Penicillium chrysogenum: Effect of Ammonia Pretreatment
Aspergillus niger (ANL301) and Penicillium chrysogenum (PCL 501) cultured in basal media with cellulose as sole
carbon source yielded extracellular enzymes which partially hydrolyzed sawdust and sugarcane pulp into simple
sugars. Pre-treatment of sawdust by ammonium hydroxide steeping increased the yield of simple sugars. The reducing
sugars released from the pretreated sawdust by the crude enzymes of A. niger (ANL301) and P. chrysogenum
(PCL 501) were 3.58% and 7.02% of the total hydrolysable sugars respectively. This is in contrast to the 0.92% and
1.02% of the total hydrolysable sugars released respectively by the enzymes of A. niger (ANL301) and P. chrysogenum
(PCL 501) from the non-pretreated sawdust. Enzymatic hydrolysis of sugarcane pulp by the crude enzymes
was not significantly affected by ammonia pre-treatment. Reducing sugars released from non-pretreated sugarcane
pulp by the crude enzymes of A. niger (ANL301) and P. chrysogenum (PCL 501) were respectively 4.17% and
5.08% of the total hydrolysable sugars
- …