Effects of ultrasound on Transforming Growth Factor-beta genes in bone cells

Therapeutic ultrasound (US) is a widely used form of biophysical stimulation that is increasingly applied to promote fracture healing. Transforming growth factor-beta (TGF-beta), which is encoded by three related but different genes, is known to play a major part in bone growth and repair. However, the effects of US on the expression of the TGF-beta genes and the physical acoustic mechanisms involved in initiating changes in gene expression in vitro, are not yet known. The present study demonstrates that US had a differential effect on these TGF-beta isoforms in a human osteoblast cell line, with the highest dose eliciting the most pronounced up-regulation of both TGF-beta1 and TGF-beta3 at 1 hour after treatment and thereafter declining. In contrast, US had no effect on TGF-beta2 expression. Fluid streaming rather than thermal effects or cavitation was found to be the most likely explanation for the gene responses observed in vitro

Effects of therapeutic ultrasound on osteoblast gene expression

Ultrasound (US) is commonly used as a physiotherapy aid for a number of types of injury to soft connective tissues and for fracture healing. However, the precise effects of therapeutic US on tissue healing processes are not clearly understood, although they are likely to involve changes in key cellular functions. The present study has therefore examined the effects of several US intensity levels on the activity of two bone-associated proteins, alkaline phosphatase (ALP) and osteopontin (OP) in a human cell line, MG63, using RT-PCR. ALP showed progressively higher expression with increasing US intensities, whereas OP responded differently, showing down-regulation at 120 mW/cm2, the lowest US exposure. OP expression was considerably less affected overall compared with the relative response of ALP to the same US doses. The results show that there is a differential response to therapeutic levels of US, since ALP and OP clearly exhibited gene-specific response profiles. These findings suggest that modifying the parameters of US exposure could be used to improve repair and regeneration processes and enhance the clinical efficacy of implanted biomaterials for tissue engineering

The effects of tides on the water mass mixing and sea ice in the Arctic Ocean

In this study, we use a novel pan-Arctic sea ice-ocean coupled model to examine the effects of tides on sea ice and the mixing of water masses. Two 30 year simulations were performed: one with explicitly resolved tides and the other without any tidal dynamics. We find that the tides are responsible for a ∼15% reduction in the volume of sea ice during the last decade and a redistribution of salinity, with surface salinity in the case with tides being on average ∼1.0–1.8 practical salinity units (PSU) higher than without tides. The ice volume trend in the two simulations also differs: −2.09 × 103 km3/decade without tides and −2.49 × 103 km3/decade with tides, the latter being closer to the trend of −2.58 × 103 km3/decade in the PIOMAS model, which assimilates SST and ice concentration. The three following mechanisms of tidal interaction appear to be significant: (a) strong shear stresses generated by the baroclinic clockwise rotating component of tidal currents in the interior waters; (b) thicker subsurface ice-ocean and bottom boundary layers; and (c) intensification of quasi-steady vertical motions of isopycnals (by ∼50%) through enhanced bottom Ekman pumping and stretching of relative vorticity over rough bottom topography. The combination of these effects leads to entrainment of warm Atlantic Waters into the colder and fresher surface waters, supporting the melting of the overlying ice

Gene expression profiling of bone cells on smooth and rough titanium surfaces

Titanium (Ti) and Ti alloys are widely used as dental and orthopaedic implants, but the effects of the surface characteristics of these materials on the response of cells and target tissues is not well understood. The present study has therefore examined the effects of a rough Ti (RT) and a smooth Ti (ST) surface on human bone cells in vitro. Scanning electron microscopy showed attachment and spreading of cells on both surfaces. Expression profiling using ATLAS™ gene arrays showed marked differences in gene responses after 3 h of culture. A number of osteoblast genes were identified as "roughness response" genes on the basis of changes in expression on the RT compared with the ST surfaces. The surface roughness of Ti was thus found to have a profound effect on the profile of genes expressed by the bone cells, and suggests that improvements in the biological activity and possibly the clinical efficacy of these materials could be achieved by selective regulation of gene expression mediated by controlled modification of Ti surface

Hotspots of dense water cascading in the Arctic Ocean: Implications for the Pacific water pathways

We explore dense water cascading (DWC), a type of bottom‐trapped gravity current, on multidecadal time scales using a pan‐Arctic regional ocean‐ice model. DWC is particularly important in the Arctic Ocean as the main mechanism of ventilation of interior waters when open ocean convection is blocked by strong density stratification. We identify the locations where the most intense DWC events occur and evaluate the associated cross‐shelf mass, heat, and salt fluxes. We find that the modeled locations of cascading agree well with the sparse historical observations and that cascading is the dominant process responsible for cross‐shelf exchange in the boundary layers. Simulated DWC fluxes of 1.3 Sv (1 Sv = 106 m3/s) in the Central Arctic are comparable to Bering Strait inflow, with associated surface and benthic Ekman fluxes of 0.85 and 0.58 Sv. With ice decline, both surface Ekman flux and DWC fluxes are increasing at a rate of 0.023 and 0.0175 Sv/year, respectively. A detailed analysis of specific cascading sites around the Beaufort Gyre and adjacent regions shows that autumn upwelling of warm and saltier Atlantic waters on the shelf and subsequent cooling and mixing of uplifted waters trigger the cascading on the West Chukchi Sea shelf break. Lagrangian particle tracking of low salinity Pacific waters originating at the surface in the Bering Strait shows that these waters are modified by brine rejection and cooling, and through subsequent mixing become dense enough to reach depths of 160–200 m

Direct thrust measurement of a permanent magnet helicon double layer thruster

Direct thrust measurements of a permanent magnet helicon double layer thruster have been made using a pendulum thrust balance and a high sensitivity laser displacement sensor. At the low pressures used (0.08 Pa) an ion beam is detected downstream of the thruster exit, and a maximum thrust force of about 3 mN is measured for argon with an rf input power of about 700 W. The measured thrust is proportional to the upstream plasma density and is in good agreement with the theoretical thrust based on the maximum upstream electron pressure

Direct application of plasmid DNA containing type I interferon transgenes to vaginal mucosa inhibits HSV-2 mediated mortality

The application of naked DNA containing type I interferon (IFN) transgenes is a promising potential therapeutic approach for controlling chronic viral infections. Herein, we detail the application of this approach that has been extensively used to restrain ocular HSV-1 infection, for antagonizing vaginal HSV-2 infection. We show that application of IFN-α1, -α5, and –β transgenes to vaginal mouse lumen 24 hours prior to HSV-2 infection reduces HSV-2 mediated mortality by 2.5 to 3-fold. However, other type I IFN transgenes (IFN- α4, -α5, -α6, and –α9) are non effectual against HSV-2. We further show that the efficacy of IFN-α1 transgene treatment is independent of CD4+ T lymphocytes. However, in mice depleted of CD8+ T lymphocytes, the ability of IFN-α1 transgene treatment to antagonize HSV-2 was lost

Biochemistry Instructors’ Views toward Developing and Assessing Visual Literacy in Their Courses

Biochemistry instructors are inundated with various representations from which to choose to depict biochemical phenomena. Because of the immense amount of visual know-how needed to be an expert biochemist in the 21st century, there have been calls for instructors to develop biochemistry students’ visual literacy. However, visual literacy has multiple aspects, and determining which area to develop can be quite daunting. Therefore, the goals of this study were to determine what visual literacy skills biochemistry instructors deem to be most important and how instructors develop and assess visual literacy skills in their biochemistry courses. In order to address these goals, a needs assessment was administered to a national sample of biochemistry faculty at four-year colleges and universities. Based on the results of the survey, a cluster analysis was conducted to group instructors into categories based on how they intended to develop visual literacy in their courses. A misalignment was found between the visual literacy skills that were most important and how instructors developed visual literacy. In addition, the majority of instructors assumed these skills on assessments rather than explicitly testing them. Implications focus on the need for better measures to assess visual literacy skills directly

Telomerase activity in melanoma and non-melanoma skin cancer

Telomeres are specialized structures consisting of repeat arrays of TTAGGGn located at the ends of chromosomes. They are essential for chromosome stability and, in the majority of normal somatic cells, telomeres shorten with each cell division. Most immortalized cell lines and tumours reactivate telomerase to stabilize the shortening chromosomes. Telomerase activation is regarded as a central step in carcinogenesis and, here, we demonstrate telomerase activation in premalignant skin lesions and also in all forms of skin cancer. Telomerase activation in normal skin was a rare event, and among 16 samples of normal skin (one with a history of chronic sun exposure) 12.5% (2 out of 16) exhibited telomerase activity. One out of 16 (6.25%) benign proliferative lesions, including viral and seborrhoeic wart samples, had telomerase activity. In premalignant actinic keratoses and Bowen's disease, 42% (11 out of 26) of samples exhibited telomerase activity. In the basal cell carcinoma and cutaneous malignant melanoma (CMM) lesions, telomerase was activated in 77% (10 out of 13) and 69% (22 out of 32) respectively. However, only 25% (3 out of 12) of squamous cell carcinomas (SCC) had telomerase activity. With the exception of one SCC sample, telomerase activity in a positive control cell line derived from a fibrosarcoma (HT1080) was not inhibited when mixed with the telomerase-negative SCC or CMM extracts, indicating that, overall, Taq polymerase and telomerase inhibitors were not responsible for the negative results. Mean telomere hybridizing restriction fragment (TRF) analysis was performed in a number of telomerase-positive and -negative samples and, although a broad range of TRF sizes ranging from 3.6 to 17 kb was observed, a relationship between telomerase status and TRF size was not found