Anticathepsin D Antibody-Sepharose Chromatography of Human Cathepsin D

Cathepsin D was isoilated from human tissues by anticatheipsin D antibo:dy-Serpharose 4B .chromatoigraphy. Caithepsin D, rele1ased from the immunoaffinity column formed one precipitin line with specif.ic antibody in immwnod:Lffusion and in immunoe1ectropholl1esis. The isolated proteinase is shown to be prure cathepsin D by actiivity and by inhibition with peipstatin. The quanititative determinati.on o:f cathepsin D in human tissue, taiken by bio.psy from sco,Uotic patients 1rl ivery small amount, is desert.bed. It was demonstrated that cathepsin D from human muscle and human gingival fluid was indistinguishable in immunodiffus ion from the human liver catheps iirl D