Influence of Microclimate in a Barn on Dairy Cows’ Welfare and Production

Microclimate in a barn has a major influence on cows’ health and welfare, as well as on milk production of dairy cows. Extreme values of air temperature and humidity can negatively influence barn conditions, having thus negative effect on cows kept in barns. The research aimed to investigate influence of basic microclimate parameters on a modern dairy farm located in Eastern Croatia. Research period referred to summer season, from 01 June – 31 August 2013. Measurements were carried out in three time intervals (00:00-08:00; 08:00-16:00; 16:00-00:00) for the following parameters: temperature (T), humidity (H) and temperature-humidity index (THI). The results showed that the values of T and THI were higher than optimal values recommended by the scientific literature. Differences between the daily interval for T, H and THI were statistically highly significant (p<0.0001). Furthermore, significant difference (p<0.0001; p<0.05) was determined for all investigated parameters between June and July, and June and August. However, between July and August there was no significant difference. Although measurements were performed on a modern dairy farm equipped with additional cooling by fans, it was not sufficient to create an optimal microclimate environment for dairy cows. Because of that, additional measures need to be undertaken during summer months (for example, installation of water sprinklers) to reduce the air temperature and temperature-humidity index, all with the purpose to increase comfort of dairy cows and to prevent decrease in milk production

Cholelitiasis in an adult patient with mild hereditary spherocytosis – a case report

Hereditary spherocytosis (HS) is an inherited abnormality of the red blood cell, caused by defects in structural membrane proteins. The condition is dominantly inherited in 75% of people. The severity of the disorder is related to the type and amount of membrane disruption, which is genetically determined. A patient who suffers from this disorder is commonly found in a surgical ward when the disease becomes unmanageable by a hematologist. Surgeons encounter complications such as: jaundice, splenomegaly, gallstone sand severe anemia. We present the case of a 66-year-old woman with a history of hereditary spherocytosis who presented at the emergency room for pain in the right upper quadrant, jaundice and anemia and was diagnosed with gallbladder stones and common bile duct obstruction

Osteochondral Grafting: Effect of Graft Alignment, Material Properties, and Articular Geometry

Osteochondral grafting for cartilage lesions is an attractive surgical procedure; however, the clinical results have not always been successful. Surgical recommendations differ with respect to donor site and graft placement technique. No clear biomechanical analysis of these surgical options has been reported. We hypothesized that differences in graft placement, graft biomechanical properties, and graft topography affect cartilage stresses and strains. A finite element model of articular cartilage and meniscus in a normal knee was constructed. The model was used to analyze the magnitude and the distribution of contact stresses, von Mises stresses, and compressive strains in the intact knee, after creation of an 8-mm diameter osteochondral defect, and after osteochondral grafting of the defect. The effects of graft placement, articular surface topography, and biomechanical properties were evaluated. The osteochondral defect generated minimal changes in peak contact stress (3.6 MPa) relative to the intact condition (3.4 MPa) but significantly increased peak von Mises stress (by 110%) and peak compressive strain (by 63%). A perfectly matched graft restored stresses and strains to near intact conditions. Leaving the graft proud by 0.5 mm generated the greatest increase in local stresses (peak contact stresses = 6.7 MPa). Reducing graft stiffness and curvature of articular surface had lesser effects on local stresses. Graft alignment, graft biomechanical properties, and graft topography all affected cartilage stresses and strains. Contact stresses, von Mises stresses, and compressive strains are biomechanical markers for potential tissue damage and cell death. Leaving the graft proud tends to jeopardize the graft by increasing the stresses and strains on the graft. From a biomechanical perspective, the ideal surgical procedure is a perfectly aligned graft with reasonably matched articular cartilage surface from a lower load-bearing region of the knee

Visual engagement with urban street edges: insights using mobile eye-tracking

This study provides empirical insight into the extent to which pedestrians visually engage with urban street edges and how social and spatial factors impact such engagement. This was achieved using mobile eye-tracking. The gaze distribution of 24 study participants was systematically recorded as they carried out everyday tasks on differing streets. The findings demonstrated that street edges are the most visually engaged component of streets; that street edge visual engagement is impacted by everyday social tasks as well as the spatial and physical materiality of edges on differing streets; and that street edges, which attract a lot of visual engagement while undertaking optional tasks, also attract greater amounts of visual engagement while undertaking necessary tasks. These findings offer new insight into urban street edge engagement from the direct perspective of street inhabitants and in doing so provide greater understanding of how street edges are experienced

Nasal obstruction and smell impairment in nasal polyp disease: correlation between objective and subjective parameters

Chronic rhinosinusitis with nasal polyposis (NP) represents an invalidating disorder that causes mainly nasal blockage and loss of smell. The aim of this study is to investigate correlations between individual subjective and objective parameters of stable NP disease. 65 NP patients scored their sinonasal symptoms on a visual analogue scale (VAS) and questionnaires (SNOT-22 and SF-36). Peak nasal inspiratory flow (PNIF) measurement, Sniffinatm Sticks (SS) smell test, blood analysis for eosinophilia, total IgE and culture for Staphylococcus aureus (SA) were performed. VAS scores for nasal blockage correlated with the SNOT-22 and SF-36 scores, which was not observed for VAS of other symptoms. VAS scores for nasal blockage correlated well with PNIF values as well as VAS scores for smell dysfunction and SS results (both p <0.001). NP size correlated with VAS scores for nasal blockage (p <0.01) but not with VAS for other symptoms. NP size showed an inverse correlation with PNIF and SS scores (both p <0.05). Blood eosinophilia correlated with subjective smell reduction (p <0.05). The presence of SA or total IgE levels were not associated with symptoms of NP disease. PNIF and SS are good tools to evaluate symptoms of nasal obstruction and smell reduction in NP disease. Nasal blockage is the only symptom that correlates well with NP size and SNOT-22 scores, whereas smell reduction correlates with blood eosinophili

Dexamethasone-induced apoptosis of freshly isolated human nasal epithelial cells concomitant with abrogation of IL-8 production

SUMMARY Background: Human nasal epithelial cells (hNECs) are the first line of immune defense and are able to produce mediators that recruit, activate and prolong survival of immune cells, among which IL-8 takes an important place. Studies on IL-8 and effects of dexamethasone on hNECs have been hampered by methodological shortcomings. The purpose of the study is to investigate the contribution of freshly isolated hNECs to IL-8 production in chronic rhinosinusitis with nasal polyps (CRSwithNP). Secondly, the effects of dexamethasone treatment on hNEC apoptosis and IL-8 production are investigated. Methodology: hNECs were freshly isolated from nasal polyp tissue and healthy inferior turbinate of NP patients (n=12) and from inferior turbinates of healthy donors (n=19) by protease treatment and two negative selection procedures. hNECs were incubated with IL-1β (10ng/ml), TNFα (10ng/ml) or dexamethasone (10, 100 and 1000 Amicrog/ml). After 24h, IL-8 levels were determined in the supernatants by ELISA. Finally, hNECs were incubated with increasing doses of dexamethasone and stained with trypan-blue and annexin-FITC/PI to study apoptosis. Results: hNECs isolated from nasal turbinates of healthy and NP patients and polyp tissue from NP patients produced similar levels of IL-8. IL-1β induced higher levels of IL-8 production in all types of hNECs without differences between control and NP tissue. Dexamethasone induced apoptosis of hNECs concomitant with abrogation of IL-8 production by hNECs. Conclusions: IL-8 production by human nasal epithelial cells does not differ between NP and healthy tissue under baseline nor stimulatory conditions. Dexamethasone induces apoptosis of hNECs and abrogates IL-8 productio