Additional file 3: of Conceptions of learning factors in postgraduate health sciences master students: a comparative study with non-health science students and between genders

Table S3. Average ± standard deviation scores assigned to each item and to each factor for male and female students and p values of the statistical comparisons between genders for the students of each program using ANOVA (“P value” columns). Statistically significant p values are highlighted with asterisks (*). (PDF 375 kb

Recultivation and cell proliferation analyses of Dupuytren's diseased contracture cords (DDC), palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF), and normal forehand fascia (NFF).

<p>The top panel shows representative images of cells cultured from each tissue type after 7, 14 and 21 days of culture, and the lowest panel shows the analysis of cell proliferation using PCNA immunohistochemistry with two magnification levels.</p

Additional file 1: of Conceptions of learning factors in postgraduate health sciences master students: a comparative study with non-health science students and between genders

Table S1. Factor number 7 related to “Learning as acquisition of professional competences” (PROF) added to the Conceptions of Learning Inventory (COLI) originally developed by Purdie and Hattie. (PDF 174 kb

Average cell viability and functional status according to the WST-1 assay in conjunctival cells incubated with different PGA dilutions for different times.

<p>NC: negative controls for cytotoxicity; PC: positive controls for cytotoxicity.</p

Quantification of the cell number and contents of key ECM components in each tissue type.

<p>NPF: palmar fascia non affected by Dupuytren's disease contracture; DDC: Dupuytren's disease contracture cords; NFF: normal forehand fascia. Values correspond to average ± standard error. Cell number: quantification of the number of cells per area of tissue; SMA: smooth muscle actin. For each variable, the statistical p value for the global comparison using the Kruskal-Wallis test and for the one-to-one comparisons using the Mann-Whitney test are shown.</p><p>Quantification of the cell number and contents of key ECM components in each tissue type.</p

Histochemical and immunohistochemical analysis of Dupuytren's diseased contracture cords (DDC), palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF), and normal forehand fascia (NFF).

<p>3A: Detection of glycoproteins by PAS staining. 3B: Laminin staining by immunohistochemistry. 3C: Quantification of proteoglycans by alcian blue staining. 3D: Aggrecan staining by immunohistochemistry. 3E: Decorin staining by immunohistochemistry. 3F: Versican staining by immunohistochemistry.</p

Microarray expression of ECM components and relevant WNT-pathway genes in the three tissue types analyzed in this work.

<p>Each gene was classified as a fibrillar ECM component (fibers), glycosaminoglycan (GAG), glycoprotein or proteoglycan. NPF: palmar fascia non affected by Dupuytren's disease contracture; DDC: Dupuytren's disease contracture cords; NFF: normal forehand fascia. Statistically significant differences for the U-rank test are labeled with asterisks: in the NPF column, asterisks show statistically significant differences for the comparison of NPF vs. DDC samples; in the DDC column, for the comparison DDC vs. NFF; in the NFF column, for the NFF vs. NPF comparison.</p><p>Microarray expression of ECM components and relevant WNT-pathway genes in the three tissue types analyzed in this work.</p

Average cell viability and functional status according to the LIVE/DEAD assay in conjunctival cells incubated with different PGA dilutions for different times.

<p>NC: negative controls for cytotoxicity; PC: positive controls for cytotoxicity.</p

Analysis of the extracellular matrix fibrillar components of Dupuytren's diseased contracture cords (DDC), palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF), and normal forehand fascia (NFF).

<p>2A: Identification of collagen fibers as determined by picrosirius staining. 2B: Analysis of orientation of collagen fibers as determined by picrosirius staining using polarized microscopy. 2C: Staining of reticular fibers by using the technique of Gomori. 2D: Analysis of elastic fibers as determined by orcein staining.</p

Average cell viability and functional status according to the MTT assay in conjunctival cells incubated with different PGA dilutions for different times.

<p>NC: negative controls for cytotoxicity; PC: positive controls for cytotoxicity.</p