13 research outputs found
Recombinant viral E2 (rE2) interacts with SLAMF3.
<p>(A) Mock and Huh-7+ cells were fixed (in 4% PFA for 10 min) prior to incubation with rE2 for 1 h at 4°C. To measure binding inhibition, cells were incubated with anti-CD81 (JS-81), anti-SLAMF3 (3H) or isotypes for 30 min prior to incubation with rE2 and detection of its binding with anti-E2 antibody by flow cytometry (the mean of two experiments; error bars: SD); (B) Extracted proteins from Huh-7+ and COS-7 were incubated with coated rE2 on an ELISA plate before extensive washing and specific detection of bound CD81 (JS-81) and SLAMF3 (3H) proteins. The optical density (OD) was read at 580 nm and is presented as the mean of two experiments; error bars: SD; (C) Recombinant soluble HCV E2 (rE2) and bovine serum albumin (BSA, used as a control) were coated on nitrocellulose membranes (at the indicated concentrations) prior to incubation with 3/11 E2-specific antibody; (D) rE2 and BSA (5 µg) were coated on nitrocellulose membranes and then incubated with proteins from Huh-7+ cells. Spots were detected using anti-E2 (positive control), anti-SLAMF3 (K12 and HLy9.1.25) and anti-CD81 (JS-81) antibodies; (E) CD81 and SLAMF3 bound to rE2 used at the indicated concentrations were incubated with anti-CD81 (JS-81) and anti-SLAMF3 (HLy9.1.25) antibodies; (F) proteins from native COS-7 cells (top; mock) or SLAMF3-over-expressing COS-7 cells (bottom) were incubated with rE2 and BSA coated on nitrocellulose membranes prior to CD81 and SLAMF3 detection with specific antibodies. One representative experiment from three independent experiments is presented in A, D, E and F.</p
The Expression of the Hepatocyte SLAMF3 (CD229) Receptor Enhances the Hepatitis C Virus Infection
<div><p>Hepatitis C virus (HCV) is a leading cause of cirrhosis and liver cancer worldwide. We recently characterized for the first time the expression of Signaling Lymphocyte Activating Molecule 3 (SLAMF3) in human hepatocytes and here, we report that SLAMF3 interacts with the HCV viral protein E2 and is implicated in HCV entry process. We found a strong correlation between SLAMF3 expression level and hepatocyte susceptibility to HCV infection. The use of specific siRNAs to down-modulate SLAMF3 expression and SLAMF3-blocking antibodies both decreased the hepatocytes susceptibility to HCV infection. Moreover, SLAMF3 over-expression significantly increased susceptibility to HCV infection. Interestingly, experiments with peptides derived from each SLAMF3 domain showed that the first N-terminal extracellular domain is essential for interaction with HCV particles. Finally, we showed that recombinant HCV envelop protein E2 can bind SLAMF3 and that anti-SLAMF3 antibodies inhibited specifically this interaction. Overall, our results revealed that SLAMF3 plays a role during HCV entry, likely by enhancing entry of viral particle within hepatocytes.</p></div
SLAMF3 blockade in human hepatocytes is associated with lower susceptibility to HCV.
<p>(A) SLAMF3 was stained in primary human hepatocytes (PHHs) and cells from the Huh-7 human hepatoma cell line with a specific antibody (HLy9.1.25 clone; grey) and an isotype-matched control (empty). One of four independent experiments is shown. Huh-7 cells were transfected with scrambled control (sc) siRNA or three specific siRNAs (#1, #2 and #3) targeting SLAMF3, prior to infection with HCVcc; siRNA efficiency was checked by quantifying SLAMF3 mRNA (B) and the CD81 expression level (C) by flow cytometry analysis at 48 h post-transfection. Results are presented as the mean ±SD (n = 3). Intracellular viral RNA was quantified at 72 h p.i. (D) and the infection was measured at 72 h p.i. by focus-forming units FFUs counting (E) (as inhibition percent; mean of three independent experiments; error bars: SD. **p<0.01);</p
SLAMF3 over-expression increases hepatocyte permissiveness to HCV.
<p>Huh-7 cells were transfected with empty vector pBud (mock) or human SLAMF3 (to yield Huh-7+ cells) and incubated with HCVcc. (A) SLAMF3 expression was analyzed by flow cytometry (HLy9.1.25 clone) and (B) visualized by confocal microscopy in one out of three experiment. Unshaded histograms show the isotype-matched control (Co) and shaded histograms show SLAMF3 expression). (C, D, E) Infection was assessed at 4 and 72 h p.i. as the number of FFUs and (F and G) by flow cytometry analysis; (C) intracellular E2 (stained red) were measured at 4 h and 72 h p.i. Green staining corresponds to the SLAMF3 expression. One of three representatives independent experiments is shown. (D) The HCV permissiveness of Huh-7 mock and Huh-7+ cells was evaluated in terms of the number of FFUs based on intracellular E2 viral protein staining and (E) fluorescent microscopy analysis (mean of five experiments; error bars: SD; *p<0.05); Infection evaluation by flow cytometry analysis measuring the intracellular viral E2. (F) Infected cells (72 p.i.) were stained for surface expression of SLAMF3 (HLy9.1.25 clone) and permeabilized for intracellular E2 staining. The percentage of positive cells in each cell population is indicated in the corresponding quadrant (one of three representative experiments is shown) and (G) as a mean of three experiments (error bars: SD; *p<0.05).</p
SLAMF3 over-expression increases the HCV infectivity.
<p>Mock and Huh7+ cells were challenged with HCVcc (A, top panel). (A) Supernatants were harvested at 72 h p.i., released viruses quantified by QPCR and equal viral loads from both supernatants were used to infect native Huh-7 cells (A bottom panel). (B) Viral replication was assessed by intracellular viral E2 protein expression measurement by flow cytometry and quantitation of released viral RNA at 72 h p.i. (mean of three independent experiments; error bars: SD *p<0.05, **p<0.01).</p
Hepatocyte SLAMF3-derived peptides inhibit HCV infection.
<p>For peptide competition assays, peptides derived from the first (P1D1 and P2D1), second (P1D2) and third (P1D3) extracellular domains of SLAMF3 and irrelevant peptide (i.p.) used at 0.25 µM were incubated with HCVcc prior to Huh-7 cells infection. (A) The diagram shows SLAMF3's topology and the position of SLAMF3 peptides used to prevent HCV attachment and infection; (B) the inhibitory effect was evaluated by quantification of intracellular viral RNA in infected Huh-7 cells at 72 h p.i. (mean of three experiments; error bars: SD; *p<0.05; **p<0.01, ns: non significant) and (C) in primary human hepatocytes (PHHs) presented as mean of one experiment; (D) Huh7 cells were challenged with HCVcc H77/JFH-1 and the inhibitory effect of SLAMF3-derived peptides was evaluated at 72 h p.i. and presented as FFU values (mean of three experiments; error bars: SD; *p<0.05); (E) The inhibitory effect of SLAMF3-derived peptides was tested on different pseudoparticles harboring envelope glycoproteins derived from HCV genotypes 1a-H77; 2a-HC-J6/CF; 3a-UKN3A1.28 and 4-UKN4.21.16 or from VSV (used as a control to normalize infection assays), (mean of three independent experiments; error bars: SD; *p<0.05, **p<0.01, ns, non-significant).</p
Antibodies against hepatocyte SLAMF3 reduce HCV infection.
<p>(A) Cells from the Huh-7 line were treated with anti-CD81 (JS-81) and/or anti-SLAMF3 (3H, ZY and HLy9.1.25 clones) mAbs prior to HCVcc infection (evaluated by quantification of intracellular vial RNA at 72 p.i.). (B) Cultured primary healthy human hepatocytes PHH were treated with anti-CD81 (JS-81) and/or anti-SLAMF3 (3H) mAbs prior to HCVcc infection (evaluated by quantification of intracellular viral RNA at 72 h p.i.). (Ct) refers to an isotype-matched control (in A and B), all neutralizing antibodies were used at 10 µg/ml; (C) The inhibitory effect of the 3H mAb on the number of FFUs after Huh-7 infection was checked at 72 h p.i. (mean of three independent experiments; error bars, SD * p<0.05, **p<0.01, ***p<0.005, ns: non-significant).</p
Identification of SLAMF3 (CD229) as an Inhibitor of Hepatocellular Carcinoma Cell Proliferation and Tumour Progression
<div><p>Although hepatocellular carcinoma (HCC) is one of the most common malignancies and constitutes the third leading cause of cancer-related deaths, the underlying molecular mechanisms are not fully understood. In the present study, we demonstrate for the first time that hepatocytes express signalling lymphocytic activation molecule family member 3 (SLAMF3/CD229) but not other SLAMF members. We provide evidence to show that SLAMF3 is involved in the control of hepatocyte proliferation and in hepatocellular carcinogenesis. SLAMF3 expression is significantly lower in primary human HCC samples and HCC cell lines than in human healthy primary hepatocytes. In HCC cell lines, the restoration of high levels of SLAMF3 expression inhibited cell proliferation and migration and enhanced apoptosis. Furthermore, SLAMF3 expression was associated with inhibition of HCC xenograft progression in the nude mouse model. The restoration of SLAMF3 expression levels also decreased the phosphorylation of MAPK ERK1/2, JNK and mTOR. In samples from resected HCC patients, SLAMF3 expression levels were significantly lower in tumorous tissues than in peritumoral tissues. Our results identify SLAMF3 as a specific marker of normal hepatocytes and provide evidence for its potential role in the control of proliferation of HCC cells.</p></div
SLAMF3 is expressed by hepatocytes.
<p>(A) SLAMF3 expression was analysed by flow cytometry analysis in HHPHs and in Huh-7, HepG2 and Hep3B human HCC cell lines. SLAMF3 expression in Jurkat lymphocytes was used as a positive control; SLAMF3 staining (in grey) is overlaid by the negative control (in white) and corresponds to one representative of five independent experiments. (B) Western blot analysis of proteins extracted from Huh-7 cells, HepG2 cells or HHPHs, with a mAb (K12) against SLAMF3′s first extracellular domain (D1) or an anti-actin antibody as a control. One of four independent experiments is presented here. (C) Expression of SLAMF3 transcripts in hepatocytes. After reverse transcription, SLAMF3 cDNA was amplified by PCR using specific primers. GAPDH was amplified as a control gene and pure H<sub>2</sub>O was used as a PCR control. The Daudi and Jurkat human lymphocyte cell lines were used as positive controls and the monkey kidney COS-7 cell line was used as a negative control. One of three independent experiments is shown here; (D) SLAMF3 mRNA was assayed by Q-PCR in (i) HHPHs, Huh-7 cells and HepG2 cells, (ii) Daudi B lymphocytes and Jurkat T lymphocytes (positive controls) and (iii) the green monkey kidney COS-7 cell line (a negative control). Results are presented as the mean ± SD (n = 6) ***<i>p<0.005, **p<0.01</i>).</p
Correlation between HCC cell SLAMF3 expression and cell motility.
<p>Cell migration activities in Huh-7 (A) and HepG2 (B) cells overexpressing SLAMF3 and in mock cells were compared in a wound-healing assay. Same areas of culture plate were photographed at the indicated time points. The migratory index corresponds to the percentage of wound closure (estimated using Image J software) and presented as the mean ± SD from three independent experiments with Huh-7 cells (C) (statistical significance: *<i>p<0.05</i> ***<i>p<0.005</i>) and from one experiment for Hep3B cells (D).</p