Evaluación de suelo post-incendio y estrategia de remediación con productos de lombricompostaje

Se evaluó la eficiencia del tratamiento biológico con enmiendas de humus y lixiviado de lombriz roja californiana (Eisenia foetida) sobre las propiedades de un suelo post-incendio. Se realizó un muestreo 48 horas post-incendio y una caracterización inicial. Posteriormente se aplicaron cuatro tratamientos con diferentes porcentajes de humus (H) y lixiviado (L) (testigo, HL5%, HL10% y HL15%) y la siembra de dos especies vegetales, maíz (Zea mays) y tomate (Lycopersicum esculentum). Los cuatro tratamientos fueron evaluados mediante pH, conductividad eléctrica (CE), humedad, capacidad de campo, repelencia WDPT y repelencia MED, así como los atributos funcionales de las dos especies vegetales, durante 90 días. El análisis de varianza (ANOVA) indicó, con un 95% de confiabilidad, que el tratamiento HL15% fue el de mayor eficiencia incrementando la humedad, disminuyendo la repelencia del suelo y mejorando los atributos funcionales de las especies vegetales. Las enmiendas de lombricompostaje favorecen la remediación de suelos impactados por altas temperaturas

Understanding CRISPR/Cas9: A Magnificent Tool for Plant Genome Editing

Nowadays, it is well known that archaea organisms as well as bacteria show an important range of defense mechanisms. Among others, a unique molecular system called CRISPR/Cas (clustered regularly interspaced short palindromic repeats) helps provide protection (adaptive guided immunity) athwart foreign nucleic acids, including plasmids and viral infections. As a typical immune response, CRISPR system is based on the acquisition of genetic records provided by infectious external agents, and in this sense, a high interference upon a new infection is unchained. In relation to plant research, less than 10 years ago, efforts to understand this peculiar mechanism and the possibility of being used in biotechnological processes have been focused on obtaining atavistic changes in different transformable vegetal specimens by inducing selective mutations into a reading frame that may be translated in a given moment (i.e., ORF; open reading frame). In light of the consideration that one common use of ORFs is to assist gene prediction processes, palindromic repeats are mostly based on the directed mutations via nonhomologous end joining. Although it is true that DNA-free editing techniques are now desirable for molecular crop breeding, CRISPR/Cas as a mutational regulatory system in plant biology may offer better complex genome rearrangements