PEMBERDAYAAN IBU NIFAS DALAM MELAKUKAN PERAWATAN BAYI BBLR DI RUMAH DENGAN PENDEKATAN FAMILY CENTERED MATERNITY CARE

Masa nifas dianggap  masa sensitif, ibu  rentan terhadap tekanan psikologis. Pada ibu yang melahirkan anak dengan  berat badan lahir rendah, proses penyesuaian psikologis ibu mempengaruhi perawatan anak dengan berat badan lahir rendah. Berat badan lahir rendah dan bayi baru lahir yang sakit mungkin memerlukan pemantauan tambahan untuk menilai pemulihan, pemberian makan dan berat badan, pemeliharaan suhu, pemberian ASI yang baik, dan kepercayaan diri ibu dalam merawat anak Anda. Perawatan bersalin yang Family Centered Maternity Care (FCMC) adalah perawatan yang berpusat pada keluarga yang memberikan perawatan bagi perempuan dan keluarganya, termasuk kehamilan, kelahiran dan perawatan bayi baru lahir sepanjang kehidupan keluarga. FCMC dilakukan untuk mengoptimalkan kemampuan ibu dalam merawat dirinya dan bayinya. Tujuan dari pengabdian masyarakat untuk membantu para ibu menjadi lebih percaya diri  dalam merawat bayi  dengan berat badan lahir rendah dengan menggunakan pendekatan perawatan ibu yang berpusat pada keluarga. Metode yang digunakan dalam seri ini meliputi pemberdayaan. Memberikan dokumen berupa pemahaman FCMC masa nifas dalam proses perawatan bayi berat lahir rendah di rumah. Melalui hasil yang dicapai setelah berpartisipasi dalam kegiatan peningkatan kesadaran dan diskusi, masyarakat Kelurahan Tanjung Gusta menyadari pentingnya pelayanan ibu yang berpusat pada keluarga

Segregation of the yeast plasmid: Similarities and contrasts with bacterial plasmid partitioning

The high copy yeast plasmid 2μm circle, like the well-studied low copy bacterial plasmids, utilizes two partitioning proteins and a cis-acting \u27centromere\u27-like sequence for its stable propagation. Functionally, though, the protein and DNA constituents of the two partitioning systems are quite distinct. Key events in the yeast and bacterial segregation pathways are plasmid organization, localization, replication, \u27counting\u27 of replicated molecules and their distribution to daughter cells. We suggest that the two systems facilitate these common logistical steps by adapting to the physical, biochemical, and mechanical contexts in which the host chromosomes segregate. © 2004 Elsevier Inc. All rights reserved

Stable propagation of \u27selfish\u27 genetic elements

Extrachromosomal or chromosomally integrated genetic elements are common among prokaryotic and eukaryotic cells. These elements exhibit a variety of \u27selfish\u27 strategies to ensure their replication and propagation during the growth of their host cells. To establish long-term persistence, they have to moderate the degree of selfishness so as not to imperil the fitness of their hosts. Earlier genetic and biochemical studies together with more recent cell biological investigations have revealed details of the partitioning mechanisms employed by low copy bacterial plasmids. At least some bacterial chromosomes also appear to rely on similar mechanisms for their own segregation. The 2 μm plasmid of Saccharomyces cerevisiae and related yeast plasmids provide models for optimized eukaryotic selfish DNA elements. Selfish DNA elements exploit the genetic endowments of their hosts without imposing an undue metabolic burden on them. The partitioning systems of these plasmids appear to make use of a molecular trick by which the plasmids feed into the segregation pathway established for the host chromosomes

Enzymatic characterization of RV <i>in vitro</i> and <i>in vivo</i> immunostimulatory RNAs.

<p>(A) 2’-O-methyltransferase (2’OMT), antarctic phosphatase (AP), and 5’ capping (CE) reactions were set up using <i>in vitro</i> ssRNA. (B) Similar enzymatic reactions were carried out with the <i>in vivo</i> 6 hr large RNA sample as in A. (A and B) Treated RNAs were purified and WT MEFs were transfected with 500 ng/well of the indicated RNA sample. (C) WT MEFs were mock-transfected or transfected in triplicate with 500 ng/well of polyI:C as negative and positive controls, respectively. (A–C) Approximately 21 hours later, mouse IFN-beta ELISA was used to measure the concentration of secreted IFN-beta protein in the cell media. Bars show the average IFN-beta concentration plus standard deviation. Data shown are from one representative experiment out of three. * <i>P</i> < 0.01 (unpaired Student’s <i>t</i>-test).</p

Role of the RLRs in detection of RV <i>in vitro</i> and <i>in vivo</i> immunostimulatory RNAs.

<p>(A) Matched RIG-I wild type (WT) and knock-out (KO) MEFs were mock-transfected or transfected with 500 ng/well of <i>in vitro</i> ssRNA, <i>in vivo</i> 6 hr large RNAs, <i>in vivo</i> 1 hr large RNAs, or polyI:C. (B) Matched MDA5 WT and KO MEFs were transfected exactly as in A. (C) Matched MAVS WT and KO MEFs were transfected exactly as in A. (A–C) Approximately 21 hours later, mouse IFN-beta ELISA was used to measure the concentration of secreted IFN-beta protein in the cell media. Bars show the average IFN-beta concentration plus standard deviation. ND, not detected.</p

Lack of detectable dsRNA in and isolation of immunostimulatory RNAs from RV-infected cells.

<p>(A) MA104 cells in chamber slides were mock-infected, infected with RRV at an estimated MOI of 10, or transfected with 1 μg/well of polyI:C and fixed after a 6-hour (hr) incubation. J2 monoclonal antibody was used to detect dsRNA (red) and NSP5 polyclonal antibody was used to visualize infected cells (green). DAPI was used to stain cell nuclei (blue). (B) Wild type murine embryonic fibroblasts (MEFs) were mock-transfected or transfected with 500 ng/well of <i>in vivo</i> 6 hr large RNAs, <i>in vivo</i> 1 hr large RNAs, or polyI:C. Approximately 21 hours later, mouse IFN-beta ELISA was used to measure the concentration of secreted IFN-beta protein in the cell media. Bars show the average IFN-beta concentration plus standard deviation. ND, not detected.</p

Mutations in a partitioning protein and altered chromatin structure at the partitioning locus prevent cohesin recruitment by the Saccharomyces cerevisiae plasmid and cause plasmid missegregation

The 2μm circle is a highly persistent selfish DNA element resident in the Saccharomyces cerevisiae nucleus whose stability approaches that of the chromosomes. The plasmid partitioning system, consisting of two plasmid-encoded proteins, Rep1p and Rep2p, and a cis-acting locus, STB, apparently feeds into the chromosome segregation pathway. The Rep proteins assist the recruitment of the yeast cohesin complex to STB during the S phase, presumably to apportion the replicated plasmid molecules equally to daughter cells. The DNA-protein and protein-protein interactions of the partitioning system, as well as the chromatin organization at STB, are important for cohesin recruitment. Rep1p variants that are incompetent in binding to Rep2p, STB, or both fail to assist the assembly of the cohesin complex at STB and are nonfunctional in plasmid maintenance. Preventing the cohesin-STB association without impeding Rep1p-Rep2p-STB interactions also causes plasmid missegregation. During the yeast cell cycle, the Rep1p and Rep2p proteins are expelled from STB during a short interval between the late G1 and early S phases. This dissociation and reassociation event ensures that cohesin loading at STB is replication dependent and is coordinated with chromosomal cohesin recruitment. In an rsc2Δ yeast strain lacking a specific chromatin remodeling complex and exhibiting a high degree of plasmid loss, neither Rep1p nor the cohesin complex can be recruited to STB. The phenotypes of the Rep1p mutations and of the rsc2Δ mutant are consistent with the role of cohesin in plasmid partitioning being analogous to that in chromosome partitioning

SARS-CoV-2 vaccination modelling for safe surgery to save lives: data from an international prospective cohort study

Background: Preoperative SARS-CoV-2 vaccination could support safer elective surgery. Vaccine numbers are limited so this study aimed to inform their prioritization by modelling. Methods: The primary outcome was the number needed to vaccinate (NNV) to prevent one COVID-19-related death in 1 year. NNVs were based on postoperative SARS-CoV-2 rates and mortality in an international cohort study (surgical patients), and community SARS-CoV-2 incidence and case fatality data (general population). NNV estimates were stratified by age (18-49, 50-69, 70 or more years) and type of surgery. Best- and worst-case scenarios were used to describe uncertainty. Results: NNVs were more favourable in surgical patients than the general population. The most favourable NNVs were in patients aged 70 years or more needing cancer surgery (351; best case 196, worst case 816) or non-cancer surgery (733; best case 407, worst case 1664). Both exceeded the NNV in the general population (1840; best case 1196, worst case 3066). NNVs for surgical patients remained favourable at a range of SARS-CoV-2 incidence rates in sensitivity analysis modelling. Globally, prioritizing preoperative vaccination of patients needing elective surgery ahead of the general population could prevent an additional 58 687 (best case 115 007, worst case 20 177) COVID-19-related deaths in 1 year. Conclusion: As global roll out of SARS-CoV-2 vaccination proceeds, patients needing elective surgery should be prioritized ahead of the general population