Primers used for PCR-amplification of the Tmo-4C4 nuclear locus and the cytochrome <i>b</i> and cytochrome <i>c</i> oxidase I genes.

<p>Primers used for PCR-amplification of the Tmo-4C4 nuclear locus and the cytochrome <i>b</i> and cytochrome <i>c</i> oxidase I genes.</p

Maximum likelihood tree reconstructed from the <i>Apistogramma</i> concatenated haplotype dataset of the mitochondrial cytochrome <i>b</i> and cytochrome <i>c</i> oxydase I genes and the Tmo-4C4 nuclear locus.

<p>Haplotypes are detailed in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182618#pone.0182618.s006" target="_blank">S2 Table</a>. Numbers at nodes are for bootstrap percentages (≥ 50%) and posterior probabilities (≥ 0.85). Black circles indicate nodes with BP = 100% and PP = 1.00, while grey circles are for nodes with a weak support (BP < 50% and PP < 0.85). Nodes with “-”are weakly supported in maximum likelihood approach or Bayesian inference. Branches with “*” indicate short internal branches not significantly different from zero.</p