THE ANTI-INFLAMMATORY EFFECT OF GARDI PATCH IN CARRAGEENAN-INDUCED PAW EDEMA OF RATS

Gardi -5 has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The drug has been studied in chemical-pharmacological research. We have a new natural patch from the prepared Gardi-5. The present work was undertaken to evaluate the traditional drug Gardi patch for its anti-inflammatory activity.The method of Miho Sekiquichi was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Gardi patch and Ketoprofen patch one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the sub plantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 30, 60, 120, 180, 240, 300 mins using Plethysmometer (UGO Basile, Italy). The right hind paw was injected with 0.1 ml of vehicle.The Gardi patch significantly (p=0.000) inhibited carrageenan induced rat paw edema as compared to control group. In assay data, the TNF-α, PGE2 secretion in serum were highly elevated by carrageenan induction but administration of Gardi patch significantly reduced serum secretion of inflammatory mediators as compared to vehicle group. Gardi patch has an anti-inflammatory properties

SOME RESULT OF QUALITY PARAMETERS OF “GARDI” TRANSDERMAL PATCH

The aim of this study was to conduct standardization study and determine quality parameters of “Gardi” transdermal patch. We used “Gardi” transdermal patch consists of extract of radix Aconitum Kusnezoffii, extract of Terminalia chebula, extract of Saussurea lappa L., extract of Acorus calamus L. and Musk of Musk deer. We used some biologically active compounds of the transdermal patch were revealed by TLC. And main biologically active compounds content of the transdermal patch were determined by spectrophotometric methods. The moisture, average weight and parameters of microbiology were determined by Mongolian National First Pharmacopoeia methods. The transdermal patch was light brown colour, unusual smell and rectangular shape. We determined aconitine, costunolide and gallic acid in “Gardi” transdermal patch by TLC. The retention time of aconitine was 63.04±0.2 minutes and amount was 0.169±0.009% by HPLC.The polyphenolic compounds by spectrophotometer method using Folin-Chiocalto reagent as 0.567±0.043%. Quality and safety parameters of “Gardi” transdermal patch determined as: moisture 27.28±0.65 %, average weight 3.969±0.196 g, thickness 0.83±0.2 mm, total bacteria 1х103, mould, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus and Enterobacter were not detected in “Gardi” transdermal patch

In Memoriam, Academician Prof. Dr. Osor Shagdarsuren (1929-2010)

Academician, Professor Osor Shagdarsuren passed away due to apoplexy on Tuesday, February 2, 2010, at the age of 81. He was one of the most respected Mongolian ornithologists, biologists, and educators. The Mongolian scientific community has lost one of its greatest members, the premier Mongolian ornithologist