Isolation and Characterization of Endophytic Bacteria in Ciplukan Plant (Physalis angulata)

Biodiversity of endophytic bacteria is a potential biological resource to be applied in various fields, especially in the medical field. Endophytic bacteria are bacteria that live in the host plant tissues which belongs to a symbiosis of mutualism. Ciplukan plant (Physalis angulata) is a herbal plant that is useful as antihyperglycemic, antimicrobe, immunostimulant, anti-inflammatory, antioxidant, and cytotoxic. Active compounds obtained from plants need a more complicated time and process compared to extracting compounds from bacteria. The study aims to obtain isolates and characterization of endophytic bacteria in Ciplukan plant (Physalis angulata). The method that is used is the isolation of microbe by Nutrient Agar (NA), and morphological characterization with a gram staining technique. The result of isolation of endophytic bacteria in Ciplukan plant (Physalis angulata) obtains the data which the highest mean is in the rhizosphere part that is 13.13 x 106. Isolation of a single colony of endophytic bacteria obtains 20 bacterial isolates which are 12 bacterial isolates of Gram-positive and 8 bacterial isolates of Gram-negative. The result of isolation of a single colony obtains 1 bacterial isolate of endophytic root (AC2) that has characteristic as Actinobacteria which has a form of spherical spore of colony and Gram-positive. Actinobacteria colonies grow by attached to the surface of the media and can produce spores such as powder. Metabolite compounds produced by Actinobacteria have the role of inhibiting the growth of bacteria and fungi. Furthermore, the metabolites produced by Actinomycetes are widely developed as medicinal materials that can cope with various diseases

INVENTORY OF MACROFUNGI DIVERSITY ON THE CANOPY TRACK AND CIPADARANTEN 1 TRACK IN THE PPKA BODOGOL AREA, SUKABUMI, WEST JAVA

The inventory of macrofungi diversity on two routes, namely the Kanopi route and the Cipadaranten 1 route at the Bodogol Nature Conservation and Education Center (PPKA), aims to determine the diversity of macrofungi species that live in the area. The research method used is direct observation in the Canopy and Cipadaranten 1 route areas. With the condition of the trees on both routes which are still quite dense and the soil area is still damp due to the cover of the trees, there is great potential for the growth of various types of macrofungi. The results obtained during research on both routes and identification produced 24 species of macrofungi Microporus sp. 1, Microporus sp. 2, Microporus sp. 3, Trametes sp.1, Lenzites sp, Ganoderma sp., Ganoderma boninense, Trametes sp.2, Microporus xanthopus, Hexagonia sp, Trametes parvispora, Microporus sp. 4, Hygrocybe aurantiosplendens, Hygrocybe sp., Marasmius sp. 1, Collybia aurea, Crepidotus sp., Volvariella sp., Marasmius sp. 2, Lycoperdon sp., Marasmiellus sp. , Coprinus sp. , Stereum sp., Auricularia sp. The results obtained prove that the diversity of macrofungi types on the Kanopi and Cipadaranten 1 routes in PPKA Bodogol, West Java is very diverse and varied

Foot Print of Macro Fungi in The Coastal Forest of Bama, Baluran National Park, East Java

Baluran National Park, West Java, as one of the conservation sites in Indonesia, has the attraction of the varied types of ecosystems, including fungi. This study aimed to analyze the diversity of fungi in Bama Coastal Forest, Baluran National Park. The method was explorative with plot purposive sampling technique. Parameters in this study include abundance, dominance, and diversity of fungi enriched with physical parameters of humidity and temperature. The fungi were documented and macroscopically observed. Data were analyzed using the abundance index, dominance index, and diversity index. This research identified 18 types of macrofungi in Bama Coastal forest, Baluran National Park East Java including Ganoderma, sp, Hexagonia tenuis, Trametes hirsute, Phellinus sp.1 and sp.2, Ganoderma applanatum, Phellinus igniarius, Pycnoporus cinnabarinus, Daedalea quercina, Tyromyces chioneus, Microporus xanthopus, Calvatia sp., Irpex lacteus, Trichaptum sp., Lentinus sp. Poria corticola, Tyromyces sp., and Lichemomphalia sp. One fungi species (Ganoderma sp.) has the highest abundance index (27.62). The Medium abundance index was found in three species (Hexagonia tenuis, Tyromyces  chioneus, Tyromyces sp.) while the rest fungi were in low abundance index. Dominance index of all fungi species identified in Bama was in low category (D<0.5) with medium diversity (2.30). It can be concluded that the diversity of microfungi in the coastal forest of Bama, Baluran National park was a distribution medium of individual of each species and medium community stability. Environmental conditions in the Bama Coast Forest with 93% humidity and an average air temperature of 26-27oC could support for the fungi to grow quite well

The Potency of Purple Sweet Potato (Ipomoea batatas) Leaf Extract as Biofungicide for Controlling Fusarium Rot on Chili

Fusarium sp. has been recognized as a pathogenic agent causing fruit rot in chili. The disease significantly decreases the annual chili production rate. One of the plants potentially utilized as biofungicide to control Fusarium fruit rot disease in chili is purple sweet potato (Ipomoea batatas L.). Purple sweet po-tato contains secondary metabolites, mainly the active compounds in the form of flavonoids, which has been previously revealed to perform antifungal activity. This study aimed to examine the potential of purple sweet potato (Ipomoea batatas L.) leaf extract as biofungicide to inhibit the growth of chili rot disease caused by Fusarium sp. within vitro and in vivo assay. This work was conducted by testing the ethanol-extracted purple sweet potato leaves extracts against Fusarium sp. with various concentrations, i.e., 5%, 10%, 20%, and 40% (w/v) for in vitro assay. Then antifungal test against Fusarium sp. was conducted using preventive and curative test for in vivo assay. Observa-tions were performed for 7 days. The result of in vitro assay showed inhibi-tory ability of the leaves extract against the growth of Fusarium sp. to range between 35.2-76.6% with potential concentration 40% (w/v). Based on the results of in vivo assay, the preventive test using 40% concentration of purple sweet potato leaves extract showed significant different against negative con-trol through Duncan test (α = 0.05). Purple sweet potato leaves extract with 40% concentration can reduce the intensity of Fusarium fruit rot disease and potentially utilized as biofungicide

Proceedings of the 3rd International Conference on Community Engagement and Education for Sustainable Development

This proceeding contains articles on the various ideas of the academic community presented at The 3rd International Conference on Community Engagement and Education for Sustainable Development (ICCEESD 2022) organized by the Universitas Gadjah Mada, Indonesia on 7th-8th December 2022.  ICCEESD is a biannual forum for sharing, benchmarking, and discussing HEI’s activities in developing Education for Sustainable Development towards community engagement. Education for Sustainability as a teaching strategy for resolving community challenges through formal, informal, or non-formal education is expected to benefit from various community service best practices by academics, researchers, and students. The 3rd ICCEESD has “Strengthening Education for Sustainability Towards Better Community Engagement” as its theme this year. It is expected that the 3rd ICCEESD will provide a forum for the presenters and participants to exchange best practices, policies, and conceptual implementation of Education for Sustainability towards better community engagement and explore ideas to address community needs.  Conference Title: 3rd International Conference on Community Engagement and Education for Sustainable DevelopmentConference Theme: Strengthening Education for Sustainability Towards Better Community EngagementConference Acronyms: ICCEESD 2022Conference Date: 7th-8th December 2022Conference Location: Grand Rohan Jogja Yogyakarta, IndonesiaConference Organizer: Universitas Gadjah Mada, Indonesi