INFLUENCE OF GRAPE SEED EXTRACT SOLUTION ON MICROLEAKAGE BETWEEN COMPOSITE RESIN AND DENTIN

Objectives: The purpose of this study is to evaluate the influence of grape seed extract (GSE) solution as dentin biomodification on microleakagebetween composite resin and dentin.Methods: A total of 52 dentin specimens were divided into two groups. Group 1 involved the application of GSE solution for 30 seconds after etching.Group 2 served as the control group.Results: The GSE solution group significantly increases microleakage between the composite resin and dentin compared to the control group.Conclusions: The application of GSE solution after etching can increase microleakage between composite resin and dentin

Penutupan Diastema dengan Menggunakan Komposit Nanofiller

The purpose of this work is to report a clinical care of diastema closure in case of multiple diastema using composite nanofiller material, as an alternative from orthodontic treatment and also porcelain crown restoration. This method proved to be conservative, practical, and provide high estethic value.DOI: 10.14693/jdi.v15i3.3

EFFECTIVITY OF TWO BLEACHING AGENT OF 10% CARBAMIDE PEROXIDE WITH AND WITHOUT POTASSIUM NITRATE-FLUORIDE (CLINICAL STUDY)

Tooth bleaching has become a popular treatment for esthetic improvement in dentistry. There are several 10% carbamide peroxide bleaching agents that are available in Indonesia which contained potassium nitrate-fluoride or without potassium nitrate-fluoride. However, there was no clinical report about these products in Indonesia. This study was conducted to compare the effectiveness of two bleaching and sensitivity of tooth and gingiva. Sixty-four participants were divided into 2 groups. The first group was treated with bleaching agent that contained potassium nitrate-fluoride (Opalescence PF, Ultradent). Bleaching treatment was done for 6-8 hours per night over a 2 week-period. Evaluations were performed at the baseline and at 3, 7, 14 day afterwards. Color change was measured using a value-ordered Vita classic shade guide; tooth and gingival sensitivity were examine using Electric Pulp Tester, Gingival Index and patient log. The results showed that there were no statistical differences in degree of color change between the two products. The mean color change after 2 weeks was 7-8 tabs lighter than baseline. Also there was no statistical difference in tooth and gingival sensitivity between the products. It can be concluded that 10% carbamide peroxide containing potassium nitrate-fluoride has the same effectiveness compared to other agent without potassium nitrate-fluoride for tooth color change and tooth and gingival sensitivity

COMPARISON OF DENTINAL TUBULAR PENETRATION OF THREE BIOCERAMIC SEALERS

Objective: The main objective of endodontic treatment is to remove microorganisms from the root canal space and prevents reinfection. Deep penetration of the dentinal tubule is advantageous because it increases the contact area between the root canal filling material and dentin, thereby increasing the sealing quality of the entire root canal system. Bioceramic sealers are biocompatible, nontoxic, non-shrinking, hydrophilic, and stable, do not expand during setting, and can form hydroxyapatite, which then forms chemical bonds with dentin to compare the abilities of three types of bioceramic-based sealers to penetrate the dentinal tubules.Methods: Obturation used three types of bioceramic sealers. Group 1 (calcium phosphate silicate), Group 2 (a mixture of tricalcium silicate and resin), and Group 3 (pure tricalcium silicate) were observed using a scanning electron microscope and measurement of the penetration distance with ImageJ.Results: The bioceramic sealers had statistically significant differences in penetration distance into dentinal tubules (p’s<0.001). The mean penetration value of Group 1 (calcium phosphate silicate) was 115.99 μm, Group 2 (a mixture of tricalcium silicate and resin) was 209.28 μm, and Group 3 (pure tricalcium silicate) was 84.07 μm.Conclusion: Although all three bioceramic sealers penetrated the dentinal tubules, they exhibited differences in their penetration capabilities. Group 2 had the deepest penetration, likely due to the resin content

EFFECTS OF COLD LATERAL VERSUS WARM VERTICAL COMPACTION OBTURATION ON THE PUSH-OUT BOND STRENGTH OF BIOROOT™, A CALCIUM SILICATE-BASED SEALER

Objective: BioRoot™ , which contains pure calcium silicate, is used in cold lateral compaction. However, hydroxyl ions are still released when BioRoot™is used in warm vertical compaction. This study compared the effects of cold and warm vertical compaction obturation on the push-out bond strengthof BioRoot™.Methods: Specimens from 16 root canals instrumented with ProTaper Next X5 50/06 were divided into two groups (n=16 specimens per group).Group 1 was obturated using cold lateral compaction, whereas Group 2 was obturated using warm vertical compaction. All samples were incubatedfor 48 h (37°C, 100% humidity) and embedded into an acrylic block. Starting at 7 mm from the apex, two 2-mm-thick slices of each sample were cut.Dislodgement resistance was measured using a universal testing machine, and the push-out bond strength was calculated.Results: There was a significant difference in the push-out bond strength value between cold (4.5–41.1 MPa) and warm (4.12–24.25 MPa) compactionobturation (p<0.05).Conclusion: Cold lateral compaction provides better adhesion capability than warm vertical compaction in root canal obturation

COMPARISON OF THREE BIOCERAMIC SEALERS IN TERMS OF DENTINAL SEALING ABILITY IN THE ROOT CANAL

Objective: The main objective of endodontic treatment is to prevent further infection or reinfection by eliminating microorganisms within theroot canal system. Proper endodontic treatment could prevent apical and coronal penetration of fluids and microorganisms. Endodontic sealer isvital components of root canal obturation to establish a fluid-tight seal. Bioceramic-based root canal sealers are considered to be an advantageoustechnology in endodontics and have been found to be both biocompatible and comparable to other commercial sealers. The aim of this study was tocompare the adhesion of three bioceramic sealers within the root canal system.Methods: Endodontically treated teeth were obturated using three types of bioceramic sealers and then divided into three groups. Specimens werethen observed using a scanning electron microscope, and the attachment distance was measured using ImageJ.Results: The three groups exhibited were statistically significant differences (p<0.05) in dentinal sealing ability. Calcium phosphate silicate-basedsealer showed the highest sealing ability, followed by pure tricalcium silicate-based bioceramic sealers and then tricalcium silicate and resin-basedbioceramic sealers.Conclusion: The sealing ability of calcium phosphate silicate-based sealer is superior to that of both pure tricalcium silicate-based and tricalciumsilicate- and resin-based sealer

THE INFLAMMATORY REACTION OF PULP TISSUE WITH CALCIUM HYDROXIDE, MTA AND PORTLAND CEMENT (In Vivo)

Background : Perforation in normal pulp could be happened anytime. In sterile condition, direct pulp capping was the right therapy to maintain the vitality and pulp function. Direct pulp capping agent must contact with the pulp tissue. Generally, body had an immunologic respond with foreign body that contact with tissue. The respond could be inflammatory reaction. The used direct pulp capping agents were calcium hydroxide, and Mineral Trioxide Aggregate (MTA) which known as better agent but relatively expensive and difficult to obtain. So that, the alternative of MTA, Portland cement which was the same essential of MTA, was being developed. The aim of this study, to analized inflammatory reaction of the pulp tissue with direct pulpcapping agents calcium hydroxide, MTA, and Portland cement. Methods: Free caries M. Nemestrina’s posterior teeth were prepared to form pin point perforations on buccal surface of the crowns. The teeth were applied with one of the three capping agents. Teeth were filled and extracted. The extracted teeth were proceed into histopatological preparation slides to evaluate the inflammatic reaction. Results: The result showed no statistically significant differences of pulp inflammatory reaction between calcium hydroxide, MTA and Portland cement in 7, 14, 42, and 90 days. Conclusion: The inflammatory reaction of pulp tissue of the three pulp capping agent (calcium hydroxide, MTA, and PC) were insignificant different

COMPARISON OF HUMAN PLATELET LYSATE AND FETAL BOVINE SERUM IN CULTURE MEDIA FOR HUMAN DENTAL PULP STEM CELL PROLIFERATION

Objective: Ex vivo and in vitro cell cultures require a basal medium with added supplements containing growth factors, proteins, and enzymes tosupport attachment, growth, and proliferation. Fetal bovine serum (FBS) is used to supplement cell culture media. However, human platelet lysate(hPL) represents an attractive alternative as it is nonxenogeneic.Methods: Human third molars were collected from six healthy donors (19–35 years old) with no history of regular alcohol consumption or smoking.Human dental pulp stem cells (hDPSCs) at the second passage were divided into two culture media groups, 10% FBS and 5% hPL, as well as a controlgroup after 24 h of serum starvation. A flow cytometry analysis was conducted to measure CD90, CD105, CD73, CD34, CD45, and Human LeukocyteAntigen-DR isotype (HLA-DR). Cellular proliferation was evaluated on days 1, 3, and 5.Results: The flow cytometry analysis revealed that the majority of the cells expressed positive mesenchymal stem cell surface markers, includingCD73 (98.5%), CD90 (98.3%), and CD105 (71.0%), and lacked CD34, CD45, and HLA-DR. There were significant differences among the 5% hPL, 10%FBS, and control groups on days 1, 3, and 5.Conclusion: For a nonxenogeneic culture, 5% hPL can be used as an alternative in culture media for hDPSC proliferation

EFFECTIVITY OF TWO BLEACHING AGENT OF 10% CARBAMIDE PEROXIDE WITH AND WITHOUT POTASSIUM NITRATE-FLUORIDE (CLINICAL STUDY)

Tooth bleaching has become a popular treatment for esthetic improvement in dentistry. There are several 10% carbamide peroxide bleaching agents that are available in Indonesia which contained potassium nitrate-fluoride or without potassium nitrate-fluoride. However, there was no clinical report about these products in Indonesia. This study was conducted to compare the effectiveness of two bleaching and sensitivity of tooth and gingiva. Sixty-four participants were divided into 2 groups. The first group was treated with bleaching agent that contained potassium nitrate-fluoride (Opalescence PF, Ultradent). Bleaching treatment was done for 6-8 hours per night over a 2 week-period. Evaluations were performed at the baseline and at 3, 7, 14 day afterwards. Color change was measured using a value-ordered Vita classic shade guide; tooth and gingival sensitivity were examine using Electric Pulp Tester, Gingival Index and patient log. The results showed that there were no statistical differences in degree of color change between the two products. The mean color change after 2 weeks was 7-8 tabs lighter than baseline. Also there was no statistical difference in tooth and gingival sensitivity between the products. It can be concluded that 10% carbamide peroxide containing potassium nitrate-fluoride has the same effectiveness compared to other agent without potassium nitrate-fluoride for tooth color change and tooth and gingival sensitivity