Comparison of analgesic efficacy of perineural dexamethasone in rectus sheath block and intravenous dexamethasone with levobupivacaine in bilateral rectus sheath block for patients undergoing midline abdominal surgery: a randomized controlled trial

Abstract Background Dexamethasone is used in regional analgesia to prolong the duration of analgesia and anaesthesia. The perineural and intravenous (IV) dexamethasone in fascial plane block have conflicting results. The study aimed to compare analgesic efficacy of perineurally and intravenously dexamethasone in rectus sheath block (RSB). This prospective, double-blinded, randomized control study was conducted in 105 patients, equally divided in three groups. All the patients received ultrasound-guided RSB block with 20 ml of 0.25% levobupivacaine on each side. Additionally, in group A, 1-ml normal saline (NS) on each side in RSB block and 2-ml NS IV; in group B, 4-mg dexamethasone on each side in RSB block and 2-ml NS IV; and in group C, 1-ml NS on each side in RSB block and 8-mg dexamethasone IV. The primary objective of the study was the duration of analgesia, and secondary objectives were total morphine consumption in the first 24 h, numeric rating scale (NRS) scores at rest and on cough, complications and patient’s satisfaction score. Results The duration of analgesia was maximum in group B (935.91 ± 121.82 min) and then in group C (730.31 ± 129.64 min) and group A (418.34 ± 29.22 min) (P < 0.0001). The morphine consumption and mean NRS score (at rest and cough) were lowest in group B and then group C and group A (P < 0.0001). Conclusions The perineural dexamethasone as an adjuvant to levobupivacaine prolongs the duration of analgesia with decreased analgesic requirements compared to intravenous dexamethasone in bilateral RSB in patients undergoing midline laparotomy surgery

Genomic and Functional Characterization of a Novel Burkholderia sp. Strain AU4i from Pea Rhizosphere Conferring Plant Growth Promoting Activities

Members of Burkholderia genus are gaining importance for their application in crop improvement by acting as plant growth-promoting rhizobacteria (PGPR), however, our knowledge about mechanisms of their plant growth promotion is limited. Herein, we aimed to isolate Burkholderia sp. from pea rhizosphere that confers plant growth promoting traits both in vitro and in vivo using pea seedlings as model. We have isolated a novel Burkholderia strain AU4i (B-AU4i) from pea rhizosphere that strongly promotes root and shoot growth in plantae. B-AU4i confers phosphate solubilization, indole-3-acetic acid production, N2 fixation, ammonia production, siderophore production, HCN production, and inhibits growth of pathogenic fungi both in vitro and in vivo experiments where we employed pea-seedlings as model system. Sequencing of B-AU4i genome using the Illumina-HiSeq 1000 technology reveals that it contains genes for the above-mentioned biofertilizer and biocontrol activities. The findings demonstrate the potential use of B-AU4i as plant growth promoter, which could be due to the presence of relevant genes in its genome. The current study improves our knowledge regarding the genes present in Burkholderia spp. that are involved in its plant growth promotion activities. B-AU4i strain can be used for improving agriculture productivity owing to its strong biofertilizer and biocontrol activity.Fil: Usha, Devi. Shoolini University; IndiaFil: Khatri, Indu. Council of Scientific & Industrial Research. Institute of Microbial Technology; IndiaFil: Lalit, Kumar. Shoolini University; IndiaFil: Devender, Singh. Shoolini University; IndiaFil: Aditi, Gupta. Shoolini University; IndiaFil: Navinder, Kumar. Council of Scientific & Industrial Research. Institute of Microbial Technology; IndiaFil: Gárriz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Subramanian, Srikrishna. Council of Scientific & Industrial Research. Institute of Microbial Technology; IndiaFil: Sharma, Deepak. Council of Scientific & Industrial Research. Institute of Microbial Technology; IndiaFil: Adesh, Saini K.. Shoolini University; Indi