LAK cells (A) or different subpopulations (B) were stained for granulysin and CD surface markers

<p><b>Copyright information:</b></p><p>Taken from "Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes"</p><p>http://www.biomedcentral.com/1471-2172/8/9</p><p>BMC Immunology 2007;8():9-9.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1914365.</p><p></p> Cells were analysed using confocal laser scanning microscope by counting 500 cells pro sample. Graph (C) represents phenotype analysis of LAK cells. Four different donors were analysed and each symbol represents one donor. Bars indicate mean values. (* p < 0.05; * * p < 0.005; * * * p < 0.0005

Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes-4

<p><b>Copyright information:</b></p><p>Taken from "Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes"</p><p>http://www.biomedcentral.com/1471-2172/8/9</p><p>BMC Immunology 2007;8():9-9.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1914365.</p><p></p>r 10 μm

Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes-0

<p><b>Copyright information:</b></p><p>Taken from "Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes"</p><p>http://www.biomedcentral.com/1471-2172/8/9</p><p>BMC Immunology 2007;8():9-9.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1914365.</p><p></p>d in LAK or antigen specific T-cells. Black boxes indicate coding and gray boxes noncoding exon parts. Exons are marked with roman numbers. Dotted lines correspond to intron regions. Arabic numbers 1 to 7 stay for primers numbered in the same way as in Table 1

Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes-8

<p><b>Copyright information:</b></p><p>Taken from "Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes"</p><p>http://www.biomedcentral.com/1471-2172/8/9</p><p>BMC Immunology 2007;8():9-9.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1914365.</p><p></p>les were taken for RT-PCR analysis. Representative gel image shows transcripts present in LAK cells over the stimulation period (A). The same transcripts were found in different subpopulations (not shown). Graph (B) represents results of semiquantitative analysis of the transcripts normalized to actin (gel image, lines A) in LAK cells from three different donors. Each symbol represents one donor and bars correspond to the mean value. Graph (C) shows the same analysis in different subpopulations where one representative donor is shown

Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes-2

<p><b>Copyright information:</b></p><p>Taken from "Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes"</p><p>http://www.biomedcentral.com/1471-2172/8/9</p><p>BMC Immunology 2007;8():9-9.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1914365.</p><p></p>ds. Samples were collected before stimulation (day 0), before restimulation (day 6) and 6 days after restimulation (day 12). PCR was performed and analysed by gel electrophoresis. Values are results from semiquantitative analysis of transcripts normalised to actin from the same cDNA. Each symbol represents one donor

Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes-5

<p><b>Copyright information:</b></p><p>Taken from "Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes"</p><p>http://www.biomedcentral.com/1471-2172/8/9</p><p>BMC Immunology 2007;8():9-9.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1914365.</p><p></p>. Cells were analysed with a confocal laser scanning microscope counting 300 cells. Graph (A) shows granulysin expression in antigen specific T-cells as a whole population; (B) represent phenotype of specific T cells; (C) phenotype of L. specific T cells graph (D) represent granulysin expression in different subsets in specific T cells and graph (E) in specific T cells. In (B), (C), (D), and (E) every donor is indicated with one symbol. Bars are mean values. (*** p < 0.0005

Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes-7

<p><b>Copyright information:</b></p><p>Taken from "Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes"</p><p>http://www.biomedcentral.com/1471-2172/8/9</p><p>BMC Immunology 2007;8():9-9.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1914365.</p><p></p>d in LAK or antigen specific T-cells. Black boxes indicate coding and gray boxes noncoding exon parts. Exons are marked with roman numbers. Dotted lines correspond to intron regions. Arabic numbers 1 to 7 stay for primers numbered in the same way as in Table 1

Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes-6

<p><b>Copyright information:</b></p><p>Taken from "Expression, processing and transcriptional regulation of granulysin in short-term activated human lymphocytes"</p><p>http://www.biomedcentral.com/1471-2172/8/9</p><p>BMC Immunology 2007;8():9-9.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1914365.</p><p></p>f cells and separation of proteins by SDS-PAGE. Proteins were blotted and detected using anti-granulysin Ab (1:1000). The blot was stripped and probed for the β-actin

HASMC were infected with Cpn-K6 128 IFU/cell (A, B, C, D), Cpn-VR1310 32 IFU/cell (E, F) for 72 h or were inoculated with mock lysate (G) or treated with staurosporin 1 μM for 14 h (H)

<p><b>Copyright information:</b></p><p>Taken from "induces aponecrosis in human aortic smooth muscle cells"</p><p>BMC Microbiology 2005;5():2-2.</p><p>Published online 21 Jan 2005</p><p>PMCID:PMC547904.</p><p>Copyright © 2005 Dumrese et al; licensee BioMed Central Ltd.</p> The cells were subsequently stained for TUNEL (green), NHS-biotin (brown), anti-Cpn-MOMP (red) and DNA (blue). A: HASMC bearing a Cpn-K6 inclusion. DAPI stained chromatin structure is normal and TUNEL as well as NHS-biotin labeling are negative. B: Cpn-K6 infected HASMC with multiple chlamydial spots (arrow heads) and normal nuclear structure. C, E: Cpn-K6 (C) or Cpn-VR1310 (E) infected HASMC with spot like infection (arrow heads). The condensed nucleus is TUNEL positive, the cytoplasm is diffusely labeled with NHS-biotin. D, F: HASMC with Cpn-K6 (D) or Cpn-VR1310 (F) in aggregated (arrow) spots and single spots (arrow heads). Note positive TUNEL labeling of the shrunken nucleus and NHS-biotin staining of the cytoplasm. G: Mock inoculated HASMC. Note the fine granular chromatin structure and lack of TUNEL- and cytoplasmic NHS-biotin staining. H: HASMC incubated with staurosporin. The nucleus exhibits a distinct nuclear fragmentation (DAPI staining) but no TUNEL labeling. The cytoplasm is NHS-biotin negative indicating an intact cell membrane. I: Quantification of TUNEL positive nuclei depending on the chlamydial infectious dose 72 h post infection reveals a dose dependent increase of TUNEL positive cells. Cells in 5 random fields with an area of 62'500 μmwere counted and the percentage of TUNEL positive cells was calculated. -▲-: Cpn-K6; -■-: Cpn-VR131

Cells infected with Cpn-K6 in doses between 8 – 386 IFU/cell or with Cpn-VR1310 in doses between 1 – 32 IFU/cell were stained with annexin-V / propidium iodide and analyzed by flow cytometry 48 h post infection

<p><b>Copyright information:</b></p><p>Taken from "induces aponecrosis in human aortic smooth muscle cells"</p><p>BMC Microbiology 2005;5():2-2.</p><p>Published online 21 Jan 2005</p><p>PMCID:PMC547904.</p><p>Copyright © 2005 Dumrese et al; licensee BioMed Central Ltd.</p> Mock inoculated cells are shown as controls. In figure A and B pooled data out of three independent experiments are displayed. A: The amount of single annexin-V positive cells increased dependent on the chlamydial infectious dose (-◆ -: Cpn-K6; -●-: Cpn-VR1310) but not in mock treated (-○-) cells. (*: significant compared to mock treated cells with p < 0.001) B: the number of single annexin-V positive cells increased over the time when cells were infected with Cpn-K6 128 IFU/cell (-●-) but not if they were mock treated (-○-) (significant compared to mock treated cells with p < 0.001)(#)). C: Dot plots of one representative experiment are depicted to illustrate the amount of annexin V / propidium iodide double labeled cells. Upper left panel: HASMC treated with staurosporin (apoptosis). Upper right panel: HASMC treated with Na-azide (necrosis). Middle left panel: HASMC infected with Cpn-VR1310 at 32 IFU / cell 48 h post infection. Middle right panel: HASMC infected with Cpn-K6 at 128 IFU / cell 48 h post infection. Lower left panel: mock treated HASMC