Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines

<p>Abstract</p> <p>Background</p> <p>The immunological response to solid tumours is insufficient. Therefore, tumour specific antigens have been explored to facilitate the activation of the immune system. The cancer/testis antigen class of MAGE-A antigens is a possible target for vaccination. Their differential expression profiles also modulate the course of the cancer disease and its response to antineoplastic drugs.</p> <p>Methods</p> <p>The expression profiles of MAGE-A2, -A3, -A4, -A6 and -A10 in five own oral squamous cell carcinoma cell lines were characterised by rt-PCR, qrt-PCR and immunocytochemistry with a global MAGE-A antibody (57B) and compared with those of an adult keratinocyte cell line (NHEK).</p> <p>Results</p> <p>All tumour cell lines expressed MAGE-A antigens. The antigens were expressed in groups with different preferences. The predominant antigens expressed were MAGE-A2, -A3 and -A6. MAGE-A10 was not expressed in the cell lines tested. The MAGE-A gene products detected in the adult keratinocyte cell line NHEK were used as a reference.</p> <p>Conclusion</p> <p>MAGE-A antigens are expressed in oral squamous cell carcinomas. The expression profiles measured facilitate distinct examinations in forthcoming studies on responses to antineoplastic drugs or radiation therapy. MAGE-A antigens are still an interesting aim for immunotherapy.</p

Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma

<p>Abstract</p> <p>Background</p> <p>acantholytic squamous cell carcinomas (ASCC) and intraoral angiosarcoma share similar histopathological features. Aim of this study was to find marker for a clear distinction.</p> <p>Methods</p> <p>Four oral acantholytic squamous cell carcinomas and one intraoral angiosarcoma are used to compare the eruptive intraoral growth-pattern, age-peak, unfavourable prognosis and slit-like intratumorous spaces in common histological staining as identical clinical and histopathological features. Immunohistochemical staining for pancytokeratin, cytokeratin, collagen type IV, γ2-chain of laminin-5, endothelial differentiation marker CD31 and CD34, F VIII-associated antigen, Ki 67-antigen, β-catenin, E-cadherin, α-smooth-muscle-actin and Fli-1 were done.</p> <p>Results</p> <p>Cytokeratin-immunoreactive cells can be identified in both lesions. The large vascularization of ASCC complicates the interpretation of vascular differential markers being characteristic for angiosarcoma. Loss of cell-cell-adhesion, monitored by loss of E-cadherin and β-catenin membrane-staining, are indetified as reasons for massive expression of invasion-factor ln-5 in ASCC and considered responsible for unfavourable prognosis of ASCC. Expression of Fli-1 in angiosarcoma and cellular immunoreaction for ln-5 in ASCC are worked out as distinguishing features of both entities.</p> <p>Conclusion</p> <p>Fli-1 in angiosarcoma and ln-5 in ASCC are distinguishing features.</p

Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma-3

, ×150).<p><b>Copyright information:</b></p><p>Taken from "Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma"</p><p>http://www.head-face-med.com/content/4/1/17</p><p>Head & Face Medicine 2008;4():17-17.</p><p>Published online 31 Jul 2008</p><p>PMCID:PMC2515303.</p><p></p

Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma-6

<p><b>Copyright information:</b></p><p>Taken from "Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma"</p><p>http://www.head-face-med.com/content/4/1/17</p><p>Head & Face Medicine 2008;4():17-17.</p><p>Published online 31 Jul 2008</p><p>PMCID:PMC2515303.</p><p></p

Oral angiosarcoma: immunohistochemical demonstration of factor VIII-related antigen in a subset of the tumour cells lining the vascular spaces (×150)

<p><b>Copyright information:</b></p><p>Taken from "Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma"</p><p>http://www.head-face-med.com/content/4/1/17</p><p>Head & Face Medicine 2008;4():17-17.</p><p>Published online 31 Jul 2008</p><p>PMCID:PMC2515303.</p><p></p

Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma-5

<p><b>Copyright information:</b></p><p>Taken from "Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma"</p><p>http://www.head-face-med.com/content/4/1/17</p><p>Head & Face Medicine 2008;4():17-17.</p><p>Published online 31 Jul 2008</p><p>PMCID:PMC2515303.</p><p></p

Oral acantholytic squamous cell carcinoma: venular/glandular-like pattern (H&E, ×150)

<p><b>Copyright information:</b></p><p>Taken from "Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma"</p><p>http://www.head-face-med.com/content/4/1/17</p><p>Head & Face Medicine 2008;4():17-17.</p><p>Published online 31 Jul 2008</p><p>PMCID:PMC2515303.</p><p></p

Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma-0

<p><b>Copyright information:</b></p><p>Taken from "Oral acantholytic squamous cell carcinoma shares clinical and histological features with angiosarcoma"</p><p>http://www.head-face-med.com/content/4/1/17</p><p>Head & Face Medicine 2008;4():17-17.</p><p>Published online 31 Jul 2008</p><p>PMCID:PMC2515303.</p><p></p