Controlling skyrmion bubble confinement by dipolar interactions

Large skyrmion bubbles in confined geometries of various sizes and shapes are investigated, typically in the range of several micrometers. Two fundamentally different cases are studied to address the role of dipole-dipole interactions: (I) when there is no magnetic material present outside the small geometries and (II) when the geometries are embedded in films with a uniform magnetization. It is found that the preferential position of the skyrmion bubbles can be controlled by the geometrical shape, which turns out to be a stronger influence than local variations in material parameters. In addition, independent switching of the direction of the magnetization outside the small geometries can be used to further manipulate these preferential positions, in particular with respect to the edges. We show by numerical calculations that the observed interactions between the skyrmion bubbles and structure edge including the overall positioning of the bubbles are fully controlled by dipole-dipole interactions

Precession-torque-driven domain-wall motion in out-of-plane materials

Domain-wall (DW) motion in magnetic nanostrips is intensively studied, in particular because of the possible applications in data storage. In this work, we will investigate a novel method of DW motion using magnetic field pulses, with the precession torque as the driving mechanism. We use a one dimensional (1D) model to show that it is possible to drive DWs in out-of-plane materials using the precession torque, and we identify the key parameters that influence this motion. Because the DW moves back to its initial position at the end of the field pulse, thereby severely complicating direct detection of the DW motion, depinning experiments are used to indirectly observe the effect of the precession torque. The 1D model is extended to include an energy landscape in order to predict the influence of the precession torque in the depinning experiments. Although preliminary experiments did not yet show an effect of the precession torque, our calculations indicate that depinning experiments can be used to demonstrate this novel method of DW motion in out-of-plane materials, which even allows for coherent motion of multiple domains when the Dzyaloshinskii-Moriya interaction is taken into account

Anomalous direction for skyrmion bubble motion

Magnetic skyrmions are localized topological excitations that behave as particles and can be mobile, with great potential for novel data storage devices. In this work, the current-induced dynamics of large skyrmion bubbles is studied. When skyrmion motion in the direction opposite to the electron flow is observed, this is usually interpreted as a perpendicular spin current generated by the spin Hall effect exerting a torque on the chiral N\'{e}el skyrmion. By designing samples in which the direction of the net generated spin current can be carefully controlled, we surprisingly show that skyrmion motion is always against the electron flow, irrespective of the net vertical spin-current direction. We find that a negative bulk spin-transfer torque is the most plausible explanation for the observed results, which is qualitatively justified by a simple model that captures the essential behaviour. These findings demonstrate that claims about the skyrmion chirality based on their current-induced motion should be taken with great caution

Controlling the canted state in antiferromagnetically coupled magnetic bilayers close to the spin reorientation transition

Canted magnetization is obtained in ultrathin, antiferromagnetically coupled magnetic bilayers with thicknesses around the spin reorientation transition. The canting angle is controlled by both the magnetic layer thickness and interlayer coupling strength, which are tuned independently. Hysteresis loops are obtained, where magnetization components parallel and transverse to the applied field are measured, and analyzed by comparison to micromagnetic simulations. This enables the canting angle to be extracted and the behavior of the individual layers to be distinguished. Two types of canted systems are obtained with either single-layer reversal or complex, coupled two-layer reversal, under moderate external magnetic fields. Controlling the magnetization canting and reversal behavior of ultra-thin layers is relevant for the development of magnetoresistive random-access memory and spin-torque oscillator devices.This research was funded by the FP7 ERC 3SPIN Advanced Grant and by a European Erasmus Mobility program. This work is part of the research programme of the Foundation for Fundamental Research on Matter (FOM), which is part of the Netherlands Organisation for Scientific Research (NWO). A. Fernández-Pacheco acknowledges support from an EPSRC Early Career Fellowship EP/M008517/1, and from the Winton Foundation

SOX17 expression and its down-regulation by promoter methylation in cervical adenocarcinoma in situ and adenocarcinoma

Aims: SOX17 expression has not been studied in glandular lesions of the uterine cervix like adenocarcinoma in situ (AIS) and invasive adenocarcinomas (AdC), whereas SOX17 promoter CpG island methylation has been reported. Therefore, the aim of this study was to relate the topographical distribution of SOX17 expression and SOX17 methylation status to each other, and to SOX2 expression, human papillomavirus (HPV) type, and physical status of the virus. Methods and results: Immunohistochemistry was used in 45 cases to assess expression of SOX17 and SOX2. SOX17 promoter methylation was determined in 25 cases by means of bisulphite conversion and methylation-specific polymerase chain reaction. SOX17 and SOX2 showed a mutually exclusive expression pattern in normal epithelium, with a sharp delineation in the squamocolumnar junction. SOX17 was found in endocervical columnar and reserve cells, whereas SOX2 was exclusively found in squamous epithelium. In both glandular lesions and cases with coexisting glandular and squamous intraepithelial components, a complex combination of SOX17 and SOX2 expression patterns was seen and mutually exclusive expression was lost. Frequently, gain of expression of SOX2 was found and expression of SOX17 was lost. Methylation of the CpG island in the SOX17 promoter was shown to be strongly associated with loss of expression of SOX17 (P = 0.0016). Conclusions: In this study, we show for the first time a direct correlation between the topographical distribution of SOX17 expression and the methylation status of its gene promoter. This explains the heterogeneity of SOX17 expression in the glandular lesions of the cervix. No correlation was found between HPV type and physical status of the virus on the one hand and methylation status on the other

Switches of SOX17 and SOX2 expression in the development of squamous metaplasia and squamous intraepithelial lesions of the uterine cervix

Aims: The dynamics and topographical distribution of SOX17 and SOX2 expression was studied in the transformation zone (TZ) of the uterine cervix. This TZ is a dynamic area where switches from glandular into squamous epithelium can be recognized, new squamocolumnar junctions are formed, and premalignant lesions originate. SOX17 and SOX2 show mutually exclusive expression patterns in the normal uterine cervix, with SOX2 being exclusively found in squamous epithelium, while SOX17 is detected in endocervical columnar cells and reserve cells. Methods and Results: Normal cervices and squamous intraepithelial lesions (SIL) were studied with immunohistochemistry, methylation of SOX17, human papilloma virus (HPV) genotyping, and in situ hybridization. In the TZ squamous metaplasia originating from these reserve cells can still show SOX17 expression, while also remnants of SOX17-positive immature metaplasia can be recognized in the normal squamous epithelium. SOX17 expression is gradually lost during maturation, resulting in the exclusive expression of SOX2 in the majority of (SIL). This loss of SOX17 expression is independent of methylation of the CpG island in its promotor region. HPV can be detected in SOX17-positive immature metaplastic regions in the immediate vicinity of SOX2-positive SIL, suggesting that switches in SOX17 and 2 expression can occur upon HPV infection. Conclusions: This switch in expression, and the strong association between the distribution of reserve cells and squamous areas within the columnar epithelium in the TZ, suggests that reserve cell proliferations, next to basal cells in the squamous epithelium, are potential targets for the formation of squamous lesions upon viral infection

Proliferation and aneusomy predict survival of young patients with astrocytoma grade II

The clinical course of astrocytoma grade II (AII) is highly variable and not reflected by histological characteristics. As one of the best prognostic factors, higher age identifies rapid progressive A II. For patients over 35 years of age, an aggressive treatment is normally propagated. For patients under 35 years, there is no clear guidance for treatment choices, and therefore also the necessity of histopathological diagnosis is often questioned. We studied the additional prognostic value of the proliferation index and the detection of genetic aberrations for patients with A II. The tumour samples were obtained by stereotactic biopsy or tumour resection and divided into two age groups, that is 18–34 years (n=19) and 35 years (n=28). Factors tested included the proliferation (Ki-67) index, and numerical aberrations for chromosomes 1, 7, and 10, as detected by in situ hybridisation (ISH). The results show that age is a prognostic indicator when studied in the total patient group, with patients above 35 years showing a relatively poor prognosis. Increased proliferation index in the presence of aneusomy appears to identify a subgroup of patients with poor prognosis more accurately than predicted by proliferation index alone. We conclude that histologically classified cases of A II comprise a heterogeneous group of tumours with different biological and genetic constitution, which exhibit a highly variable clinical course. Immunostaining for Ki-67 in combination with the detection of aneusomy by ISH allows the identification of a subgroup of patients with rapidly progressive A II. This is an extra argument not to defer stereotactic biopsy in young patients with radiological suspicion of A II