Possible bacterial strains containing <i>dszABC</i> operon.

<p>Possible bacterial strains containing <i>dszABC</i> operon.</p

Screening of <i>dsz</i> genes in different MTCC strains <i>Rhodococcus rhodochrous</i> (3552), <i>Artrobacter sulfureus</i> (3332), <i>Gordonia rubropertincta</i> (289) and <i>Rhodococcus erythropolis</i> (3951) enriched with DBT.

<p>The different lanes are showing the amplified products of different genes <i>dszA</i>, <i>dszB</i>, <i>dszC</i> and <i>dszD</i>.</p

List of organisms comparing their desulfurizing efficiency in percentage removal of sulfur from literature with reference to the present study.

<p>List of organisms comparing their desulfurizing efficiency in percentage removal of sulfur from literature with reference to the present study.</p

4S-Pathway of biodesulfurization of dibenzothiophene (DBT).

<p>Four enzymes (DszA, DzsB, DszC and DszD) are involved in the pathway where the first three steps catalyzed by flavin mononucleotide reduced (FMNH₂)-dependent monooxygenases, those leading to DBT-sulfoxide (DBTO), DBT-dioxide (DBTO₂) and hydroxyphenyl benzenesulfinate (HPBS), respectively. The final desulfurization step to 2-hydroxybiphenyl (2-HBP) is catalyzed by desulfinase.</p

Standardization of PCR product of <i>dsz</i> genes and 16s rRNA with different annealing temperatures.

<p>The PCR products with different annealing temperature were electrophoresed in agaraose gel against 100 bp DNA ladder.</p

Differential desulfurization of dibenzothiophene by newly identified MTCC strains: Influence of Operon Array - Fig 4

<p>HPLC graph showing the retention time of (a) DBT and (b) 2-HBP. (c) The control DBT without microorganism also shows same retention time with respect to the standard DBT without any degradation.</p

Differential desulfurization of dibenzothiophene by newly identified MTCC strains: Influence of Operon Array - Fig 5

<p>Chromatogram showing the DBT desulfurization after 10 days of growth with different MTCC strains (a) <i>Rhodococcus rhodochrous</i> (3552), (b) <i>Artrobacter sulfureus</i> (3332), (c) <i>Gordonia rubropertincta</i> (289) and (d) <i>Rhodococcus erythropolis</i> (3951).</p