Effect of compaction agent concentration on DNA precipitation.

<p>Three sets of human serum samples containing 10, 1, and 0.1 ng/ml of lambda DNA were heat treated and compaction precipitated with various concentrations of spermine and quatroquat.</p

Detection of small DNA in serum after compaction precipitation.

*<p>Unless otherwise noted (‘twice’) all samples went through one round of the compaction precipitation protocol. Heat: 10 min 95°C. SP: spermine (2 mM), QQ: quatroquat (2 mM), ND: Not detectable.</p

ClustalW multiple sequence alignment of alanine racemases from (SP), DadX (PA) and (MT)

<p><b>Copyright information:</b></p><p>Taken from "Purification and preliminary crystallization of alanine racemase from "</p><p>http://www.biomedcentral.com/1471-2180/7/40</p><p>BMC Microbiology 2007;7():40-40.</p><p>Published online 17 May 2007</p><p>PMCID:PMC1885262.</p><p></p> The conserved pyridoxal 5'-phosphate binding site is boxed. An asterisk (*) is used to indicate the two catalytic residues, K40 and Y263. Arrows indicate eight conserved amino acid residues constituting the entryway to the active site [11]

SDS-polyacrylamide gel electrophoresis of alanine racemase from (Lanes B-D) and protein molecular weight markers (Lane A, BioRad Dual Color Marker) stained with Coomassie blue

<p><b>Copyright information:</b></p><p>Taken from "Purification and preliminary crystallization of alanine racemase from "</p><p>http://www.biomedcentral.com/1471-2180/7/40</p><p>BMC Microbiology 2007;7():40-40.</p><p>Published online 17 May 2007</p><p>PMCID:PMC1885262.</p><p></p> Lanes B-D represent the three peak fractions from the final gel filtration step

Summary of the major NTDs and malaria in the ASEAN countries.

<p>Summary of the major NTDs and malaria in the ASEAN countries.</p

NTDs of the ASEAN Countries according to WHO PCT data and other sources.

<p>SAC: school-age children, preSAC: pre-school age children.</p><p>NTDs of the ASEAN Countries according to WHO PCT data and other sources.</p

Detection of Viruses By Counting Single Fluorescent Genetically Biotinylated Reporter Immunophage Using a Lateral Flow Assay

We demonstrated a lateral flow immunoassay (LFA) for detection of viruses using fluorescently labeled M13 bacteriophage as reporters and single-reporter counting as the readout. AviTag-biotinylated M13 phage were functionalized with antibodies using avidin–biotin conjugation and fluorescently labeled with AlexaFluor 555. Individual phage bound to target viruses (here MS2 as a model) captured on an LFA membrane strip were imaged using epi-fluorescence microscopy. Using automated image processing, we counted the number of bound phage in micrographs as a function of target concentration. The resultant assay was more sensitive than enzyme-linked immunosorbent assays and traditional colloidal-gold nanoparticle LFAs for direct detection of viruses

The Countries of ASEAN.

<p>*Calculated by multiplying the proportion of people living below the different poverty levels by the total population</p><p>The Countries of ASEAN.</p

Additional file 1: Table S1. of The hookworm Ancylostoma ceylanicum intestinal transcriptome provides a platform for selecting drug and vaccine candidates

The 100 most abundant proteins in A. ceylanicum intestine. (PDF 326 kb

Sensitive Detection of Norovirus Using Phage Nanoparticle Reporters in Lateral-Flow Assay

<div><p>Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 10⁷ virus-like particles per mL, one hundred-fold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair.</p></div