Recruitment of participants.

<p>Recruitment of participants.</p

Baseline characteristics.

<p>Mann Whitney U tests were performed; all data are expressed as means±SD.</p

Body composition and resting energy expenditure (measured by BIA and indirect calorimetry, respectively).

<p>Mann Whitney U tests were performed; all data are expressed as means±SD; *indicates statistical significance.</p

Salivary cortisol, urinary catecholamines and dopamine, and plasma metanephrines and 12-hour excretion rates of urinary metanephrines.

<p>Mann Whitney U tests were performed; all data are expressed as means±SD; *indicates statistical significance.</p

Parameters of sleep continuity and sleep architecture (polysomnographic study).

<p>Mann Whitney U tests were performed; all data are expressed as means±SD; *indicates statistical significance.</p

Midnight and morning salivary cortisol concentrations in insomniacs and controls.

<p>Midnight values were significantly higher in insomniacs than in controls (p = 0.02), whereas morning values did not differ significantly (p = 0.18).</p

Recruitment of participants.

<p>Recruitment of participants.</p

Ubiquitous Cks1 overexpression in the bone marrow is not sufficient to induce myeloproliferative disease or leukemia.

<p>4×10⁶ GFP-positive cells were transplanted with an infection rate of 30%. <b>A</b>, Cks1 mRNA levels in the indicated bone marrow subsets of transplanted mice were analyzed by realtime PCR at day 160 after transplantation. Shown is the relative expression of Cks1 as compared to the B cell expression of the GFP mice as a control sample set at 1. <i>GFP</i> group: n = 2; <i>Cks1-GFP</i> group: n = 3. The bars represent the mean relative expression ± standard deviation. realtime PCR was performed in duplicates. <b>B</b>, Immunoblot analysis of the bone marrow of transplanted mice on the day of indisposition and sacrifice. <b>C</b>, The left panel shows the GFP positivity of the bone marrow (BM) and spleen (S). The three right panels show the percentages of B-, T- and myeloid cells. <b>D</b>, Representative flow cytometric dot blot images of the myeloid cell compartment of <i>GFP</i>, <i>Cks1-GFP</i> and <i>Myc-GFP</i> animals on the day of their sacrifice. <b>E</b>, Survival analysis of lethally irradiated recipient mice that were transplanted with <i>GFP</i>, <i>Cks1-GFP</i> or <i>Myc-GFP</i> infected bone marrow. None of the <i>GFP</i> or <i>Cks1-GFP</i> mice died of leukemia, while all <i>Myc-GFP</i> mice succumbed to AML. Median survival: <i>GFP</i> group, 469 days; <i>Cks1-GFP</i> group, 541.5 days; <i>Myc-GFP</i> group, 52 days. Only the survival of <i>Myc-GFP</i> group is significantly different from the other groups.</p