Spatial organization and stoichiometry of N-terminal domain-mediated glycosyltransferase complexes in Golgi membranes determined by fret microscopy

The functional link between glycolipid glycosyltransferases (GT) relies on the ability of these proteins to form organized molecular complexes. The organization, stoichiometry and composition of these complexes may impact their sorting properties, sub-Golgi localization, and may determine relative efficiency of GT in different glycolipid biosynthetic pathways. In this work, by using Förster resonance energy transfer microscopy in live CHO-K1 cells, we investigated homo- and hetero-complex formation by different GT as well as their spatial organization and molecular stoichiometry on Golgi membranes. We find that GalNAcT and GalT2 Ntd are able to form hetero-complexes in a 1:2 molar ratio at the trans-Golgi network and that GalT2 but not GalNAcT forms homo-complexes. Also, GalNAcT/GalT2 complexes exhibit a stable behavior reflected by its clustered lateral organization. These results reveals that particular topological organization of GTs may have functional implications in determining the composition of glycolipids in cellular membranes

Infectiousness of Sylvatic and Synanthropic Small Rodents Implicates a Multi-host Reservoir of Leishmania (Viannia) braziliensis

Submitted by Adagilson Silva ([email protected]) on 2017-09-15T18:49:59Z No. of bitstreams: 1 26448187 2015 and-inf.oa.pdf: 409773 bytes, checksum: 86f9fa8878143d50fff9aaa5d5cfab39 (MD5)Approved for entry into archive by Adagilson Silva ([email protected]) on 2017-09-15T19:47:18Z (GMT) No. of bitstreams: 1 26448187 2015 and-inf.oa.pdf: 409773 bytes, checksum: 86f9fa8878143d50fff9aaa5d5cfab39 (MD5)Made available in DSpace on 2017-09-15T19:47:18Z (GMT). No. of bitstreams: 1 26448187 2015 and-inf.oa.pdf: 409773 bytes, checksum: 86f9fa8878143d50fff9aaa5d5cfab39 (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, BrasilThe possibility that a multi-host wildlife reservoir is responsible for maintaining transmission of Leishmania (Viannia) braziliensis causing human cutaneous and mucocutaneous leishmaniasis is tested by comparative analysis of infection progression and infectiousness to sandflies in rodent host species previously shown to have high natural infection prevalences in both sylvatic or/and peridomestic habitats in close proximity to humans in northeast Brazil

Tectono-Stratigraphic Evolution of the Atuel Depocenter During the Late Triassic to Early Jurassic Rift Stage, Neuquén Basin, West-Central Argentina

The Neuquén basin presents an almost continuous record from the Late Triassic until the Paleocene,making it an excellent case study of the most relevant tectonic stages of southern South America during the Mesozoic. It was initiated in the Late Triassic to Early Jurassic times as a continental rift basin in the context of a widespread extensional stage that affected western Gondwana andculminated with the break-up of the supercontinent.The Atuel depocenter is located in the northern sector of the Neuquén basin. Synrift and sag units are represented by Upper Triassic to Lower Jurassic siliciclastic marine and continental sedimentary rocks including the oldest marine deposits of the basin, of Late Triassic age. The depocenter infill hasbeen deformed and exhumed during the Andean orogeny, being presently exposed in the northern sector of the Malargüe fold and thrust belt. In this review, we have integrated a large set of stratigraphic, sedimentologic, geochronologic and structural data in order to unravel the tectono-sedimentary evolution of the Atuel depocenter, and to evaluate the main controlling factors of thesynrift stage. We analyzed data from the synrift units, such as facies and thickness distribution, sandstone provenance, detrital zircon geochronology data, kinematic data from outcrop scale normal faults, angular and progressive unconformities and subsurface information. Reactivation of preexisting NNW-striking anisotropies under a regional NNE extension resulted in anoblique rift setting, which generated a bimodal distribution of NNW- and WNW-striking major normal faults. Strain and stress tensors obtained from the kinematic and dynamic analysis of structural data show a complex heterogeneity that we interpreted as a result of local stress permutations due to both activity of the larger faults, and to strain partitioning inside the Atueldepocenter.Sedimentologic and petrographic data revealed a complex evolution with strong lateral variations of the depositional environments during the synrift phase, which lasted from Rhaetian to Pliensbachian times. We identified several stages that were controlled by processes of initiation, propagation, growth, linkage and deactivation of new and reactivated faults along the depocenter evolution, in combination with sea level changes related to global eustatic variations. Sandstone provenance data suggest that an important basin reorganization by the Toarcian, probably related to the initiation of the sag stage in this depocenter.Fil: Bechis, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Diversidad Cultural y Procesos de Cambio. Universidad Nacional de Río Negro. Instituto de Investigaciones en Diversidad Cultural y Procesos de Cambio; ArgentinaFil: Giambiagi, Laura Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Museo de Historia Natural de San Rafael - Ianigla | Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Museo de Historia Natural de San Rafael - Ianigla | Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Museo de Historia Natural de San Rafael - Ianigla; ArgentinaFil: Tunik, Maisa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación en Paleobiología y Geología; ArgentinaFil: Suriano, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Museo de Historia Natural de San Rafael - Ianigla | Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Museo de Historia Natural de San Rafael - Ianigla | Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Museo de Historia Natural de San Rafael - Ianigla; ArgentinaFil: Lanés, Silvia. Cape Town; SudáfricaFil: Mescua, Jose Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Museo de Historia Natural de San Rafael - Ianigla | Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Museo de Historia Natural de San Rafael - Ianigla | Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Museo de Historia Natural de San Rafael - Ianigla; Argentina. Universidad Nacional de Cuyo; Argentin