Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium

Present investigation deals with optimization of appropriate substrate concentration and incubation temperature both for growth of Saccharomyces cerevisiae and invertase production. Submerged fermentation technique was employed in the present study. The maximal production of invertase during the course of study was achieved after 48 h of incubation using initial sucrose concentration, 15.0 g l-1. The sugar consumption and dry cell mass were also examined. Data was subjected to kinetic analysis and on the basis of kinetic parameters such as Yp/x (amount of enzyme produced mg-1 cell mass), Yp/s (amount of enzyme produced mg-1 sugar consumed), Yx/s (mg cells mg-1 substrate consumed), Ys/x (mg sugar consumed mg-1 cell mass produced), qp (Amount of enzyme produced mg-1 sugar consumed h-1), qs (mg mg-1 cells h-1), qx (mg cells mg-1 sugar consumed h-1), µ (mg cells produced h-1), it was found that temperature had a direct influence both on substrate consumption and synthesis of enzyme. Similarly, higher concentrations of sucrose in fermentation medium induced catabolite repression of yeast invertase

Towards the Automatic Classification of Documents in User-generated Classifications

There is a huge amount of information scattered on the World Wide Web. As the information flow occurs at a high speed in the WWW, there is a need to organize it in the right manner so that a user can access it very easily. Previously the organization of information was generally done manually, by matching the document contents to some pre-defined categories. There are two approaches for this text-based categorization: manual and automatic. In the manual approach, a human expert performs the classification task, and in the second case supervised classifiers are used to automatically classify resources. In a supervised classification, manual interaction is required to create some training data before the automatic classification task takes place. In our new approach, we intend to propose automatic classification of documents through semantic keywords and building the formulas generation by these keywords. Thus we can reduce this human participation by combining the knowledge of a given classification and the knowledge extracted from the data. The main focus of this PhD thesis, supervised by Prof. Fausto Giunchiglia, is the automatic classification of documents into user-generated classifications. The key benefits foreseen from this automatic document classification is not only related to search engines, but also to many other fields like, document organization, text filtering, semantic index managing

Why Civil Service Reforms Do Not Work

Public sector reform (PSR) efforts in developing countries have been less than successful in the past. Motivated by budgetary considerations, they have focused on downsizing and procedural changes without radically altering the outmoded incentive system, which, in many countries, is now characterised by declining real wages, wage compression, and a non-merit promotion and reward system. Using results from the incentives literature, this paper argues that, for a reform effort to succeed, public sector human resource management (HRM) will have to be reformed at an early stage to establish productivity incentives in the public sector. These will include introducing substantial autonomy to organisations in their work, incentive schemes, and HRM along the lines of the now well-accepted concept of central bank independence. Past PSR efforts have also attempted to conduct a unified reform effort led centrally by the ministry of finance. A continuous process like PSR—spread out over a considerable period and involving many different people and organisations—might need to build in decentralisation, local leadership and local incentives, and HRM. PSR must be based on the recognition that people are at the heart of public service. As a result, managing human resources must be at the centre of any effort. The people who are at the center of this change can either be its architects and beneficiaries or its losers and therefore opponents of change. Design and implementation of reforms must, therefore, be sensitive to this important fact. It is essential that the reform is led by individuals at the organisation level who understand the vision as well as process of change. Governments must empower such leadership to guide, initiate, innovate, and manage change.Civil Services, Reforms

Why Civil Service Reforms Do Not Work

Public sector reform (PSR) efforts in developing countries have been less than successful in the past. Motivated by budgetary considerations, they have focused on downsizing and procedural changes without radically altering the outmoded incentive system, which, in many countries, is now characterised by declining real wages, wage compression, and a non-merit promotion and reward system. Using results from the incentives literature, this paper argues that, for a reform effort to succeed, public sector human resource management (HRM) will have to be reformed at an early stage to establish productivity incentives in the public sector. These will include introducing substantial autonomy to organisations in their work, incentive schemes, and HRM along the lines of the now well-accepted concept of central bank independence. Past PSR efforts have also attempted to conduct a unified reform effort led centrally by the ministry of finance. A continuous process like PSRspread out over a considerable period and involving many different people and organisationsmight need to build in decentralisation, local leadership and local incentives, and HRM. PSR must be based on the recognition that people are at the heart of public service. As a result, managing human resources must be at the centre of any effort. The people who are at the center of this change can either be its architects and beneficiaries or its losers and therefore opponents of change. Design and implementation of reforms must, therefore, be sensitive to this important fact. It is essential that the reform is led by individuals at the organisation level who understand the vision as well as process of change. Governments must empower such leadership to guide, initiate, innovate, and manage change.Civil Services, Reforms

Awake the Sleeper Within : Releasing the Energy of Stifled Domestic Commerce!

Policy in Pakistan has been fairly path-dependant, placing a higher weight on export promotion and domestic industrialisation development than on domestic commerce. Yet domestic commerce is growing rapidly, and quite possibly is the largest sector in the economy. This paper argues that a more holistic policy, with no favourites, that allows for all sectors to grow leads to better long-term economic results. A vibrant domestic commerce sector is the core of the economy facilitating intermediation between supply and demand, entrepreneurial development, risk-taking, innovation, and competitive markets. Such an economy moves beyond commodity exports to brand name, process, and capital exports, all of which command a higher rate of return. Pakistan could therefore achieve a higher and a more sustainable growth rate by adopting a more balanced growth strategy.domestic commerce, policy

Impact of Export Subsidies on Pakistan’s Exports

Throughout Pakistans history, policy has sought to promote exports through government support and incentives. The government machinery is geared to export promotion especially through direct and indirect subsidies. Surprisingly, these policies have been continued without serious examination. This paper makes a first attempt to evaluate these policies by estimating the impact of two such schemesexport financing and rebate/refund schemeson export performance. Our analysis shows that, over the long run, the export financing scheme had a negative effect on exports while the rebate/refund scheme affected exports insignificantly. Subsidy schemes clearly do not seem to work, yet they have been retained for many years.Rebate, Duty Drawback, Export Financing, exports, trade, exchange rate, co-integration, Vector Error Correction, Pakistan

Beyond Planning and Mercantilism: An Evaluation of Pakistan’s Growth Strategy

Through the nineties Pakistan remained preoccupied with crisis management. All debate and policy was, as a result, involved with current policy and our coping with the IMF programmes. Adjustment was the main theme leaving little room for growth initiatives.1 A lively debate has raged on the distributional impacts of adjustment policy on which the government and the thinking community have adopted opposing stances, often with much emotion. With this focus of economic and political discussion on critiquing of the current government and its policies, there has been little effort put in understanding and reviewing the country’s growth strategy. This paper attempts to assess the evolution of Pakistan’s long-term growth strategy.2 It is my contention that the growth strategy remains inertia-ridden because of the lack of an academic community and debate.3 The paper will also attempt to identify the actors who influence and shape this strategy. This will be followed by what changes should be made in that strategy, based on more recent developments in economic thinking and experience in the world. For long-run sustained growth that will lead us to join the club of the more advanced countries, a new strategy based on the latest research findings will be needed. Finally, I shall point to the factors that impede the adoption of such a strategy, and especially to our owning such a strategy.Economic Growth

Financial Capacity and Willingness of Farmers to pay for Irrigation Services in the Post-reform Scenario in Pakistan: Two Case Studies

To eliminate the subsidy on the canal irrigation system, the Government of Pakistan has decided to reform the management, intending to make it more efficient, equitable, transparent, and able to take care of the sustainability of the world’s largest contiguous irrigation network. The water users are being entrusted with greater role in the management through the formation of Farmers’ Organisations (FOs) to operate and maintain secondary canals and pay for full cost of water delivery. Ultimate payer will be the farmer. The economic viability of the reforms, therefore, much depends on farmers’ ability and willingness to pay for the cost of irrigation water delivery, which is expected to rise. This paper estimates financial liabilities of the farmers in the post-reform scenario, and assesses their capacity and willingness to pay for liabilities in the provinces of Punjab and Sindh. One distributary in each of the two provinces is studied as the reference distributary, where FOs have already been formed. The cost of desired level of operation and maintenance levels are worked out using secondary data for 1997-98. With these costs, the water users in the Punjab and Sindh provinces need to pay Rs 333 and Rs 373 per ha for their water service, respectively. The estimated O&M costs form about 5.4 and 3 percent of production costs and 3.8 and 3.5 percent of the net income in the Punjab and Sindh provinces respectively. The farmers’ net income from crop enterprise is higher than the cost of water. Thus, an average farmer has the potential to pay for water. Recent experience of Hakra 4-R Distributary FO suggests that the farmers are also willing to pay for water service, if they are organised properly.