33 research outputs found
Isolated of a vitamin D-dependent, calcium-binding protein from brush borders of rat duodenal mucosa
Effects of amitraz on cytochrome P-450 dependent monooxygenases and estrogenic activity in MCF-7 human breast cancer cells and immature female rats
This study investigated the ability of amitraz、a formamidine insecticide、to induce cytochrome P450-dependent monooxygenases
and to disrupt estrogenic activity in human breast cancer MCF-7 cells and immature female rats. In MCF-7 cells、treatment with 10
lM amitraz for 24 h increased 7-ethoxyresorufin O-deethylase activity in cell homogenate. Treatment of MCF-7 cells with 1 and 10
lM amitraz for 3 h replaced previously bound [3H]17b-estradiol (E2) from estrogen receptors. Treatment with 0.1 and 1 lM amitraz
for 2 days inhibited [3H]thymidine incorporation into the DNA of MCF-7 cells while the inhibition was blocked in cells co-treated
with 1 nM E2 and amitraz. In immature female rats、treatment with 50 mg/kg amitraz intraperitoneally for 3 days increased cytochrome
P450 content、7-ethoxyresorufin、methoxyresorufin and pentoxyresorufin O-dealkylases、and benzo[a]pyrene hydroxylase
activities in liver microsomes. The results of immunoblot analysis revealed that amitraz induced liver microsomal CYP1A1/2,
2B1/2B2、and 3A proteins. Treatment with 10 and 25 mg/kg amitraz for 3 days dose-dependently decreased uterine weight and peroxidase
activity in immature female rats while the decreases were blocked in rats co-treated with 10 lg/kg E2 and 10 or 25 mg/kg
amitraz. These in vitro and in vivo findings suggest that amitraz induces multiple forms of P450 and exerts weak antiestrogenic
activity