\u3cem\u3eASI1\u3c/em\u3e, a Gene Encoding a Novel Leucine Zipper Protein, Is Induced during Development of the Macronucleus in \u3cem\u3eTetrahymena\u3c/em\u3e

Sexual reproduction in the ciliate Tetrahymena follows a complex developmental program involving the sequential regulation of dozens of genes. Genes that are up-regulated during post-zygotic development in Tetrahymena were isolated by subtractive hybridization. Anlagen stage induced gene 1 (ASI1) encodes a 2.8 kb transcript that contains a single intron and is induced during macronuclear development. ASI1 is a single copy gene in both the micronucleus and the macronucleus. It encodes a 95 kDa conceptual protein with a leucine zipper near the amino terminu

Progeny of Germ Line Knockouts of \u3cem\u3eASI2\u3c/em\u3e, a Gene Encoding a Putative Signal Transduction Receptor in \u3cem\u3eTetrahymena Thermophila\u3c/em\u3e, Fail to Make the Transition from Sexual Reproduction to Vegetative Growth

The ciliated protozoan Tetrahymena has two nuclei: a germ line micronucleus and a somatic macronucleus. The transcriptionally active macronucleus has about 50 copies of each chromosome. At sexual reproduction (conjugation), the parental macronucleus is degraded and new macronucleus develops from a mitotic product of the zygotic micronucleus. Development of the macronucleus involves massive genome remodeling, including deletion of about 6000 specific internal eliminated sequences (IES) and multiple rounds of DNA replication. A gene encoding a putative signal transduction receptor, ASI2, (anlagen stage induced 2) is up-regulated during development of the new macronuclei (anlagen). Macronuclear ASI2 is nonessential for vegetative growth. Homozygous ASI2 germ line knockout cells with wild type parental macronuclei proceed through mating but arrest at late macronuclear anlagen development and die before the first post-conjugation fission. IES elimination occurs in these cells. Two rounds of postzygotic DNA replication occur normally in progeny of ASI2 germ line knockouts, but endoreduplication of the macronuclear genome is arrested. The germ line ASI2 null phenotype is rescued in a mating of a knockout strain with wild type cells

Upgrading a Social Media Strategy to Increase Twitter Engagement During the Spring Annual Meeting of the American Society of Regional Anesthesia and Pain Medicine.

Microblogs known as tweets are a rapid, effective method of information dissemination in health care. Although several medical specialties have described their Twitter conference experiences, Twitter-related data in the fields of anesthesiology and pain medicine are sparse. We therefore analyzed the Twitter content of 2 consecutive spring meetings of the American Society of Regional Anesthesia and Pain Medicine using publicly available online transcripts. We also examined the potential contribution of a targeted social media campaign on Twitter engagement during the conferences. The original Twitter meeting content was largely scientific in nature and created by meeting attendees, the majority of whom were nontrainee physicians. Physician trainees, however, represent an important and increasing minority of Twitter contributors. Physicians not in attendance predominantly contributed via retweeting original content, particularly picture-containing tweets, and thus increased reach to nonattendees. A social media campaign prior to meetings may help increase the reach of conference-related Twitter discussion

Implementasi Teknik Maze Untuk Mengembangkan Kreativitas Dan Kemampuan Kognitif Anak Kelompok B2 TK Shanti Kumara III Sempidi Mengwi Badung

Penelitian ini bertujuan untuk meningkatkan kreativitas dan kemampuan kognitif anak pada kelompok B2 TK Shanti Kumara III Sempidi Mengwi Badung. Pelaksanaan penelitian didasarkan pada kelompok observasi awal yang menunjukkan bahwa krativitas anak masih rendah. Oleh karena itu, diperlukan penerapan teknik maze untuk meningkatkan kreativitas anak. Subjek penelitian adalah 26 anak TK Shanti Kumara III Sempidi Mengwi Badung. Analisis data menggunakan teknik deskripsi kuantitatif. Hasil penelitian menunujukkan bahwa kualitas kreativitas dan kemampuan kognitif anak melalui penerapan teknik maze pada anak mengalami peningkatan. Secara rinci dapat dilihat dari 1) skor kemampuan kognitif anak meningkat di siklus I sampai dengan siklus II sebesar 46,15% dan 2) skor kreativitas anak meningkat di siklus I sampai dengan siklus II sebesar 53,85%. Berdasarkan penelitian tersebut Penerapan teknik maze dapat digunakan sebagai salah satu alternatif teknik yang menarik dalam pembelajaran di TK untuk meningkatkan kreativitas anak.Kata Kunci : kemampuan kognitif, kreativitas, teknik maze This research aims at improving children's creativity and cognitive skill of B2 Group at TK Shanti Kumara III Sempidi Mengwi Badung. The research was based on pre-observation which showed that the children's creativity was low. Thus, maze technique was needed to improve children's creativity. The subject was 26 children at Shanti Kumara III Kindergarten Sempidi Mengwi Badung. Data analysis using quantitative descriptive technique. The result of the research shows that 1) cognitive skill of children increased on cycle I and was continued until cycle II of 46.15%, 2) creativity score of children also increased on cycle I and was continued until cycle II of 53.85%. Based on those results, it is concluded that the implementation of Maze Technique can be used effectively as an interesting alternative technique in kindergarten learning process to improve children creativity

Tuberculosis in children in India-II: Chemotherapy for tuberculosis

Tubercle bacilli readily become resistant to the common drugs, and resistant bacilli are more likely to proliferate if they are present in the patient at the start of treatment. So always use more than one drug. The only possible exception is prophylaxis for an asymptomatic case with a normal X-ray. CAUTION! (1) Never give intermittent (twice or thrice weekly) treatment unless every dose can be supervised by a health worker. Daily treatment is usually mandatory. (2) When you give more than one drug, give them both at the same time, so that high blood levels coincide; do not give one drug daily and the other drug less often. THE DOSES of the commonly used drugs for daily and intermittent treatment in children and adults are: lsoniazid (H) 5 mg/kg/24 hours if he is moderately ill and 10 mg/kg/24 hours if he is severely ill. The dose for a twice weekly course is 15 mg/kg. CAUTION! Opinions on the dose of isoniazid vary. Some consider 10 mg/kg/24 hours too much for an Indian child and always give 5 mg. Rifampicin (R) 10 mg/kg/24 hours, or 10 mg/kg twice weekly. Pyrazinamide (Z) 35 mg/kg/24 hours, 75 mg/kg twice weekly or 50 mg/kg thrice weekly, is an important drug for short course treatment, so try to include it whenever it is mentioned in the regimes below. Streptomycin (S) 10-20 mg/kg/24 hours, or 40 mg/kg twice weekly, to a total of not more than 0.75 g. Streptomycin is painful, so avoid it if you can. If you give it, inject in different places each day, because repeated injections into the same site are painful. Ethambutol (E) 25 mg/kg/24 hours for 2 months, then 15 mg/kg/24 hours. Avoid ethambutol in younger children (under 12); they are unable to complain of the early symptoms of retrobulbar neuritis (blindness). Thiacetazone (T) 4 mg/kg/24 hours to a maximum Of 150 mg; unsuitable for intermittent treatment

A Content Analysis of Jihadist Magazines: Theoretical Perspectives

During its violent spread across the Middle East, the Islamic State of Iraq and Sham (ISIS) amassed both a local and international following in large part due to its usage of emergent media distribution. Beginning in 2014, ISIS’s Ministry of Media published an English-language magazine, Dabiq, disseminating its issues through online platforms. Dabiq and its successor Rumiyah both serve as propagandistic recruitment material for ISIS’s international community as well as broadcasting the message of the jihadist movement to ISIS’s enemies. This study analyzed ISIS’s publications using a qualitative content analysis in order to identify jihadist recruitment strategies through the perspectives of agenda-setting theory, the diffusion of innovations, symbolic convergence theory, and speech codes theory. These communication theories characterize the roles that civilizational conflict, population demographics, narrative themes, and emergent media play in the diffusion of the jihadist movement. This study samples the textual content and imagery of issues of Dabiq and Rumiyah, using thematic analysis to procedurally code the data by recognizing shared characteristics and concepts. The fundamental goal of this study is to gain a greater understanding of the way ISIS, its members, and the jihadist movement communicate their intentions, with the hope of preventing further recruitment and radicalization. The two following research questions drive this study: (1) What themes are present in the ISIS publications of Dabiq and Rumiyah? (2) How do the themes of these publications vary over time

Tuberculosis in children in India-I

Tuberculosis is different in children. It involves many organs, instead of being the predominantly respiratory disease that it usually is in adults. Fortunately, it readily responds to treatment–if you diagnose it early enough and treat it for long enough! This is the problem. Unfortunately, tuberculosis causes such non-specific symptoms and signs, and you are so seldom able to isolate bacilli, that you may never be sure of the diagnosis. Even experts sometimes disagree. In India particularly, it is a disease of the poorest of the poor, but even in them it causes only a small proportion of their burden of morbidity. The great problem is to reach those infected. Of every thousand Indians, seven children and about twenty adults have active tuberculosis, and five of these adults are sputum positive. Only about half the 9 million in the community at any one time are ever diagnosed, and of these only about 13% complete their treatment, so there is a huge pool of infectious cases, half a million of whom die each year. Fortunately, the incidence of tuberculosis among children reporting to hospital is slowly decreasing, probably largely due to improved coverage with BCG

Power Management in Mobile Computing (a Survey)

Rapid advances in technology have resulted in laptop (mobile) computers with performance and features comparable to desktop (stationary) machines. Advances in rechargeable battery technology have failed to keep pace, decreasing the usefulness of mobile computers and portable wireless devices. Several methods of power management can be used to prolong the battery life of a mobile computer. We provide a detailed analysis of power consumption typically encountered in a networked laptop computer and the power management methods currently used. We also outline some novel proposed power management methods

VENUS: A Virtual Environment Network Using Satellites

Virtual environment (VE) designs have evolved from text-based to immersive graphical systems. The next logical step of this evolution is to have a fully immersive environment in which thousands of widely distributed users will be able to move around and interact. This requires a VE architecture that can scale well for a large number of participants while providing the necessary support for quality of service, security and flexibility. Current VE architectures are unable to fully meet these requirements and a new network/protocol architecture is needed. The VENUS approach addresses these problems by creating a network architecture which is scalable and flexible. We define a new architecture consisting of a transmit-only satellite/server and bi-directional links which will be capable of sustaining a wide-area virtual environment. We then offer the preliminary results of our experiments

Clinical and laboratory variability in a cohort of patients diagnosed with type 1 VWD in the United States

Von Willebrand disease (VWD) is the most common inherited bleeding disorder, and type 1 VWD is the most common VWD variant. Despite its frequency, diagnosis of type 1 VWD remains the subject of much debate. In order to study the spectrum of type 1 VWD in the United States, the Zimmerman Program enrolled 482 subjects with a previous diagnosis of type 1 VWD without stringent laboratory diagnostic criteria. VWF laboratory testing and full length VWF gene sequencing were performed for all index cases and healthy control subjects in a central laboratory. Bleeding phenotype was characterized using the ISTH Bleeding Assessment Tool. At study entry, 64% of subjects had VWF:Ag or VWF:RCo below the lower limit of normal, while 36% had normal VWF levels. VWF sequence variations were most frequent in subjects with VWF:Ag < 30 IU/dL (82%) while subjects with type 1 VWD and VWF:Ag ≥ 30 IU/dL had an intermediate frequency of variants (44%). Subjects whose VWF testing was normal at study entry had a similar rate of sequence variations as the healthy controls at 14% of subjects. All subjects with severe type 1 VWD and VWF:Ag ≤ 5 IU/dL had an abnormal bleeding score, but otherwise bleeding score did not correlate with VWF:Ag level. Subjects with a historical diagnosis of type 1 VWD had similar rates of abnormal bleeding scores compared to subjects with low VWF levels at study entry. Type 1 VWD in the United States is highly variable, and bleeding symptoms are frequent in this population