\u3cem\u3eASI1\u3c/em\u3e, a Gene Encoding a Novel Leucine Zipper Protein, Is Induced during Development of the Macronucleus in \u3cem\u3eTetrahymena\u3c/em\u3e

Sexual reproduction in the ciliate Tetrahymena follows a complex developmental program involving the sequential regulation of dozens of genes. Genes that are up-regulated during post-zygotic development in Tetrahymena were isolated by subtractive hybridization. Anlagen stage induced gene 1 (ASI1) encodes a 2.8 kb transcript that contains a single intron and is induced during macronuclear development. ASI1 is a single copy gene in both the micronucleus and the macronucleus. It encodes a 95 kDa conceptual protein with a leucine zipper near the amino terminu

A Content Analysis of Jihadist Magazines: Theoretical Perspectives

During its violent spread across the Middle East, the Islamic State of Iraq and Sham (ISIS) amassed both a local and international following in large part due to its usage of emergent media distribution. Beginning in 2014, ISIS’s Ministry of Media published an English-language magazine, Dabiq, disseminating its issues through online platforms. Dabiq and its successor Rumiyah both serve as propagandistic recruitment material for ISIS’s international community as well as broadcasting the message of the jihadist movement to ISIS’s enemies. This study analyzed ISIS’s publications using a qualitative content analysis in order to identify jihadist recruitment strategies through the perspectives of agenda-setting theory, the diffusion of innovations, symbolic convergence theory, and speech codes theory. These communication theories characterize the roles that civilizational conflict, population demographics, narrative themes, and emergent media play in the diffusion of the jihadist movement. This study samples the textual content and imagery of issues of Dabiq and Rumiyah, using thematic analysis to procedurally code the data by recognizing shared characteristics and concepts. The fundamental goal of this study is to gain a greater understanding of the way ISIS, its members, and the jihadist movement communicate their intentions, with the hope of preventing further recruitment and radicalization. The two following research questions drive this study: (1) What themes are present in the ISIS publications of Dabiq and Rumiyah? (2) How do the themes of these publications vary over time

Power Management in Mobile Computing (a Survey)

Rapid advances in technology have resulted in laptop (mobile) computers with performance and features comparable to desktop (stationary) machines. Advances in rechargeable battery technology have failed to keep pace, decreasing the usefulness of mobile computers and portable wireless devices. Several methods of power management can be used to prolong the battery life of a mobile computer. We provide a detailed analysis of power consumption typically encountered in a networked laptop computer and the power management methods currently used. We also outline some novel proposed power management methods

Progeny of Germ Line Knockouts of \u3cem\u3eASI2\u3c/em\u3e, a Gene Encoding a Putative Signal Transduction Receptor in \u3cem\u3eTetrahymena Thermophila\u3c/em\u3e, Fail to Make the Transition from Sexual Reproduction to Vegetative Growth

The ciliated protozoan Tetrahymena has two nuclei: a germ line micronucleus and a somatic macronucleus. The transcriptionally active macronucleus has about 50 copies of each chromosome. At sexual reproduction (conjugation), the parental macronucleus is degraded and new macronucleus develops from a mitotic product of the zygotic micronucleus. Development of the macronucleus involves massive genome remodeling, including deletion of about 6000 specific internal eliminated sequences (IES) and multiple rounds of DNA replication. A gene encoding a putative signal transduction receptor, ASI2, (anlagen stage induced 2) is up-regulated during development of the new macronuclei (anlagen). Macronuclear ASI2 is nonessential for vegetative growth. Homozygous ASI2 germ line knockout cells with wild type parental macronuclei proceed through mating but arrest at late macronuclear anlagen development and die before the first post-conjugation fission. IES elimination occurs in these cells. Two rounds of postzygotic DNA replication occur normally in progeny of ASI2 germ line knockouts, but endoreduplication of the macronuclear genome is arrested. The germ line ASI2 null phenotype is rescued in a mating of a knockout strain with wild type cells

VENUS: A Virtual Environment Network Using Satellites

Virtual environment (VE) designs have evolved from text-based to immersive graphical systems. The next logical step of this evolution is to have a fully immersive environment in which thousands of widely distributed users will be able to move around and interact. This requires a VE architecture that can scale well for a large number of participants while providing the necessary support for quality of service, security and flexibility. Current VE architectures are unable to fully meet these requirements and a new network/protocol architecture is needed. The VENUS approach addresses these problems by creating a network architecture which is scalable and flexible. We define a new architecture consisting of a transmit-only satellite/server and bi-directional links which will be capable of sustaining a wide-area virtual environment. We then offer the preliminary results of our experiments

Implementasi Teknik Maze Untuk Mengembangkan Kreativitas Dan Kemampuan Kognitif Anak Kelompok B2 TK Shanti Kumara III Sempidi Mengwi Badung

Penelitian ini bertujuan untuk meningkatkan kreativitas dan kemampuan kognitif anak pada kelompok B2 TK Shanti Kumara III Sempidi Mengwi Badung. Pelaksanaan penelitian didasarkan pada kelompok observasi awal yang menunjukkan bahwa krativitas anak masih rendah. Oleh karena itu, diperlukan penerapan teknik maze untuk meningkatkan kreativitas anak. Subjek penelitian adalah 26 anak TK Shanti Kumara III Sempidi Mengwi Badung. Analisis data menggunakan teknik deskripsi kuantitatif. Hasil penelitian menunujukkan bahwa kualitas kreativitas dan kemampuan kognitif anak melalui penerapan teknik maze pada anak mengalami peningkatan. Secara rinci dapat dilihat dari 1) skor kemampuan kognitif anak meningkat di siklus I sampai dengan siklus II sebesar 46,15% dan 2) skor kreativitas anak meningkat di siklus I sampai dengan siklus II sebesar 53,85%. Berdasarkan penelitian tersebut Penerapan teknik maze dapat digunakan sebagai salah satu alternatif teknik yang menarik dalam pembelajaran di TK untuk meningkatkan kreativitas anak.Kata Kunci : kemampuan kognitif, kreativitas, teknik maze This research aims at improving children's creativity and cognitive skill of B2 Group at TK Shanti Kumara III Sempidi Mengwi Badung. The research was based on pre-observation which showed that the children's creativity was low. Thus, maze technique was needed to improve children's creativity. The subject was 26 children at Shanti Kumara III Kindergarten Sempidi Mengwi Badung. Data analysis using quantitative descriptive technique. The result of the research shows that 1) cognitive skill of children increased on cycle I and was continued until cycle II of 46.15%, 2) creativity score of children also increased on cycle I and was continued until cycle II of 53.85%. Based on those results, it is concluded that the implementation of Maze Technique can be used effectively as an interesting alternative technique in kindergarten learning process to improve children creativity

Upgrading a Social Media Strategy to Increase Twitter Engagement During the Spring Annual Meeting of the American Society of Regional Anesthesia and Pain Medicine.

Microblogs known as tweets are a rapid, effective method of information dissemination in health care. Although several medical specialties have described their Twitter conference experiences, Twitter-related data in the fields of anesthesiology and pain medicine are sparse. We therefore analyzed the Twitter content of 2 consecutive spring meetings of the American Society of Regional Anesthesia and Pain Medicine using publicly available online transcripts. We also examined the potential contribution of a targeted social media campaign on Twitter engagement during the conferences. The original Twitter meeting content was largely scientific in nature and created by meeting attendees, the majority of whom were nontrainee physicians. Physician trainees, however, represent an important and increasing minority of Twitter contributors. Physicians not in attendance predominantly contributed via retweeting original content, particularly picture-containing tweets, and thus increased reach to nonattendees. A social media campaign prior to meetings may help increase the reach of conference-related Twitter discussion

Progeny of germ line knockouts of ASI2, a gene encoding a putative signal transduction receptor in Tetrahymena thermophila, fail to make the transition from sexual reproduction to vegetative growth

AbstractThe ciliated protozoan Tetrahymena has two nuclei: a germ line micronucleus and a somatic macronucleus. The transcriptionally active macronucleus has about 50 copies of each chromosome. At sexual reproduction (conjugation), the parental macronucleus is degraded and new macronucleus develops from a mitotic product of the zygotic micronucleus. Development of the macronucleus involves massive genome remodeling, including deletion of about 6000 specific internal eliminated sequences (IES) and multiple rounds of DNA replication. A gene encoding a putative signal transduction receptor, ASI2, (anlagen stage induced 2) is up-regulated during development of the new macronuclei (anlagen). Macronuclear ASI2 is nonessential for vegetative growth. Homozygous ASI2 germ line knockout cells with wild type parental macronuclei proceed through mating but arrest at late macronuclear anlagen development and die before the first post-conjugation fission. IES elimination occurs in these cells. Two rounds of postzygotic DNA replication occur normally in progeny of ASI2 germ line knockouts, but endoreduplication of the macronuclear genome is arrested. The germ line ASI2 null phenotype is rescued in a mating of a knockout strain with wild type cells

Evaluation of Methicillin-Resistant Staphylococcus aureus Carriage and High Livestock Production Areas in North Carolina through Active Case Finding at a Tertiary Care Hospital

: Recent reports from the Netherlands document the emergence of novel multilocus sequence typing (MLST) types (e.g., ST-398) of methicillin-resistant Staphylococcus aureus (MRSA) in livestock, particularly swine. In Eastern North Carolina (NC), one of the densest pig farming areas in the United States, as many as 14% of MRSA isolates from active case finding in our medical center have no matches in a repetitive sequence-based polymerase chain reaction (rep-PCR) library. The current study was designed to determine if these non-matched MRSA (NM-MRSA) were geographically associated with exposure to pig farming in Eastern NC. While residential proximity to farm waste lagoons lacked association with NM-MRSA in a logistic regression model, a spatial cluster was identified in the county with highest pig density. Using MLST, we found a heterogeneous distribution of strain types comprising the NM-MRSA isolates from the most pig dense regions, including ST-5 and ST-398. Our study raises the warning that patients in Eastern NC harbor livestock associated MRSA strains are not easily identifiable by rep-PCR. Future MRSA studies in livestock dense areas in the U.S. should investigate further the role of pig–human interactions