Thyroidal angiogenesis in zebrafish (Danio rerio) exposed to high perchlorate concentration

WOS: 000266174000050As a well known environmental contaminant, perchlorate inhibits thyroidal iodide uptake and reduces thyroid hormone levels. In zebrafish (Danio rerio) exposed to high concentrations of sodium perchlorate (200, 350 and 500 mg/L) for 10 days, remarkable angiogenesis was identified, not only histopathologically but also statistically by counting of the small, medium and large sized (grades 1 to 3) vessels per unit area. Angiogenic response is concluded as the most sensitive parameter for rating of high concentrations of perchlorate exposure

Cardiac toxicity of acrolein exposure in embryonic zebrafish (Danio rerio)

Arman, Sezgi/0000-0002-4247-0639WOS: 000527523900002PubMed: 32307682Acrolein is a widely distributed pollutant produced from various sources such as industrial waste, organic combustion, and power plant emissions. It is also intentionally released into irrigation canals to control invasive aquatic plants. Zebrafish (Danio rerio) has a good reputation for being an attractive model organism for developmental and toxicological research. in this study, zebrafish embryos were exposed to acrolein to investigate the cardiotoxic effects. the 96-h LC50 (median lethal concentration) value of acrolein was determined as 654.385 mu g/L. Then, the embryos were treated with the sublethal experimental concentrations of acrolein (1, 4, 16, 64, and 256 mu g/L) for 96 h. Embryos were examined at 48, 72, and 96 h post-fertilization (hpf). Acrolein affected the cardiac morphology and function of the embryos. Sinus venosus-bulbus arteriosus (SV-BA) distance of 64 mu g/L and 256 mu g/L acrolein groups was elongated compared with the control samples. Immunostaining with MF20 antibody clearly exhibited that the atrium positioned posterior to the ventricle which indicated cardiac looping inhibition. Histological preparations also showed the mispositioning and the lumens of the chambers narrowed. Acrolein-induced increased heart rate was noted in the 4, 16, 64, and 256 mu g/L treatment groups. Taken together, these results indicated that acrolein disrupted the heart development and cardiac function in zebrafish, suggesting that its water-borne risks should be considered seriously

Histochemical Characterization of Convict Cichlid (Amatitlania nigrofasciata) Intestinal Goblet Cells

In the present study, some histochemical features of goblet cells of the intestine of convict cichlid (A. nigrofasciata) were described. In order to reveal the main histological construction, transverse sections of different parts of intestine were firstly stained with hematoxylin eosin (H-E). Sections of anterior, mid and posterior intestinal segments were also treated with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated. Goblet cells of anterior intestine were colored strongly by AB (pH 2.5), AF and PAS; and moderately by PAS/AB (pH 2.5) (AB dominant). In the mid part of the gastrointestinal tract, goblet cells were also stained strongly with AB, PAS and PAS/AB (pH 2.5) (AB dominant), however, their reactions to KOH/PAS treatment were recorded as negative. Because of numerous supranuclear vacuoles of the epithelial cells, only a few goblet cells could be differentiated in posterior parts of alimentary channel, with their weakly reaction to AB (pH 2.5), PAS and PAS/AB (pH 2.5). Moreover, these cells were not stained with AF and KOH/PAS. According to their affinities, goblet cells oriented in anterior and the mid intestine were mainly classified as acidic (AB-positive) and neutral (PAS-positive). Statistical analysis were confirmed that, the numbers of acidic and neutral cells of per unite square of epithelial area were significantly different

Atresia and Apoptosis in Preovulatory Follicles in the Ovary of Danio rerio (Zebrafish)

WOS: 000274072200014Although apoptosis, the programmed cell death, is the main mechanism in follicular atresia and especially postovulatory regression in mammals, its role in fish ovary is still controversial. In order to discuss the possible functions of atresia as a context of apoptosis, the preovulatory follicles of the freshwater teleost, Danio rerio (zebrafish) were investigated by light microscopy. The main characteristics of atresia of vitellogenic oocytes of zebrafish were determined as a significant hypertrophy and hyperplasia of the granulosa cells. The theca cells also became slightly hyperplasic while the zona radiata was degenerated and the basal membrane located between granulosa and theca was also disintegrated. The aggregation of compacted chromatin in a crescent pattern, and cell fragmentation that indicates to formation of apoptotic bodies were evaluated as the morphological evidences for apoptosis in granulosa cells of the oocytes of zebrafish. Towards the end of atresia, the vitellus is fully degenerated and absorbed, some apoptotic bodies were also observed between the translucent clusters of theca cells. Apoptotic process was still continued when an atrium had been formed.Ege University Research CouncilEge University [Fen 003]This study is partly based on MSc thesis of the second author. Preparation of manuscript was supported by Ege University Research Council 2000 Grant No. Fen 003

Histochemical Characterization of Convict Cichlid (Amatitlania nigrofasciata) Intestinal Goblet Cells

WOS: 000405162300003In the present study, some histochemical features of goblet cells of the intestine of convict cichlid (A. nigrofasciata) were described. In order to reveal the main histological construction, transverse sections of different parts of intestine were firstly stained with hematoxylin eosin (H-E). Sections of anterior, mid and posterior intestinal segments were also treated with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated. Goblet cells of anterior intestine were colored strongly by AB (pH 2.5), AF and PAS; and moderately by PAS/AB (pH 2.5) (AB dominant). In the mid part of the gastrointestinal tract, goblet cells were also stained strongly with AB, PAS and PAS/AB (pH 2.5) (AB dominant), however, their reactions to KOH/PAS treatment were recorded as negative. Because of numerous supranuclear vacuoles of the epithelial cells, only a few goblet cells could be differentiated in posterior parts of alimentary channel, with their weakly reaction to AB (pH 2.5), PAS and PAS/AB (pH 2.5). Moreover, these cells were not stained with AF and KOH/PAS. According to their affinities, goblet cells oriented in anterior and the mid intestine were mainly classified as acidic (AB-positive) and neutral (PAS-positive). Statistical analysis were confirmed that, the numbers of acidic and neutral cells of per unite square of epithelial area were significantly different

The Effects of Dioctyl Adipate (DOA) on The Liver Histology of Labidochromis caeruleus Fryer, 1956 (Cichlidae, Teleostei)

WOS: 000281800400004Although dioctyl adipate (DOA. C(22)H(42)O(4)) is one of the most common plasticizers that are used for polymerization, the ecotoxicological effects of it is not well known. From this point of view, aim of this preliminary investigation was to analyze the histopathological changes in the liver of Labidochromis caeruleus (yellow princess), exposed to 0.75 ppm DOA for 30 days. When compared to controls, the most prominent changes occurred in the DOA exposed group were noted as a significant steatosis (fat deposisiton), fibrosis and expanding necrotic areas. The dilatation of sinusoids and central veins, poikilocytosis, and hemorrhage were also observed. The data presented in this study shows that. DOA is a hepatotoxic chemical at least for Labidocromis caerulous