6 research outputs found
Recovery of sperm after epididymal refrigeration from domestic cats using ACP-117c and Tris extenders
Uso do diluente água de coco em pó (ACP-103®) na conservação prolongada do sêmen do varrão: avaliação in vitro e in vivo
Refrigeração do epidÃdimo canino a 4ºC e recuperação dos espermatozoides epididimários utilizando ACP-106c
USO DE DILUENTES E TEMPERATURAS ALTERNATIVAS NA CONSERVAÇÃO PROLONGADA DO SÊMEN DO VARRÃO
The use of appropriate extenders is important for the success of an artificial insemination program. The objective of this study was to evaluate the efficiency of alternative extenders for swine semen at different temperatures (17 to 10 °C). The following extenders were used: Beltsville Thawing Solution (BTS), powdered coconut water (ACP-103®), and skimmed milk powder (LPD). The 50 ejaculates were analyzed daily, in natura and after dilution, during the 5-day period of semen preservation (D0 to D4), regarding spermatic vigor and motility. Acrosome integrity and sperm viability were evaluated on D0 and D4. Data were analyzed using Mann-Whitney, Students, Tukey and chi-square tests (p<0.05). The LPD extender at 10 °C presented higher motility and sperm vigor compared to BTS and ACP until D2, and to treatments stored at 17 °C. Acrosome vitality and integrity remained higher (p<0.001) with LPD at 10 °C on D0 and D4. LPD showed to be a good extender for the swine semen at lower temperature (10 °C). Furthermore, it provided better protection to sperm cells, by allowing greater integrity and vitality of the acrosome