9 research outputs found
DNA fragmentation of MDA-MB 231 breast cancer cells analysed in 1% agarose gel after 24 hours incubation with different concentration of Artonin E.
<p>Lanes 1–3 represents 3, 10 and 30 μM of Artonin E, lane 4 is untreated cancer cells, lane 5 is positive control well treated with 4μg/mL camptothecin and lane 6 is a 1kilobase DNA ladder.</p
MDA-MB 231 breast cancer cell cycle regulation after 12 hours treatment with (B) 3 μM, (C) 10 μM, and (D) 30 μM Artonin E.
<p>(A) is untreated control. (E) Analysis of cell population in the cycle phases. Values are mean ± standard deviation of three replicates. *Means in each cell cycle phase significantly (p<0.05) different from the control.</p
Western blot analysis of Artonin E (3, 10, and 30 μM) treated MDA-MB231 breast cancer cells after 24 hours.
<p>(A) and the levels of protein expression quantified from the western blotting analysis of Artonin E treated MDA-MB 231 cells using Bio-rad Image Lab software (B).</p
The percentage of MDA-MB-231 cell line viability after treatment with Artonin E.
<p>The experiment was done in triplicate at 24, 48, and 72 hours and each point are presented as mean ± SD.</p
The accession number and sequence of the primers used in the gene expression studies.
<p>The accession number and sequence of the primers used in the gene expression studies.</p
Mechanism of action of Artonin E in MDA-MB 231 breast cancer cells.
<p>Mechanism of action of Artonin E in MDA-MB 231 breast cancer cells.</p
Acridine orange and propidium iodide double staining of MDA-MB 231 breast cancer cells after 24 hour exposure.
<p>(A) Control, (B) 3 μM Artonin E. (C) 10 μM Artonin E, (D) 30 μM Artonin E and (E) Quantification of apoptotic morphology at 24 and 48 hours. Each result is presented as mean ± SD of three replicates. * indicates significant difference from the control of each phase (p<0.05). VC = Viable cells; BL = Cell membrane blebbing; CC = chromatin condensation; EA = Early apoptosis; LA = late apoptosis; MN = marginated nuclear chromatin; SN = secondary necrosis. Magnification X200.</p
Apoptosis pathway- and cell cycle-related genes and protein expression in Artonin E treated MDA-MB 231 cells.
<p>Apoptosis pathway- and cell cycle-related genes and protein expression in Artonin E treated MDA-MB 231 cells.</p