5 research outputs found
Determination of tocopherols and sitosterols in seeds and nuts by QuEChERS-liquid chromatography
[EN]In the present work a simple, reliable and affordable sample treatment method for the simultaneous
analysis of tocopherols and free phytosterols in nuts was developed. Analyte extraction was carried
out using the QuEChERS methodology and analyte separation and detection were accomplished using
HPLC–DAD. The use of this methodology for the extraction of natural occurring substances provides
advantages such as speed, simplicity and ease of use. The parameters evaluated for the validation of
the method developed included the linearity of the calibration plots, the detection and quantification limits, repeatability, reproducibility and recovery. The proposed method was successfully applied to the
analysis of tocopherols and free phytosterols in samples of almonds, cashew nuts, hazelnuts, peanuts,
tiger nuts, sun flower seeds and pistachio