Detecting truth in suspect interviews: the effect of use of evidence (early and gradual) and time delay on Criteria-Based Content Analysis, Reality Monitoring and inconsistency within suspect statements

The strategic use of evidence in interviews with suspects has been shown to increase the ability of interviewers to accurately and consistently distinguish truthful from deceptive accounts. The present study considers the effect of early and gradual revelation of evidence by the interviewer, and the effect of shorter and longer delay on the verbal quality of truth-teller and liar statements within a mock crime paradigm. It was hypothesised that gradual disclosure of evidence (1) in terms of inconsistencies (a) within statements and (b) between statements and such evidence and (2) of the criteria of Criteria-Based Content Analysis (CBCA) and of Reality Monitoring (RM) would emphasise differences in the verbal quality of truth-teller and liar statements. Forty-two high school students took part in the study. The use of statement-evidence and within-statement inconsistency appears to be a robust cue to deception across interview style and delay. This indicates that gradual disclosure in interviews may increase interviewer accuracy in veracity decisions by eliciting statement inconsistencies. However, gradual revelation and delay affected the ability of CBCA and RM criteria to distinguish the veracity of suspect statements.N/

Sicherheit durch Erziehung und Bildung - notwendig, wirksam, guenstig

Copy held by FIZ Karlsruhe; available from UB/TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Unmasking of autoreactive CD4 T cells by depletion of CD25 regulatory T cells in systemic lupus erythematosus

Objective Autoreactive CD4 T cells specific for nuclear peptide antigens play an important role in tolerance breakdown during the course of systemic lupus erythematosus (SLE). However, reliable detection of these cells is limited due to their low frequency in peripheral blood. The authors assess autoreactive CD4 T cells in a representative SLE collective (n=38) by flow cytometry and study the influence of regulatory T cells (Treg) on their antigenic challenge. Methods CD4 T-cell responses were determined according to intracellular CD154 expression induced after 6-h short-term in-vitro stimulation with the SLE-associated autoantigen SmD1(83-119). To clarify the influence of Treg on the activation of autoreactive CD4 T cells, CD25 Treg were depleted by magnetic activated cell sorting before antigen-specific stimulation in selected experiments. Results In the presence of Treg, autoreactive CD4 T-cell responses to SmD1(83-119) were hardly observable. However, Treg removal significantly increased the frequency of detectable SmD1(83-119)-specific CD4 T cells in SLE patients but not in healthy individuals. Consequently, by depleting Treg the percentage of SmD1(83-119)-reactive SLE patients increased from 18.2% to 63.6%. This unmasked autoreactivity of CD4 T cells correlated with the disease activity as determined by the SLE disease activity index (p=0.005*, r=0.779). Conclusions These data highlight the pivotal role of the balance between autoreactive CD4 T cells and CD25 Treg in the dynamic course of human SLE. Analysing CD154 expression in combination with a depletion of CD25 Treg, as shown here, may be of further use in approaching autoantigen-specific CD4 T cells in SLE and other autoimmune diseases

Urinary CD4 T cells identify SLE patients with proliferative lupus nephritis and can be used to monitor treatment response

OBJECTIVES: Proliferative lupus nephritis (LN) is one of the major concerns in the treatment of systemic lupus erythematosus (SLE). Here we evaluate urinary CD4 T cells as a biomarker of active LN and indicator of treatment response. METHODS: Urinary CD3CD4 T cells were quantified using flow cytometry in 186 urine samples from 147 patients with SLE. Fourteen patients were monitored as follow-up. Thirty-one patients with other nephropathies and 20 healthy volunteers were included as controls. RESULTS: In SLE, urinary CD4 T cell counts >/=800/100 ml were observed exclusively in patients with active LN. Receiver operator characteristic analysis documented clear separation of SLE patients with active and non-active LN (area under the curve 0.9969). All patients with up-to-date kidney biopsy results showing proliferative LN had high urinary CD4 T cell numbers. In patients monitored under therapy, normalisation of urinary CD4 T cell counts indicated lower disease activity and better renal function. In contrast, patients with persistence of, or increase in, urinary T cells displayed worse outcomes. CONCLUSIONS: Urinary CD4 T cells are a highly sensitive and specific marker for detecting proliferative LN in patients with SLE. Furthermore, monitoring urinary CD4 T cells may help to identify treatment responders and treatment failure and enable patient-tailored therapy in the future