41 research outputs found
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Effects of hemorrhagic shock, aspirin, and ethanol on secretagogue-induced experimental pancreatitis
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A simple and efficient method of measuring in vitro amylase secretion by dispersed pancreatic acini
A method of measuring amylase secretion from dispersed pancreatic acini is described that utilizes a 20-micron nylon mesh to separate cell-associated from secreted amylase. When compared with the standard centrifugation assay, the mesh technique permits easy measurement at multiple, closely spaced time points while reducing the number of samples generated and allowing rapid manipulation of the extracellular suspending medium
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Failure of a potent cholecystokinin antagonist to protect against diet-induced pancreatitis in mice
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Effects of a choline-deficient ethionine-supplemented diet on phospholipase C activity in mouse pancreatic acinar cell membranes and in electropermeabilized mouse pancreatic acini
Pancreatic duct obstruction in rabbits causes digestive zymogen and lysosomal enzyme colocalization.
The pancreatic duct of anesthetized rabbits was cannulated and, in some animals, flow of pancreatic exocrine secretions was blocked by raising the cannula to a vertical position. Blockage for 3-7 h caused a rapid and significant rise in serum amylase activity and an increase in amylase activity within the pancreas. The concentration of lysosomal enzymes in the pancreas was not altered but they became redistributed among subcellular fractions and, as a result, an increased amount was recovered in the 1,000-g, 15-min pellet, which was enriched in zymogen granules. Immunofluorescence studies indicated that lysosomal enzymes become localized within organelles which, in size and distribution, resemble zymogen granules. They also contain digestive enzyme zymogens. Blockage of pancreatic secretions also caused lysosomal enzyme-containing organelles to become more fragile and subject to in vitro rupture. These changes noted after short-term pancreatic duct obstruction are remarkably similar to those previously noted to occur during the early stages of diet and secretagogue-induced experimental pancreatitis, observations that have suggested that colocalization of digestive enzyme zymogens and lysosomal hydrolases might result in intracellular digestive enzyme activation and be an important early event in the evolution of those forms of experimental acute pancreatitis