Effectiveness of Dentin Bonding Agents

The interface between a composite resin restoration and dentin is still a problem with regard to secondary caries and esthetics. With conventional restorative techniques the absence of bonding between the restorative material and the dentin produces marginal gaps. These gaps are populated with microorganisms or trap pigments. Therefore, adhesive techniques are required for functional and esthetic restorations. In extracted teeth stored in 0.1% thymol solution, 8 cylindrical cavities (diameter: 3 mm; depth: 2 mm) and 8 class V cavities (1/2 of the margin in dentin) were prepared in each group and filled with composite resin. Before actual placement the following dentin adhesives were used: Scotchbond 2 (3M), Gluma (Bayer), Scotchbond LC (3M), Dentin Adhesit (Ivoclar), Dentin Adhesive (Kulzer) and Durafill-Bond (Kulzer) as a control. Before and after thermocycling (TC) (2000 cycles, 5° C to 55° C) replicas were taken and a quantitative margin analysis in the SEM was performed at 200 x magnification. To analyze the data, a rating scale (four criteria) was used to characterize the marginal configuration. Statistical analysis of the results showed that the materials Gluma, Scotchbond LC and Scotchbond 2 yielded significantly (p \u3c 0.05) better margins than the other materials in both cylindrical and class V cavities. Before TC values \u3e 90% were found with Gluma, Scotchbond LC and Scotchbond 2 in Class V cavities. However, after TC, these values decreased significantly to 66% for Gluma, 42% for Scotchbond LC and 81% for Scotchbond 2. Scotchbond 2 showed approximately 80% excellent margins in both cavity forms after TC. A subsequent clinical study should be done to confirm these favourable results in vivo

Quantitative Margin Analysis in the Scanning Electron Microscope.

Interface between restorative materials and tooth hard substances must be morphologically as perfect as possible to avoid plaque accumulation and subsequent secondary caries or pulpal diseases. Therefore the marginal behavior of restorations is an important parameter to predict their longevity. Morphologically, the quality of margins is characterized by different well defined criteria. Using a replica technique it is possible to assess the complete marginal circumference of restorations in the SEM. Margins of restorations show a large variety of their morphology. This publication describes a method to quantify the quality of dental restorations. The restoration margins are traced on the SEM screen with a digitizer and an interface to measure the margin\u27s length. Simultaneously the margin quality is assessed and assigned to the corresponding lengths. The % distribution of the quality criteria for each restoration is then calculated. Using a comparative light microscope, the replicas are aligned and mounted identically in the SEM for longitudinal studies. The results presented are limited to tests for the accuracy of the method. Using 5 criteria to characterize the margin quality, it was found that the difference between two measurements by the same operator, 4 weeks apart was 3% ± 2.6%. The largest difference for one group was 9%. In another accuracy test where 4 criteria for margin characterization were used, the difference between two measurements was 1.9% ± 0.9%. The largest difference between two groups found was 3.4%. This method can be used for longitudinal studies in vivo, but also for in vitro screening tests with new materials

DBEndo: a web-based endodontic case management tool

BACKGROUND: The success of endodontic treatment depends-among many other factors-on good documentation. Paper-based records are often difficult to read or incomplete and commercially available tools focus on billing. An electronic record captures the state of treatment at all times. Databases are a common tool in everyday life. RESULTS: Here, we present a database created for the Charite-Universitatsmedizin Berlin, Germany. Through consistent digital documentation, data analytics of patients, root canal anatomies, instrumentation techniques, efficacy of chemical disinfection, root filling techniques, and corresponding recall success rates, which needed extensive research before, are now easy to perform. Tables and even graphics and data analystics are only one click away and can be exported to other programs. CONCLUSIONS: DBEndo is a database to store and visualise internally, as well as to share endodontic cases online. For academic use we provide the database including all forms and some anonymous data for free at: http://dbendo.charite.de . Through easy import and export of the data, the system is open and flexible

TACAN is an essential component of the mechanosensitive ion channel responsible for pain sensing

Mechanotransduction, the conversion of mechanical stimuli into electrical signals, is a fundamental process underlying several physiological functions such as touch and pain sensing, hearing and proprioception. This process is carried out by specialized mechanosensitive ion channels whose identities have been discovered for most functions except pain sensing. Here we report the identification of TACAN (Tmem120A), an essential subunit of the mechanosensitive ion channel responsible for sensing mechanical pain. TACAN is expressed in a subset of nociceptors, and its heterologous expression increases mechanically-evoked currents in cell lines. Purification and reconstitution of TACAN in synthetic lipids generates a functional ion channel. Finally, knocking down TACAN decreases the mechanosensitivity of nociceptors and reduces behavioral responses to mechanical but not to thermal pain stimuli, without affecting the sensitivity to touch stimuli. We propose that TACAN is a pore-forming subunit of the mechanosensitive ion channel responsible for sensing mechanical pain

Who Eats Whom in a Pool? A Comparative Study of Prey Selectivity by Predatory Aquatic Insects

Predatory aquatic insects are a diverse group comprising top predators in small fishless water bodies. Knowledge of their diet composition is fragmentary, which hinders the understanding of mechanisms maintaining their high local diversity and of their impacts on local food web structure and dynamics. We conducted multiple-choice predation experiments using nine common species of predatory aquatic insects, including adult and larval Coleoptera, adult Heteroptera and larval Odonata, and complemented them with literature survey of similar experiments. All predators in our experiments fed selectively on the seven prey species offered, and vulnerability to predation varied strongly between the prey. The predators most often preferred dipteran larvae; previous studies further reported preferences for cladocerans. Diet overlaps between all predator pairs and predator overlaps between all prey pairs were non-zero. Modularity analysis separated all primarily nectonic predator and prey species from two groups of large and small benthic predators and their prey. These results, together with limited evidence from the literature, suggest a highly interconnected food web with several modules, in which similarly sized predators from the same microhabitat are likely to compete strongly for resources in the field (observed Pianka’s diet overlap indices >0.85). Our experiments further imply that ontogenetic diet shifts are common in predatory aquatic insects, although we observed higher diet overlaps than previously reported. Hence, individuals may or may not shift between food web modules during ontogeny

The MLL recombinome of acute leukemias in 2017

Chromosomal rearrangements of the human MLL/KMT2A gene are associated with infant, pediatric, adult and therapy-induced acute leukemias. Here we present the data obtained from 2345 acute leukemia patients. Genomic breakpoints within the MLL gene and the involved translocation partner genes (TPGs) were determined and 11 novel TPGs were identified. Thus, a total of 135 different MLL rearrangements have been identified so far, of which 94 TPGs are now characterized at the molecular level. In all, 35 out of these 94 TPGs occur recurrently, but only 9 specific gene fusions account for more than 90% of all illegitimate recombinations of the MLL gene. We observed an age-dependent breakpoint shift with breakpoints localizing within MLL intron 11 associated with acute lymphoblastic leukemia and younger patients, while breakpoints in MLL intron 9 predominate in AML or older patients. The molecular characterization of MLL breakpoints suggests different etiologies in the different age groups and allows the correlation of functional domains of the MLL gene with clinical outcome. This study provides a comprehensive analysis of the MLL recombinome in acute leukemia and demonstrates that the establishment of patient-specific chromosomal fusion sites allows the design of specific PCR primers for minimal residual disease analyses for all patients

The MLL recombinome of acute leukemias in 2017

Chromosomal rearrangements of the human MLL/KMT2A gene are associated with infant, pediatric, adult and therapy-induced acute leukemias. Here we present the data obtained from 2345 acute leukemia patients. Genomic breakpoints within the MLL gene and the involved translocation partner genes (TPGs) were determined and 11 novel TPGs were identified. Thus, a total of 135 different MLL rearrangements have been identified so far, of which 94 TPGs are now characterized at the molecular level. In all, 35 out of these 94 TPGs occur recurrently, but only 9 specific gene fusions account for more than 90% of all illegitimate recombinations of the MLL gene. We observed an age-dependent breakpoint shift with breakpoints localizing within MLL intron 11 associated with acute lymphoblastic leukemia and younger patients, while breakpoints in MLL intron 9 predominate in AML or older patients. The molecular characterization of MLL breakpoints suggests different etiologies in the different age groups and allows the correlation of functional domains of the MLL gene with clinical outcome. This study provides a comprehensive analysis of the MLL recombinome in acute leukemia and demonstrates that the establishment of patient-specific chromosomal fusion sites allows the design of specific PCR primers for minimal residual disease analyses for all patients.</p