miRTarBase update 2014: an information resource for experimentally validated miRNA-target interactions

MicroRNAs (miRNAs) are small non-coding RNA molecules capable of negatively regulating gene expression to control many cellular mechanisms. The miRTarBase database (http://mirtarbase.mbc.nctu.edu.tw/) provides the most current and comprehensive information of experimentally validated miRNA-target interactions. The database was launched in 2010 with data sources for >100 published studies in the identification of miRNA targets, molecular networks of miRNA targets and systems biology, and the current release (2013, version 4) includes significant expansions and enhancements over the initial release (2010, version 1). This article reports the current status of and recent improvements to the database, including (i) a 14-fold increase to miRNA-target interaction entries, (ii) a miRNA-target network, (iii) expression profile of miRNA and its target gene, (iv) miRNA target-associated diseases and (v) additional utilities including an upgrade reminder and an error reporting/user feedback system

Collective Translation as Forking (分岔)

Together with a Taiwanese working group, we have been producing a Chinese translation of Aesthetic Programming: A Handbook of Software Studies, a free and open source book first written by Winnie Soon and Geoff Cox in 2020, and released in English in a git repository, dynamic website, downloadable PDF and printed form. Apart from learning to code in p5.js, the book addresses the cultural and aesthetic dimensions of programming from its insides, as a means to think and act critically, and to understand the importance of programming as a cultural practice that can develop discussion of issues that are relatively under-acknowledged in technical subjects such as gender, race and sexuality. Importantly, the book is understood as a computational object, not released as a fixed and universal teaching resource, but rather a situated curriculum with the potential for extension and customization with other arts and coding communities. The use of Git has allowed the authors to formalize its production as an iterative process, allowing for reversioning and for others to fork a copy and customize with different references, examples, critical reflections and even new chapters. The interest is in forking a book like forking software, and incorporating local knowledge and examples, and how this resonates with a politics of cultural translation. This essay will elaborate on the process of running two open participatory workshops that were conducted in Taipei and London (in 2023) with the aim to challenge some of the normative social relations of production associated with translation, and explore other possibilities of collective practice. The politics of translation has been well-established in general, but what of the specifics of translating a book such as this? Aside from the technical and aesthetic challenges and implications, this raises the question of how the Chinese language model enforces particular hegemonic worldviews that occlude differences. With all the variants of Chinese language, how is this tied to expressions of colonial power that resonates with our use of English? Given the rich variations of Chinese and indigenous languages (not least in a Taiwanese context), we are curious how we might be sensitive to language diversity that challenges the Western-centrism of programming in English (and inherent nationalisms). We are also mindful of the way that “queer” politics has informed the way that terms can be appropriated/expropriated, as a means to “talk back” to the source codes of oppression. What are the implications of drawing the practices of forking and translating together

THE EFFECT OF COUNTER MOVEMENT JUMP PERFORMANCE IN MIDDLEAGED ELDERLY PRACTICING TAI-CHI EXERCISE

The purpose of this study was to investigate biomechanical effects of Tal-Chi exercise on the lower-extremity in middle-aged elders during counter-movement jump. Twelve middle-aged elders with regular Tai Chi exercise experience and twelve healthy middle-aged elders participated in this study. Ten Vicon Motion System cameras, two Kistler force plates were used simultaneously to capture the kinematic and dynamlc parameters of standing vertical jumps. Independent samples &test was performed for statistical analysis ( u = .05 ). Since the jump height of Tai Chi group was significantly higher ( p c .05 ). It showed that practicing Tai Chi exercise could effectively slow down the degeneration of the moment and power at the hip Joint. Therefore, middle-aged elders were recommended to engage in long-term Tai Chi exercise

Use of electroporation and reverse iontophoresis for extraction of transdermal multibiomarkers

Congo Tak-Shing Ching1,2, Lin-Shien Fu3-5, Tai-Ping Sun1, Tzu-Hsiang Hsu1, Kang-Ming Chang21Department of Electrical Engineering, National Chi Nan University, Puli, Nantou County, 2Department of Photonics and Communication Engineering, Asia University, Wufeng, Taichung, 3Department of Pediatrics, National Yang Ming University, Taipei, 4Institute of Technology, National Chi Nan University, Puli, 5Department of Pediatrics, Taichung Veterans General Hospital, Taichung City, TaiwanBackground: Monitoring of biomarkers, like urea, prostate-specific antigen (PSA), and osteopontin, is very important because they are related to kidney disease, prostate cancer, and ovarian cancer, respectively. It is well known that reverse iontophoresis can enhance transdermal extraction of small molecules, and even large molecules if reverse iontophoresis is used together with electroporation. Electroporation is the use of a high-voltage electrical pulse to create nanochannels within the stratum corneum, temporarily and reversibly. Reverse iontophoresis is the use of a small current to facilitate both charged and uncharged molecule transportation across the skin. The objectives of this in vitro study were to determine whether PSA and osteopontin are extractable transdermally and noninvasively and whether urea, PSA, and osteopontin can be extracted simultaneously by electroporation and reverse iontophoresis.Methods: All in vitro experiments were conducted using a diffusion cell assembled with the stratum corneum of porcine skin. Three different symmetrical biphasic direct currents (SBdc), five various electroporations, and a combination of the two techniques were applied to the diffusion cell via Ag/AgCl electrodes. The three different SBdc had the same current density of 0.3 mA/cm2, but different phase durations of 0 (ie, no current, control group), 30, and 180 seconds. The five different electroporations had the same pulse width of 1 msec and number of pulses per second of 10, but different electric field strengths of 0 (ie, no voltage, control group), 74, 148, 296, and 592 V/cm. Before and after each extraction experiment, skin impedance was measured at 20 Hz.Results: It was found that urea could be extracted transdermally using reverse iontophoresis alone, and further enhancement of extraction could be achieved by combined use of electroporation and reverse iontophoresis. Conversely, PSA and osteopontin were found to be extracted transdermally only by use of reverse iontophoresis and electroporation with a high electrical field strength (>296 V/cm). After application of reverse iontophoresis, electroporation, or a combination of the two techniques, a reduction in skin impedance was observed.Conclusion: Simultaneous transdermal extraction of urea, PSA, and osteopontin is possible only for the condition of applying reverse iontophoresis in conjunction with high electroporation.Keywords: electroporation, reverse iontophoresis, nanochannels, noninvasive, urea, prostate-specific antigen, osteoponti

Knowledge-Enriched Visual Storytelling

Stories are diverse and highly personalized, resulting in a large possible output space for story generation. Existing end-to-end approaches produce monotonous stories because they are limited to the vocabulary and knowledge in a single training dataset. This paper introduces KG-Story, a three-stage framework that allows the story generation model to take advantage of external Knowledge Graphs to produce interesting stories. KG-Story distills a set of representative words from the input prompts, enriches the word set by using external knowledge graphs, and finally generates stories based on the enriched word set. This distill-enrich-generate framework allows the use of external resources not only for the enrichment phase, but also for the distillation and generation phases. In this paper, we show the superiority of KG-Story for visual storytelling, where the input prompt is a sequence of five photos and the output is a short story. Per the human ranking evaluation, stories generated by KG-Story are on average ranked better than that of the state-of-the-art systems. Our code and output stories are available at https://github.com/zychen423/KE-VIST.Comment: AAAI 202

Using Barthel Index and Performance Status Scale to predict inpatient bowel preparation quality

Purpose: Bowel preparation, cecal intubation rate, and adenoma detection rate influence the quality of colonoscopy and interval cancer rate. Adequate bowel preparation is first and essential step for not only procedure safety but also lesions defection ability. According to literature, several factors affect inpatient bowel preparation such as age, socioeconomic class, opiate/ tricyclic antidepressants use, and physical status classification system by American society of anesthesiologists. However, there was still lack of general predicting model. Under hypothesis that patient s activity may be related to bowel movement then influence the bowel preparation. Thus we tried to predict inpatient bowel preparation by activity scoring system: Barthel index and performance status scale by eastern cooperative oncology group that routinely assessed in our inpatient nursing work. This study was aimed to evaluate predicting power of Barthel index and performance status scale by eastern cooperative oncology group for inpatient bowel preparation. We also investigated the cutoff values of scoring systems for inadequate bowel preparation. Methods: All consecutive patients hospitalized for colonoscopy at the gastroenterology ward between 2016 May to 2016 June were retrospectively analyzed. We used Aronchick scale for bowel preparation evaluation. The level excellent and good were defined as adequate; fair and poor were defined as inadequate. All patients activity statuses were assessed by Barthel index and performance status scale by eastern cooperative oncology group. The results were tested by receiver operating characteristic curve. Final the cutoffs were calculated by Youden\u27s index. Results: During the period of study, 100 hospitalized patients were retrospectively reviewed. The adequate bowel preparation rate was 70%. In addition, adenoma detection rate was 35%; cecal intubation rate was 99%. In receiver operating characteristic curve, area under curve of Barthel index was 0.798; area under curve of performance status scale by eastern cooperative oncology group was 0.824. Both of them were good discrimination for bowel preparation. After Youden\u27s index calculation, we found 82 was cutoff for Barthel index and 1 was for performance status scale by eastern cooperative oncology group. Conclusion: Both Barthel index and performance status scale by eastern cooperative oncology group were valuable to predicting inpatient bowel preparation. In daily practice, cutoffs let medical team pay more attention to possible inadequate bowel preparation

Evaluation of Antioxidant and Free Radical Scavenging Capacities of Polyphenolics from Pods of Caesalpinia pulcherrima

Thirteen polyphenolics were isolated from fresh pods of Caesalpinia pulcherrima using various methods of column chromatography. The structures of these polyphenolics were elucidated as gallic acid (1), methyl gallate (2), 6-O-galloyl-d-glucoside (3), methyl 6-O-galloyl-β-d-glucoside (4), methyl 3,6-di-O-galloyl-α-d-glucopyranoside (5), gentisic acid 5-O-α-d-(6′-O-galloyl)glucopyranoside (6), guaiacylglycerol 4-O-β-d-(6′-O-galloyl)glucopyranoside (7), 3-methoxy-4-hydroxyphenol 1-O-β-d-(6′-O-galloyl) glucopyranoside (8), (+)-gallocatechin (9), (+)-catechin (10), (+)-gallocatechin 3-O-gallate (11), myricetin 3-rhamnoside (12), and ampelopsin (13). All isolated compounds were tested for their antioxidant activities in the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and peroxynitrite radicals scavenging assays. Among those compounds, 11, 12, and 2 exhibited the best DPPH-, hydroxyl-, and peroxynitrite radical-scavenging activities, respectively. Compound 7 is a new compound, and possesses better scavenging activities towards DPPH but has equivalent hydroxyl radical scavenging activity when compared to BHT. The paper is the first report on free radical scavenging properties of components of the fresh pods of Caesalpinia pulcherrima. The results obtained from the current study indicate that the free radical scavenging property of fresh pods of Caesalpinia pulcherrima may be one of the mechanisms by which this herbal medicine is effective in several free radical mediated diseases