Response of deep-water agglutinated foraminifera to dysoxic conditions in the California Borderland basins

Analysis of agglutinated benthic foraminifera from surface samples collected in the San Pedro and Santa Catalina Basins reveals a predictable relationship between the proportions of morphogroups with decreasing bottom water oxygen levels and with the TOC content of the surficial sediment. Living (Rose Bengal stained) foraminiferal faunas from dysaerobic environments display low diversity and high dominance, suggesting stressed conditions. There is an inverse relationship between oxygen and the relative abundance of deep infaunal morphogroups. Samples collected from shallow stations above the oxygen minimum zone are comprised of epifaunal and shallow infaunal morphotypes. At intermediate depths (~500 m), there is a peak in the abundance of suspension-feeding and "climbing" forms (watchglass-shaped trochamminids attached to Rhabdammina). Specimens from intermediate stations display the largest overall size. Deeper in the San Pedro Basin the living fauna is dominated by a small, flattened, tapered, species that is interpreted as having a deep infaunal microhabitat. In the dysaerobic environments off California the greatest degree of faunal change occurs when bottom water dissolved oxygen values drop from 0.5 ml/l to 0.2 ml/l. The effect of TOC content on the benthic fauna is demonstrated at two stations from the same depth in the San Pedro Basin. The station with the higher TOC content (4.2% vs. 2.9%) contains greater proportions of the small, deep infaunal morphotype. These faunal changes may be attributed to differences in the depth of the oxygenated zone within the sediment surface layer. Agglutinated faunas from areas that experience seasonal anoxia are comprised of a large proportion of opportunistic forms such as Reophax and Psammosphaera. These are the same taxa that colonised abiotic sediment trays in a recolonisation experiment in the Panama Basin. This study further demonstrates that agglutinated foraminiferal morphotypes respond in a similar manner to calcareous benthic foraminifera in dysaerobic environments

Intact Bilateral Resting-State Networks in the Absence of the Corpus Callosum

Temporal correlations between different brain regions in the resting-state BOLD signal are thought to reflect intrinsic functional brain connectivity (Biswal et al., 1995; Greicius et al., 2003; Fox et al., 2007). The functional networks identified are typically bilaterally distributed across the cerebral hemispheres, show similarity to known white matter connections (Greicius et al., 2009), and are seen even in anesthetized monkeys (Vincent et al., 2007). Yet it remains unclear how they arise. Here we tested two distinct possibilities: (1) functional networks arise largely from structural connectivity constraints, and generally require direct interactions between functionally coupled regions mediated by white-matter tracts; and (2) functional networks emerge flexibly with the development of normal cognition and behavior and can be realized in multiple structural architectures. We conducted resting-state fMRI in eight adult humans with complete agenesis of the corpus callosum (AgCC) and normal intelligence, and compared their data to those from eight healthy matched controls. We performed three main analyses: anatomical region-of-interest-based correlations to test homotopic functional connectivity, independent component analysis (ICA) to reveal functional networks with a data-driven approach, and ICA-based interhemispheric correlation analysis. Both groups showed equivalently strong homotopic BOLD correlation. Surprisingly, almost all of the group-level independent components identified in controls were observed in AgCC and were predominantly bilaterally symmetric. The results argue that a normal complement of resting-state networks and intact functional coupling between the hemispheres can emerge in the absence of the corpus callosum, favoring the second over the first possibility listed above

Regional requirements for Dishevelled signaling during Xenopus gastrulation: separable effects on blastopore closure, mesendoderm internalization and archenteron formation

During amphibian gastrulation, the embryo is transformed by the combined actions of several different tissues. Paradoxically, many of these morphogenetic processes can occur autonomously in tissue explants, yet the tissues in intact embryos must interact and be coordinated with one another in order to accomplish the major goals of gastrulation: closure of the blastopore to bring the endoderm and mesoderm fully inside the ectoderm, and generation of the archenteron. Here, we present high-resolution 3D digital datasets of frog gastrulae, and morphometrics that allow simultaneous assessment of the progress of convergent extension, blastopore closure and archenteron formation in a single embryo. To examine how the diverse morphogenetic engines work together to accomplish gastrulation, we combined these tools with time-lapse analysis of gastrulation, and examined both wild-type embryos and embryos in which gastrulation was disrupted by the manipulation of Dishevelled (Xdsh) signaling. Remarkably, although inhibition of Xdsh signaling disrupted both convergent extension and blastopore closure, mesendoderm internalization proceeded very effectively in these embryos. In addition, much of archenteron elongation was found to be independent of Xdsh signaling, especially during the second half of gastrulation. Finally, even in normal embryos, we found a surprising degree of dissociability between the various morphogenetic processes that occur during gastrulation. Together, these data highlight the central role of PCP signaling in governing distinct events of Xenopus gastrulation, and suggest that the loose relationship between morphogenetic processes may have facilitated the evolution of the wide variety of gastrulation mechanisms seen in different amphibian species

Plectoeratidus subarcticus, n.gen., n.sp., a new agglutinated foraminifer from the Upper Cretaceous of the western Barents Sea

We describe the new foraminiferal genus and species Plectoeratidus subarcticus n.gen. n.sp., from the Upper Cretaceous deep-water deposits of the Kviting and Kveite formations in the western Barents Sea. The genus is characterised by its planispiral-biserial-uniserial chamber arrangement, terminal aperture and lateral compression. The biometric analysis of test ontogenesis has been applied to document its dimorphism attributed to megalospheric and microspheric generations. This taxon may represent an evolutionary transition between the Cretaceous genera Spiroplectammina or Bolivinopsis and the Cenozoic genus Eratidus. The taxon appears to be endemic to the flysch-type agglutinated foraminiferal assemblages in the Norwegian Sea area

Meat, wool and milk utilization together with comparison of F1 coming from Finnsheep rams with F1 coming from other prolific breeds

The effect of crossing of Polish Corriedale (C) sheep with East Friesian, Finnsheep (F), and Polish Heath sheep was analyzed. The utility of F1 crossbreds coming from C and prolific breed rams (given above) was confirmed. The F, crosses of C ewes and F rams were characterized by the best reproductive performance, high level of milk production and the small decrease of wool quality and quantity produced by the ewes, and good carcase quality of ram lambs

Brain Differences in the Prefrontal Cortex, Amygdala, and Hippocampus in Youth with Congenital Adrenal Hyperplasia

Context: Classical Congenital Adrenal Hyperplasia (CAH) due to 21-hydroxylase deficiency results in hormone imbalances present both prenatally and postnatally that may impact the developing brain. Objective: To characterize gray matter morphology in the prefrontal cortex and subregion volumes of the amygdala and hippocampus in youth with CAH, compared to controls. Design: A cross-sectional study of 27 CAH youth (16 female; 12.6 ± 3.4 year) and 35 typically developing, healthy controls (20 female; 13.0 ± 2.8 year) with 3-T magnetic resonance imaging scans. Brain volumes of interest included bilateral prefrontal cortex, and nine amygdala and six hippocampal subregions. Between-subject effects of group (CAH vs control) and sex, and their interaction (group-by-sex) on brain volumes were studied, while controlling for intracranial volume (ICV) and group differences in body mass index and bone age. Results: CAH youth had smaller ICV and increased cerebrospinal fluid volume compared to controls. In fully-adjusted models, CAH youth had smaller bilateral, superior and caudal middle frontal volumes, and smaller left lateral orbito-frontal volumes compared to controls. Medial temporal lobe analyses revealed the left hippocampus was smaller in fully-adjusted models. CAH youth also had significantly smaller lateral nucleus of the amygdala and hippocampal subiculum and CA1 subregions. Conclusions: This study replicates previous findings of smaller medial temporal lobe volumes in CAH patients, and suggests that lateral nucleus of the amygdala, as well as subiculum and subfield CA1 of the hippocampus are particularly affected within the medial temporal lobes in CAH youth

Plasma progesterone concentration during oestrus cycle and pregnancy in Finnsheep and other prolific sheep breeds

The aim of the study was to evaluate the relationship between the litter size and plasma progesterone (PP) concentration during the breeding season and pregnancy in some prolific sheep breeds. Investigations were performed during two consecutive reproductive cycles. PP concentrations were determined in Finnsheep (n = 16), Polish Heath (n = 13), Friesian (n = 10), Zelazna (n = 12) and Karakul (n = 14). Prolificacy was 3.14, 2.0, 2.0, 1.8 and 1.12, resp. Highly significant differences in PP levels during pregnancy were found between the prolific breeds and breed with the lowest litter size. There was also a significant effect of pregnancy stage on the PP concentration. Breed differences in PP levels during the oestrus cycle were the most pronounced on the 10th day of the cycle. However, due to a considerable variation within breeds they were statistically insignificant

The Claustrum and Insula in Microcebus murinus: A High Resolution Diffusion Imaging Study

The claustrum and the insula are closely juxtaposed in the brain of the prosimian primate, the gray mouse lemur (Microcebus murinus). Whether the claustrum has closer affinities with the cortex or the striatum has been debated for many decades. Our observation of histological sections from primate brains and genomic data in the mouse suggest former. Given this, the present study compares the connections of the two structures in Microcebus using high angular resolution diffusion imaging (HARDI, with 72 directions), with a very small voxel size (90 micra), and probabilistic fiber tractography. High angular and spatial resolution diffusion imaging is non-destructive, requires no surgical interventions, and the connection of each and every voxel can be mapped, whereas in conventional tract tracer studies only a few specific injection sites can be assayed. Our data indicate that despite the high genetic and spatial affinities between the two structures, their connectivity patterns are very different. The claustrum connects with many cortical areas and the olfactory bulb; its strongest probabilistic connections are with the entorhinal cortex, suggesting that the claustrum may have a role in spatial memory and navigation. By contrast, the insula connects with many subcortical areas, including the brainstem and thalamic structures involved in taste and visceral feelings. Overall, the connections of the Microcebus claustrum and insula are similar to those of the rodents, cat, macaque, and human, validating our results. The insula in the Microcebus connects with the dorsolateral frontal cortex in contrast to the mouse insula, which has stronger connections with the ventromedial frontal lobe, yet this is consistent with the dorsolateral expansion of the frontal cortex in primates. In addition to revealing the connectivity patterns of the Microcebus brain, our study demonstrates that HARDI, at high resolutions, can be a valuable tool for mapping fiber pathways for multiple sites in fixed brains in rare and difficult-to-obtain species

A high-resolution probabilistic in vivo atlas of human subcortical brain nuclei

Recent advances in magnetic resonance imaging methods, including data acquisition, pre-processing and analysis, have benefited research on the contributions of subcortical brain nuclei to human cognition and behavior. At the same time, these developments have led to an increasing need for a high-resolution probabilistic in vivo anatomical atlas of subcortical nuclei. In order to address this need, we constructed high spatial resolution, three-dimensional templates, using high-accuracy diffeomorphic registration of T_1- and T_2- weighted structural images from 168 typical adults between 22 and 35 years old. In these templates, many tissue boundaries are clearly visible, which would otherwise be impossible to delineate in data from individual studies. The resulting delineations of subcortical nuclei complement current histology-based atlases. We further created a companion library of software tools for atlas development, to offer an open and evolving resource for the creation of a crowd-sourced in vivoprobabilistic anatomical atlas of the human brain

The autism brain imaging data exchange: towards a large-scale evaluation of the intrinsic brain architecture in autism

Autism spectrum disorders (ASDs) represent a formidable challenge for psychiatry and neuroscience because of their high prevalence, lifelong nature, complexity and substantial heterogeneity. Facing these obstacles requires large-scale multidisciplinary efforts. Although the field of genetics has pioneered data sharing for these reasons, neuroimaging had not kept pace. In response, we introduce the Autism Brain Imaging Data Exchange (ABIDE)—a grassroots consortium aggregating and openly sharing 1112 existing resting-state functional magnetic resonance imaging (R-fMRI) data sets with corresponding structural MRI and phenotypic information from 539 individuals with ASDs and 573 age-matched typical controls (TCs; 7–64 years) (http://fcon_1000.projects.nitrc.org/indi/abide/). Here, we present this resource and demonstrate its suitability for advancing knowledge of ASD neurobiology based on analyses of 360 male subjects with ASDs and 403 male age-matched TCs. We focused on whole-brain intrinsic functional connectivity and also survey a range of voxel-wise measures of intrinsic functional brain architecture. Whole-brain analyses reconciled seemingly disparate themes of both hypo- and hyperconnectivity in the ASD literature; both were detected, although hypoconnectivity dominated, particularly for corticocortical and interhemispheric functional connectivity. Exploratory analyses using an array of regional metrics of intrinsic brain function converged on common loci of dysfunction in ASDs (mid- and posterior insula and posterior cingulate cortex), and highlighted less commonly explored regions such as the thalamus. The survey of the ABIDE R-fMRI data sets provides unprecedented demonstrations of both replication and novel discovery. By pooling multiple international data sets, ABIDE is expected to accelerate the pace of discovery setting the stage for the next generation of ASD studies