Additional file 1 of Vesicular HMGB1 release from neurons stressed with spreading depolarization enables confined inflammatory signaling to astrocytes

Additional file 1: Figure S1. Gating strategy applied to flow cytometry. Forward and side scatter gating is used in flow cytometry analysis to identify the single beads based on the relative size and complexity (clumping) of the beads while removing debris and other events that are not of interest. The mean fluorescence intensity was used as a quantitative measure