21 research outputs found
OC-0482: Intraoperative electron boost compensates adverse prognostic factors for pelvic recurrences in rectal cancer
Lack of intimal hyperplasia response in an experimental model of non-endothelial vascular wall damage
The endothelial and media1 layers are
generally presumed to play an important role in the
appearance and development of intimal hyperplasia.
We have carried out a short-, media- and long-term
study of the morphological changes taking place in the
comrnon iliac artery of rats after surgical removal of the
adventitial layer. Our aim has been to assess the likely
role played by this layer in the development of intimal
hyperplasia.
Our results show recurrent periods of cellular
desquamation and almost complete absence of
hyperplastic response during the first two months. After
three months three is a slow process of
endothelialization which is completed by the 6th month
and persists one year after adventitial resection.
Thus, adventitial resection seems to cause instability
at the subendothelial bed level, not allowing the junction
and embedding of endothelial cells nor the development
of intimal hyperplasia.
This lack of hyperplasia might also result from the
fact that the endothelial desquamation process does not
involve cellular rupture, which would prevent
mitogenic-factor release.
After morphological repair of the endothelium, a
slow morphofunctional recovery of the artery takes
plac
Seeding of expanded polytetrafluoroethylene (ePTFE) vascular grafts. A morphological study of porcine endothelial and fibroblast cells
The need to improve clinical results with
small and medium calibre grafts has led to extensive
research on cell seeding of prosthetic materials.
Numerous problems remain regarding identification,
seeding, adhesion and survival of the cells attached.
We have studied the behaviour of seedings of
endothelial and fibroblast cells on ePTFE grafts.
Scanning electron microscopy allows us to observe
the morphological characteristics and their interaction
with the biopolymers. It has been possible to
differentiate both cellular types by their characteristics
and interactions with the ePTFE. At the same time, from
this ~ i vni t ro~st udy it can be concluded that the time
needed to obtain a stable and confluent monolayer on
ePTFE pretreated with fibronectin is between 18 hours
to 4 days for endothelial cells, and 24 hours for
fibroblasts. These would be the optimal time periods for
ain vivo. grafting of the seeded prostheses