Breast tumor kinase (Brk/PTK6) plays a role in the differentiation of primary keratinocytes

This work was supported by a project grant from the Biotechnology and Biological Sciences Research Council, UKBreast Tumor Kinase (Brk/PTK6) has a relatively limited expression profile in normal tissue. Its expression is restricted to epithelial cells that are differentiating such as those in the epidermis, and Brk expression appears to be absent from proliferating cells in normal tissue. Also, there is now some evidence to suggest that Brk plays a functional role in the differentiation of the keratinocytes in the epidermis. We have, therefore, investigated the role that Brk/PTK6 plays in normal human primary keratinocytes by suppressing protein levels using RNA interference. We show that as primary human keratinocytes are induced to differentiate in vitro, Brk levels decrease. Decreasing Brk protein levels lead to an increase in the number of cells with a permeable plasma membrane, a decrease in epidermal growth factor receptor (EGFR) and a parallel increase in keratin 10 levels, but classical markers of apoptosis or terminal differentiation are not affected. We propose Brk, Keratin 10 and EGFR are co-regulated during differentiation and that manipulating Brk expression can influence the differentiation of normal primary human keratinocytes.This article is available through the Brunel University Open Access Publishing Fund

The Relation between Approximation in Distribution and Shadowing in Molecular Dynamics

Molecular dynamics refers to the computer simulation of a material at the atomic level. An open problem in numerical analysis is to explain the apparent reliability of molecular dynamics simulations. The difficulty is that individual trajectories computed in molecular dynamics are accurate for only short time intervals, whereas apparently reliable information can be extracted from very long-time simulations. It has been conjectured that long molecular dynamics trajectories have low-dimensional statistical features that accurately approximate those of the original system. Another conjecture is that numerical trajectories satisfy the shadowing property: that they are close over long time intervals to exact trajectories but with different initial conditions. We prove that these two views are actually equivalent to each other, after we suitably modify the concept of shadowing. A key ingredient of our result is a general theorem that allows us to take random elements of a metric space that are close in distribution and embed them in the same probability space so that they are close in a strong sense. This result is similar to the Strassen-Dudley Theorem except that a mapping is provided between the two random elements. Our results on shadowing are motivated by molecular dynamics but apply to the approximation of any dynamical system when initial conditions are selected according to a probability measure.Comment: 21 pages, final version accepted in SIAM Dyn Sy

A classification of spherically symmetric spacetimes

A complete classification of locally spherically symmetric four-dimensional Lorentzian spacetimes is given in terms of their local conformal symmetries. The general solution is given in terms of canonical metric types and the associated conformal Lie algebras. The analysis is based upon the local conformal decomposition into 2+2 reducible spacetimes and the Petrov type. A variety of physically meaningful example spacetimes are discussed

Chaplygin Gas Cosmology - Unification of Dark Matter and Dark Energy

The models that unify dark matter and dark energy based upon the Chaplygin gas fail owing to the suppression of structure formation by the adiabatic speed of sound. Including string theory effects, in particular the Kalb-Ramond field, we show how nonadiabatic perturbations allow a successful structure formation.Comment: 7 pages, presented by N. B. at IRGAC 2006, Barcelona, 11-15 July 2006, typos corrected, concluding paragraph slightly expanded, final version, accepted in J. Phys. A, special issu

Gauging the Shadow Sector with SO(3)

We examine the phenomenology of a low-energy extension of the Standard Model, based on the gauge group SU(3) x SU(2) x U(1) x SO(3), with SO(3) operating in the shadow sector. This model offers $\nu_{e} \to \nu_{s}$ and $\nu_{\mu} \to \nu_{\tau}$ oscillations as the solution of the solar and atmospheric neutrino problems. Moreover, it provides a neutral heavy shadow lepton X that could play the role of a cold dark matter particle.Comment: 8 page

On the dual interpretation of zero-curvature Friedmann-Robertson-Walker models

Two possible interpretations of FRW cosmologies (perfect fluid or dissipative fluid)are considered as consecutive phases of the system. Necessary conditions are found, for the transition from perfect fluid to dissipative regime to occur, bringing out the conspicuous role played by a particular state of the system (the ''critical point '').Comment: 13 pages Latex, to appear in Class.Quantum Gra