Additional file 1: of Effect of Enhanced Thermal Stability of Alumina Support Layer on Growth of Vertically Aligned Single-Walled Carbon Nanotubes and Their Application in Nanofiltration Membranes

Figure S1. AFM on an unstable alumina film annealed at 750 °C. Figure S2. Al 2p XPS spectra of the unstable and the stable alumina films. Figure S3. Diameter distribution of the catalyst particles after annealing extracted from AFM image analysis (bars). Top: unstable alumina films; Bottom: stable alumina films. Overlapping dashed curves represent the corresponding SWCNT diameter distribution measured by TEM. Table S1. Typical methods used in the literature to verify that transport occurs through CNTs rather than through defects in the matrix. NP = nanoparticle; P gas  = gas permeance. (DOCX 459 kb

Genetic map of pMET1 and comparison to plasmid pCRY and chromosomal elements.

<p>A. The genetic maps of pMET1 and pCRY are compared showing the homologous regions. The arrows indicate genes locations and orientation. Genes with different functions are shown with different colors and if the genes in the different structures shown are homologus they are represented with the same colors. Yellow: mobilization; green: replication and partition; red: antibiotic resistance; purple: virB/pilX-like; blue: transposition; grey: unknown. Since pCRY is smaller than pMET1, to represent it in circular form a dotted line was added to fill the gap. Solid line represents non-homologous DNA. B. Comparison of a pMET1 region with chromosomal HPIs or ICEs is shown using a linearized version of the plasmid. The HPIs shown are those from <i>E. coli</i> ECOR31 (HPI_ECOR31) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001800#pone.0001800-Schubert1" target="_blank">[43]</a>, <i>K. pneumoniae</i> NTUH-K2044 (ICE_Kp1) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001800#pone.0001800-Lin1" target="_blank">[44]</a>, and <i>Y. pestis</i> KIM (HPI_Yp)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001800#pone.0001800-Schubert1" target="_blank">[43]</a>. The diagram shows the HP core regions, which are not at scale and are represented as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001800#pone.0001800-Schubert1" target="_blank">[43]</a>, and the RB-HPIs. The sequence described in this manuscript has been deposited in GenBank, accession number is EU383016.</p

Replication region of pMET1.

<p>A. The bar shows a genetic map of the pMET1 replication region and the GC content plot generated using a window size of 100 bp on top. Recombinant clones were obtained by inserting the indicated fragments into pCR2.1 or ligated to the pUC4K <i>aph</i> cassette. The ability to be maintained in <i>E. coli</i> C2110 (a <i>polA</i> mutant) of the recombinant plasmids made using pCR2.1 as vector is indicated to the right by a + or − sign. The ability to generate kanamycin resistant colonies in <i>E. coli</i> TOP10 of the indicated fragments when ligated to the <i>aph</i> cassette from pUC4K is also represented by a + or − sign. B. BLASTP comparison of the amino acid sequences of the putative RepA proteins from pMET1 and pCRY.</p